LOCUS       sequence-197198-        8332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to transmembrane 
            tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65...
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          5..232
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     sig_peptide     254..313
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             356..385
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             389..535
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             617..637
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             638..658
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             677..703
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(731..1711,1712..1714,1715..3229)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             3233..3253
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3272..3421
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3449..3502
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3518..3904
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             3959..3979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3995..4537
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4562..4933
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(4961..4978)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4967..5174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5183..5323
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5183..5312
                     /label=AAV2 ITR
     rep_origin      5398..5853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5485..5504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5695..5716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5870..5889)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5989..6011
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6049..6067)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6135..6239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6240..6308,6309..7100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6458..6477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7271..7859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7760..7779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7931..8216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
       61 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      121 tcctccgggc tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg
      181 ggtattaatg tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc
      241 ggtgatatcc accatggaga cagacacact cctgctatgg gtactgctgc tctgggttcc
      301 aggttccact ggtgaccatt ctggtggcgg aagtggggga ggcagcggtc gacaagaaca
      361 aaaactcatc tcagaagagg atctgaatgc tgtgggccag gacacgcagg aggtcatcgt
      421 ggtgccacac tccttgccct ttaaggtggt ggtgatctca gccatcctgg ccctggtggt
      481 gctcaccatc atctccctta tcatcctcat catgctttgg cagaagaagc cacgtctgca
      541 gagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtg ctagccatgt
      601 cgaccatgcg gccgctgaaa acctgtattt tcaggggccc aagaagaaga ggaaggtggg
      661 gggaggcagc actagttacc catacgacgt accagattac gctggaggtt ctggatccgg
      721 cggtggctcg aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
      781 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
      841 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
      901 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
      961 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     1021 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     1081 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     1141 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     1201 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     1261 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     1321 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     1381 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     1441 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     1501 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     1561 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     1621 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     1681 cgacaacaag gtgctgacca gaagcgacaa ggcccggggc aagagcgaca acgtgccctc
     1741 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     1801 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     1861 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     1921 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     1981 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     2041 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     2101 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     2161 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     2221 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     2281 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     2341 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     2401 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     2461 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     2521 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     2581 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     2641 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     2701 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     2761 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     2821 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     2881 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     2941 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     3001 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     3061 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     3121 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     3181 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca gccccaagaa
     3241 gaagagaaag gtggaggcca gcggacgggc tgacgcattg gacgattttg atctggatat
     3301 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga
     3361 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct
     3421 gattaacggt accgctagcg gcagtggaga gggcagagga agtctgctaa catgcggtga
     3481 cgtcgaggag aatcctggcc cagtgagcaa gcttatggct tcaaacttta ctcagttcgt
     3541 gctcgtggac aatggtggga caggggatgt gacagtggct ccttctaatt tcgctaatgg
     3601 ggtggcagag tggatcagct ccaactcacg gagccaggcc tacaaggtga catgcagcgt
     3661 caggcagtct agtgcccaga agagaaagta taccatcaag gtggaggtcc ccaaagtggc
     3721 tacccagaca gtgggcggag tcgaactgcc tgtcgccgct tggaggtcct acctgaacat
     3781 ggagctcact atcccaattt tcgctaccaa ttctgactgt gaactcatcg tgaaggcaat
     3841 gcaggggctc ctcaaagacg gtaatcctat cccttccgcc atcgccgcta actcaggtat
     3901 ctacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggacc
     3961 taagaaaaag aggaaggtgg cggccgctgg atccccttca gggcagatca gcaaccaggc
     4021 cctggctctg gcccctagct ccgctccagt gctggcccag actatggtgc cctctagtgc
     4081 tatggtgcct ctggcccagc cacctgctcc agcccctgtg ctgaccccag gaccacccca
     4141 gtcactgagc gctccagtgc ccaagtctac acaggccggc gaggggactc tgagtgaagc
     4201 tctgctgcac ctgcagttcg acgctgatga ggacctggga gctctgctgg ggaacagcac
     4261 cgatcccgga gtgttcacag atctggcctc cgtggacaac tctgagtttc agcagctgct
     4321 gaatcagggc gtgtccatgt ctcatagtac agccgaacca atgctgatgg agtaccccga
     4381 agccattacc cggctggtga ccggcagcca gcggcccccc gaccccgctc caactcccct
     4441 gggaaccagc ggcctgccta atgggctgtc cggagatgaa gacttctcaa gcatcgctga
     4501 tatggacttt agtgccctgc tgtcacagat ttcctctagt gggcagggag gaggtggaag
     4561 cggcttcagc gtggacacca gtgccctgct ggacctgttc agcccctcgg tgaccgtgcc
     4621 cgacatgagc ctgcctgacc ttgacagcag cctggccagt atccaagagc tcctgtctcc
     4681 ccaggagccc cccaggcctc ccgaggcaga gaacagcagc ccggattcag ggaagcagct
     4741 ggtgcactac acagcgcagc cgctgttcct gctggacccc ggctccgtgg acaccgggag
     4801 caacgacctg ccggtgctgt ttgagctggg agagggctcc tacttctccg aaggggacgg
     4861 cttcgccgag gaccccacca tctccctgct gacaggctcg gagcctccca aagccaagga
     4921 ccccactgtc tcctaagaat tcctagagct cgctgatcag cctcgactgt gccttctagt
     4981 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5041 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5101 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc
     5161 aggcatgctg gggagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     5221 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     5281 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     5341 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     5401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5461 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5581 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     5641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5701 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     5761 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5821 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     5881 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5941 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6001 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6061 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6241 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6361 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6421 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6481 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6541 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6601 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6781 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6841 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6901 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc
     6961 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7081 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7141 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7381 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7861 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgc
     7921 tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7981 cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     8041 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     8101 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct
     8161 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     8221 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     8281 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gg
//
