LOCUS       sequence-197173-        9048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sox4 coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, 
            Iacobuzio-Donahue CA, Massague J
  TITLE     TGF-beta Tumor Suppression through a Lethal EMT.
  JOURNAL   Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. 
            Epub 2016 Feb 18.
  PUBMED    26898331
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.009"; journalName: "Cell"; date: 
            "2016-02-25- 25"; volume: "164"; issue: "5"; pages: "1015-30"
COMMENT     Sequence Label: pLVXT Sox4
FEATURES             Location/Qualifiers
     source          1..9048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..326
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    56..74
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    198..216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     299..323
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1687..2186
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1722..1745)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2119..2138
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2207..2806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2207..2226)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2703..2723
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2823..3411
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2876..2896)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3294..3305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3618..4251
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4379..4395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4379..4395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4392..4414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4403..4419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4427..4433,4434..4451,4452..4457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4472..4493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4610..4627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4781..5369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4861..4880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5540..6331,6332..6400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6163..6182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6401..6505)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6553..6687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6590..6609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6644..6663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6855..7488
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7535..7660
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8157..8390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8575..8619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8882..8997
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgtcttcact cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
       61 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      121 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      181 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
      241 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
      301 ctcgtttagt gaaccgtcag atcgcctgga gaaggatcga tccgccacca tggtacaaca
      361 gaccaacaac gcggagaaca ctgaggctct gctggccggg gagagctcgg actcgggcgc
      421 cggcctggag ctgggcatcg cgtcctcccc gacgcctggc tccaccgcgt cgacgggcgg
      481 caaggcggac gaccccagct ggtgcaagac gcccagtggc cacatcaagc ggcccatgaa
      541 cgcctttatg gtgtggtcgc agatcgagcg gcgcaagatc atggagcagt cgcccgacat
      601 gcacaacgcc gagatctcca agcggctagg caaacgctgg aagctgctca aggacagcga
      661 caagattccg ttcatccagg aggcggagcg gctgcgcctc aagcacatgg ctgactaccc
      721 tgactacaag taccggccgc gaaagaaggt gaagtcgggc aacgcgggcg cgggatcggc
      781 ggccacagcc aagccagggg agaagggcga caaggtcgcg ggcagcagcg gccacgcggg
      841 aagcagccac gcggggggtg gcgcgggcgg cagctccaag cccgcgccca agaagagctg
      901 tggccccaag gtggcgggca gctcggtcgg caagccccac gctaagctgg tcccggcggg
      961 cggcagcaag gcggctgcat cgttctctcc agagcaagct gccctgctgc ccctggggga
     1021 gcccacggcc gtctacaagg tgcggactcc cagtgcggcc actccggccg cctcctcctc
     1081 gccgtccagt gcgctggcca ccccagccaa acaccctgcc gacaagaaag tgaagcgcgt
     1141 ctacctgttt ggaagcctgg gcgcttcggc gtctcccgtc gggggcctgg gagcgagcgc
     1201 cgaccccagt gatccactgg ggttgtacga agatggaggc ccgggatgct cgcccgatgg
     1261 ccggagtctg agcggccgca gcagcgcagc atcatcgcca gccgccagcc gatcgcccgc
     1321 tgaccaccgc ggctacgcca gcctacgcgc agcctcgccc gccccgtcca gcgcgccctc
     1381 gcacgcgtcc tcctcgctct cctcgtcctc ttcctcctcc tcgggctctt cgtcgtccga
     1441 cgacgagttc gaagacgacc tgctcgacct gaaccccagc tcaaactttg agagcatgtc
     1501 cctgggcagt ttcagctcct catcggcgct cgatcgggac ctggatttta acttcgaacc
     1561 cggctcaggc tcccacttcg aattcccgga ctattgcacg cccgaggtga gcgagatgat
     1621 ctcgggagat tggctggagt ccagcatctc taacctggtc ttcacctact gaggatcaat
     1681 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     1741 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     1801 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     1861 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     1921 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     1981 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     2041 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     2101 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     2161 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     2221 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     2281 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     2341 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     2401 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     2461 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     2521 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     2581 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     2641 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     2701 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     2761 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacggg cgcgtctgga
     2821 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2881 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2941 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3001 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3061 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3121 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3181 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3241 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3301 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3361 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     3421 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     3481 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     3541 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     3601 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     3661 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     3721 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     3781 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     3841 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     3901 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     3961 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     4021 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     4081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     4261 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4321 ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt
     4381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     6541 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6601 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6661 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     6721 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     6781 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     6841 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     6901 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     6961 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     7021 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     7081 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     7141 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     7201 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     7261 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     7321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7381 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     7501 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     7561 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     7621 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7681 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     7741 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7801 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7861 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7921 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7981 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     8041 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8101 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8161 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8221 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8281 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8341 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8401 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8461 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8521 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8581 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8641 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8701 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8761 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     8821 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     8881 ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     8941 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9001 ggtttattac agggacagca gagatccagt ttatcgatga ggcccttt
//
