LOCUS       sequence-197205-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to AVPR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pAVPR2_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..574
                     /label=chicken beta-actin promoter
     intron          575..802
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             2115..2135
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2136..2156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2175..2201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2229..3209,3210..3212,3213..4727)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4731..4751
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4770..4919
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4947..5000
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5016..5402
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5457..5477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5493..6035
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             6060..6431
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(6459..6476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6465..6672
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6681..6821
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6681..6810
                     /label=AAV2 ITR
     rep_origin      6896..7351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6983..7002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7193..7214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7368..7387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7487..7509
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7547..7565)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7633..7737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7738..7806,7807..8598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7956..7975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8769..9357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9258..9277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      421 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      481 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      541 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      601 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      661 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc tgagcaagag
      721 gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc tggagcacct
      781 gcctgaaatc actttttttc aggttggacc ggtgcgccac catgaagacg atcatcgccc
      841 tgagctacat cttctgcctg gtattcgccg actacaagga cgatgatgac gccagcatcg
      901 atatgctcat ggcctctacc acaagcgctg tgcccggaca tccatctctt ccaagtcttc
      961 cctccaacag ctcacaggag aggcctctgg atacccggga cccacttctt gcaagagcgg
     1021 agctcgcctt gctctccatc gttttcgttg ccgtcgcgtt gagcaatggt ctggtgctgg
     1081 ccgccctggc gcgacgaggc cgccgaggcc actgggcgcc tattcatgtc tttatcggcc
     1141 atctctgtct tgcagacctg gccgtggcgc tgtttcaggt gctccctcaa ctggcttgga
     1201 aagccaccga tagattccgg gggccagacg cactgtgtcg ggccgtgaaa tacctgcaga
     1261 tggtgggaat gtatgccagt tcatatatga tacttgctat gaccttggac agacacaggg
     1321 ctatctgtcg ccccatgctg gcgtataggc acggttccgg agcccattgg aacaggccag
     1381 ttctggttgc gtgggctttt tccctgcttc tgagcttgcc ccagttgttt attttcgcac
     1441 agagaaatgt ggagggcgga tctggcgtga ccgattgctg ggcttgcttc gctgagccat
     1501 ggggcagaag gacctacgtg acgtggattg ctctcatggt gttcgttgct cccaccctgg
     1561 ggatcgccgc atgccaggtc ctgatattca gagaaataca cgcctccttg gttcctgggc
     1621 caagcgagcg ccctggtgga agaagacggg gtcgaaggac tggctctcca ggcgaaggcg
     1681 cccacgtatc agctgcggta gctaagaccg tacgcatgac actggtcatc gtcgtcgttt
     1741 acgttctttg ctgggccccg ttttttttgg tgcagctctg ggcggcctgg gaccccgaag
     1801 cacccctgga aggtgctcca ttcgttctgc tcatgcttct cgcttcactg aatagctgta
     1861 ccaatccttg gatttacgcc tctttctcct ccagtgttag ctccgagctg cggagtcttc
     1921 tctgttgcgc ccggggcagg acccccccaa gcctgggtcc acaggacgaa tcctgcacta
     1981 ccgcctccag ttccctggcc aaagacacct cttctatcga taccggtgga cgcaccccac
     2041 ccagcctggg tccccaagat gagtcctgca ccaccgccag ctcctccctg gccaaggaca
     2101 cttcatcgac cggtgagaac ctgtacttcc agggccccaa gaagaagagg aaggtggggg
     2161 gaggcagcac tagttaccca tacgacgtac cagattacgc tggaggttct ggatccggcg
     2221 gtggctcgaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
     2281 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
     2341 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
     2401 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
     2461 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
     2521 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
     2581 ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
     2641 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
     2701 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
     2761 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
     2821 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc
     2881 ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
     2941 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
     3001 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
     3061 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     3121 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
     3181 acaacaaggt gctgaccaga agcgacaagg cccggggcaa gagcgacaac gtgccctccg
     3241 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     3301 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     3361 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     3421 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     3481 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     3541 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
     3601 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     3661 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     3721 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     3781 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     3841 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     3901 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     3961 agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     4021 gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     4081 agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca
     4141 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     4201 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     4261 tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     4321 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     4381 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     4441 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     4501 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     4561 ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc
     4621 ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca
     4681 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacagc cccaagaaga
     4741 agagaaaggt ggaggccagc ggacgggctg acgcattgga cgattttgat ctggatatgc
     4801 tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat gcccttgatg
     4861 actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg gacatgctga
     4921 ttaacggtac cgctagcggc agtggagagg gcagaggaag tctgctaaca tgcggtgacg
     4981 tcgaggagaa tcctggccca gtgagcaagc ttatggcttc aaactttact cagttcgtgc
     5041 tcgtggacaa tggtgggaca ggggatgtga cagtggctcc ttctaatttc gctaatgggg
     5101 tggcagagtg gatcagctcc aactcacgga gccaggccta caaggtgaca tgcagcgtca
     5161 ggcagtctag tgcccagaag agaaagtata ccatcaaggt ggaggtcccc aaagtggcta
     5221 cccagacagt gggcggagtc gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg
     5281 agctcactat cccaattttc gctaccaatt ctgactgtga actcatcgtg aaggcaatgc
     5341 aggggctcct caaagacggt aatcctatcc cttccgccat cgccgctaac tcaggtatct
     5401 acagcgctgg aggaggtgga agcggaggag gaggaagcgg aggaggaggt agcggaccta
     5461 agaaaaagag gaaggtggcg gccgctggat ccccttcagg gcagatcagc aaccaggccc
     5521 tggctctggc ccctagctcc gctccagtgc tggcccagac tatggtgccc tctagtgcta
     5581 tggtgcctct ggcccagcca cctgctccag cccctgtgct gaccccagga ccaccccagt
     5641 cactgagcgc tccagtgccc aagtctacac aggccggcga ggggactctg agtgaagctc
     5701 tgctgcacct gcagttcgac gctgatgagg acctgggagc tctgctgggg aacagcaccg
     5761 atcccggagt gttcacagat ctggcctccg tggacaactc tgagtttcag cagctgctga
     5821 atcagggcgt gtccatgtct catagtacag ccgaaccaat gctgatggag taccccgaag
     5881 ccattacccg gctggtgacc ggcagccagc ggccccccga ccccgctcca actcccctgg
     5941 gaaccagcgg cctgcctaat gggctgtccg gagatgaaga cttctcaagc atcgctgata
     6001 tggactttag tgccctgctg tcacagattt cctctagtgg gcagggagga ggtggaagcg
     6061 gcttcagcgt ggacaccagt gccctgctgg acctgttcag cccctcggtg accgtgcccg
     6121 acatgagcct gcctgacctt gacagcagcc tggccagtat ccaagagctc ctgtctcccc
     6181 aggagccccc caggcctccc gaggcagaga acagcagccc ggattcaggg aagcagctgg
     6241 tgcactacac agcgcagccg ctgttcctgc tggaccccgg ctccgtggac accgggagca
     6301 acgacctgcc ggtgctgttt gagctgggag agggctccta cttctccgaa ggggacggct
     6361 tcgccgagga ccccaccatc tccctgctga caggctcgga gcctcccaaa gccaaggacc
     6421 ccactgtctc ctaagaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6481 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6541 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6601 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag
     6661 gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     6721 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     6781 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     6841 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     6901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6961 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7081 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     7141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7201 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     7261 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7321 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     7381 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7441 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7501 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     7561 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7621 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7681 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7741 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7801 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7861 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7921 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7981 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8041 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8101 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8161 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8221 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8281 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8341 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8401 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc
     8461 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8521 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8581 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8641 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8881 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatg
//
