LOCUS       sequence-197203-        2758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conductive Curli Protein csgAW.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Kalyoncu E, Ahan RE, Olmez TT, Seker UOS
  TITLE     Genetically encoded conductive protein nanofibers secreted by 
            engineered cells
  JOURNAL   RSC Adv. 2017, Issue 52. doi: 10.1039/C7RA06289C
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RSC Adv. 
            2017, Issue 52. doi: 10.1039/C7RA06289C"
COMMENT     Sequence Label: pZa-tetO-csgAW-CmR
FEATURES             Location/Qualifiers
     source          1..2758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             176..187
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(222..247,248..253,254..270,271..276,
                     277..295))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    252..270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    277..295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    548..566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    573..591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        630..732
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             733..1392
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(799..818)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1413..1507
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1621..2166
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2067..2086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2283..2300
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2335..2421)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcagagtt accgccaccg tactggtaaa tgttcagctc agaatttggg ccgctattat
       61 taccgccacc accgtggtta ccgccgccgc cgtactgagg aacaacacct gccagagcgc
      121 taccggagaa tacgattgct gcaattgctg ctacttttaa aagtttcatg gtacctttct
      181 cctctttaat acccaaatcc aagaggtgaa tggtagaatt cggtcagtgc gtcctgctga
      241 tgtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat agggactcga
      301 gaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc
      361 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca
      421 tggggtcagg tgggaccacc gcgctactgc cgccaggcat ctagagatat atggtagtag
      481 taagttaatt ttcattaacc accactacca atcacctcct ggatttgggt gtcgactgtg
      541 ctcagtatct ctatcactga tagggatgtc aatctctatc actgataggg agggcccttt
      601 gacgtcgata tctggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      661 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      721 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      781 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      841 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      901 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      961 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1021 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1081 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1141 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1201 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1261 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1321 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1381 ggcggggcgt aatttgatat cgagctcgct tggactcctg ttgatagatc cagtaatgac
     1441 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     1501 tgagaatcca agcactagta acaacttata tcgtatgggg ctgacttcag gtgctacatt
     1561 tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc
     1621 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
     1681 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
     1741 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
     1801 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
     1861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
     1921 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     1981 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     2041 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
     2101 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
     2161 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
     2221 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
     2281 gtagcgagtc agtgagcgag gaagcggaat atatccctag gtctagggcg gcggatttgt
     2341 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     2401 ctgagccttt cgttttattt gatgcctcta gcacgcgttt accagtcatc ccattctttc
     2461 catttacgcc accagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg
     2521 ttgacggagg agttagatgc agtctggtca actgctgcac cgttgccacc accgaactgt
     2581 ttaaccgtca tttcagaatt tttgccgttc cactgatcaa gagtagcgct gttaccgaag
     2641 ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc tgcaccatta
     2701 ccgccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg cagagcaa
//
