LOCUS       sequence-197199-        8491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to transmembrane 
            tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65...
FEATURES             Location/Qualifiers
     source          1..8491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..804
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     sig_peptide     826..885
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             928..957
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             961..1107
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             1189..1209
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1210..1230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1249..1275
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1303..2283,2284..2286,2287..3801)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             3805..3825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3844..3993
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4021..4074
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4090..4476
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4531..4551
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4567..5109
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5134..5505
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(5533..5550)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5539..5746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5755..5895
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5755..5884
                     /label=AAV2 ITR
     rep_origin      5970..6425
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6057..6076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6267..6288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6442..6461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6561..6583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6621..6639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6707..6811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6812..6880,6881..7672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7030..7049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7843..8431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8332..8351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
      721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
      781 ctgcctgaaa tcactttttt tcaggttgga ccggtgatat ccaccatgga gacagacaca
      841 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacca ttctggtggc
      901 ggaagtgggg gaggcagcgg tcgacaagaa caaaaactca tctcagaaga ggatctgaat
      961 gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc ctttaaggtg
     1021 gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct tatcatcctc
     1081 atcatgcttt ggcagaagaa gccacgtctg cagagcggca gcgagactcc cgggacctca
     1141 gagtccgcca cacccgaaag tgctagccat gtcgaccatg cggccgctga aaacctgtat
     1201 tttcaggggc ccaagaagaa gaggaaggtg gggggaggca gcactagtta cccatacgac
     1261 gtaccagatt acgctggagg ttctggatcc ggcggtggct cgaagcccgc cttcctgagc
     1321 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     1381 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     1441 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     1501 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     1561 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     1621 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     1681 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     1741 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     1801 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     1861 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     1921 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     1981 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     2041 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     2101 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     2161 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     2221 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     2281 aaggcccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     2341 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     2401 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     2461 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     2521 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     2581 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     2641 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     2701 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     2761 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     2821 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     2881 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     2941 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     3001 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     3061 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     3121 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     3181 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     3241 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     3301 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     3361 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     3421 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     3481 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     3541 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     3601 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     3661 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     3721 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     3781 ctgtctcagc tgggaggcga cagccccaag aagaagagaa aggtggaggc cagcggacgg
     3841 gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt
     3901 gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt
     3961 gacgcccttg atgatttcga cctggacatg ctgattaacg gtaccgctag cggcagtgga
     4021 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccagtgagc
     4081 aagcttatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
     4141 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
     4201 cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgccca gaagagaaag
     4261 tataccatca aggtggaggt ccccaaagtg gctacccaga cagtgggcgg agtcgaactg
     4321 cctgtcgccg cttggaggtc ctacctgaac atggagctca ctatcccaat tttcgctacc
     4381 aattctgact gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga cggtaatcct
     4441 atcccttccg ccatcgccgc taactcaggt atctacagcg ctggaggagg tggaagcgga
     4501 ggaggaggaa gcggaggagg aggtagcgga cctaagaaaa agaggaaggt ggcggccgct
     4561 ggatcccctt cagggcagat cagcaaccag gccctggctc tggcccctag ctccgctcca
     4621 gtgctggccc agactatggt gccctctagt gctatggtgc ctctggccca gccacctgct
     4681 ccagcccctg tgctgacccc aggaccaccc cagtcactga gcgctccagt gcccaagtct
     4741 acacaggccg gcgaggggac tctgagtgaa gctctgctgc acctgcagtt cgacgctgat
     4801 gaggacctgg gagctctgct ggggaacagc accgatcccg gagtgttcac agatctggcc
     4861 tccgtggaca actctgagtt tcagcagctg ctgaatcagg gcgtgtccat gtctcatagt
     4921 acagccgaac caatgctgat ggagtacccc gaagccatta cccggctggt gaccggcagc
     4981 cagcggcccc ccgaccccgc tccaactccc ctgggaacca gcggcctgcc taatgggctg
     5041 tccggagatg aagacttctc aagcatcgct gatatggact ttagtgccct gctgtcacag
     5101 atttcctcta gtgggcaggg aggaggtgga agcggcttca gcgtggacac cagtgccctg
     5161 ctggacctgt tcagcccctc ggtgaccgtg cccgacatga gcctgcctga ccttgacagc
     5221 agcctggcca gtatccaaga gctcctgtct ccccaggagc cccccaggcc tcccgaggca
     5281 gagaacagca gcccggattc agggaagcag ctggtgcact acacagcgca gccgctgttc
     5341 ctgctggacc ccggctccgt ggacaccggg agcaacgacc tgccggtgct gtttgagctg
     5401 ggagagggct cctacttctc cgaaggggac ggcttcgccg aggaccccac catctccctg
     5461 ctgacaggct cggagcctcc caaagccaag gaccccactg tctcctaaga attcctagag
     5521 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5581 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5641 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5701 acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggagcgg ccgcaggaac
     5761 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     5821 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     5881 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5941 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     6001 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     6061 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6121 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6181 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6241 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6301 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     6361 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     6421 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     6481 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     6541 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     6601 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6661 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6781 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6841 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6901 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6961 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7021 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7081 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7141 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7201 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7261 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7321 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7381 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7441 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7501 tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga
     7561 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7621 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7681 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7741 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7981 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8461 cctggccttt tgctggcctt ttgctcacat g
//
