LOCUS       sequence-197204-        9961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA vec.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, 
            Iacobuzio-Donahue CA, Massague J
  TITLE     TGF-beta Tumor Suppression through a Lethal EMT.
  JOURNAL   Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. 
            Epub 2016 Feb 18.
  PUBMED    26898331
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.009"; journalName: "Cell"; date: 
            "2016-02-25- 25"; volume: "164"; issue: "5"; pages: "1015-30"
COMMENT     Sequence Label: GEPIR Sox4.2091
FEATURES             Location/Qualifiers
     source          1..9961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      5..593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     494..513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     747..764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    881..902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(917..922,923..940,941..947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    955..971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     960..982
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     979..995
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     979..995
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1014..1034
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1016..1034
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1062..1288
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1289..1469
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1516..1641
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2134..2367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2552..2596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2863..2980
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2996..3374
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3004..3022
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3040..3058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3076..3094
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3112..3130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3148..3166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3184..3202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3220..3238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3283..3307
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3398..3400,3401..3403,3404..4117)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3443..3464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3704..3723)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4051..4072
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           4311..4325
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        4504..5003
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4539..4562)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4936..4955
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5024..5623
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5024..5043)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5520..5540
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5684..6251
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5701..6253
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5845..5862)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6072..6091
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6265..6882,6883..6888,6889..6924,6925..6927,6928..6963,
                     6964..6966,6967..7002,7003..7011)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7057..7645
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7110..7130)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7528..7539)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7852..8085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8157..8278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8194..8213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8248..8267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8318..8453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8473..8492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8474..8492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8502..8519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8502..8518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8511..8533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8660..9115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8747..8766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8957..8978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9141..9245
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9430..9808
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(9464..9483)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
       61 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      781 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      841 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      901 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      961 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     1021 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     1081 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     1141 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     1201 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     1261 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     1321 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1381 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1441 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1501 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1561 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1621 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1681 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1741 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1801 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1861 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1921 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1981 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     2041 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     2101 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     2161 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     2221 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     2281 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     2341 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2401 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2461 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2521 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2581 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2641 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2701 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2761 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2821 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
     2881 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2941 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgattcta gattcgagtt
     3001 tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac
     3061 gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca
     3121 gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt
     3181 tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac
     3241 gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg
     3301 tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt
     3361 cctaccctcg taaagtcgag cttgcgttgg atccaccatg gtgagcaagg gcgaggagct
     3421 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3481 cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3541 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3601 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3661 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3721 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3781 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3841 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat
     3901 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3961 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     4021 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4081 cgggatcact ctcggcatgg acgagctgta caagtaatga cgtacggcgg ccgcctcgac
     4141 tagggataac agggtaattg tttgaatgag gcttcagtac tttacagaat cgttgcctgc
     4201 acatcttgga aacacttgct gggattactt cgacttctta acccaacaga aggctcgaga
     4261 aggtatattg ctgttgacag tgagcgcaaa gaagaagaaa gaaagaaata gtgaagccac
     4321 agatgtattt ctttctttct tcttcttttt gcctactgcc tcggacttca aggggctaga
     4381 attcgagcaa ttatcttgtt tactaaaact gaataccttg ctatctcttt gatacatttt
     4441 tacaaagctg aattaaaatg gtataaatta aatcactttt ttcaattgac gcgtaattct
     4501 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     4561 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     4621 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     4681 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     4741 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     4801 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     4861 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     4921 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     4981 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     5041 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5101 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5161 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5221 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5281 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5341 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5401 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5461 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5521 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5581 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     5641 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcc aattccgccc ccccccccta
     5701 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5761 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5821 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5881 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5941 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     6001 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     6061 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     6121 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     6181 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6241 cacgatgata atatggccac aaccatgagt agactggaca agagcaaagt cataaacgga
     6301 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     6361 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     6421 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     6481 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     6541 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     6601 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     6661 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     6721 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     6781 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     6841 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     6901 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     6961 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta atccggactc
     7021 gacctgcagc caagcttatc gaaacgcgtc tggaacaatc aacctctgga ttacaaaatt
     7081 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7141 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7201 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7261 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7321 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7381 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7441 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7501 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7561 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7621 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     7681 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7741 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7801 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7861 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8101 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8161 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8221 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8281 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8401 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8461 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8521 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8581 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8641 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9061 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9241 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9301 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9361 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9421 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9481 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9541 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9601 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9661 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9721 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9781 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9841 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9901 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9961 g
//
