LOCUS       sequence-197206-        9191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to LPAR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pLPAR1_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          9..236
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1528..1548
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1549..1569
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1588..1614
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1642..2622,2623..2625,2626..4140)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4144..4164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4183..4332
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4360..4413
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4429..4815
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4870..4890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4906..5448
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5473..5844
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(5872..5889)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5878..6085
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6094..6234
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6094..6223
                     /label=AAV2 ITR
     rep_origin      6309..6764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6396..6415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6606..6627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6781..6800)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6900..6922
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6960..6978)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7046..7150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7151..7219,7220..8011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7369..7388)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8182..8770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8671..8690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8842..9127
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
       61 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
      121 cttctcctcc gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg
      181 gtggggtatt aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg
      241 gaccggtgcg ccaccatgaa gacgatcatc gccctgagct acatcttctg cctggtattc
      301 gccgactaca aggacgatga tgacgccagc atcgatatgg ccgctattag caccagtatc
      361 ccagttatct ctcagcccca gtttacagcg atgaatgagc ctcagtgctt ttacaacgaa
      421 agcatcgcct ttttctataa cagatccggg aagcatctgg ccaccgaatg gaatacggtg
      481 tctaaattgg tcatgggcct tggtattacc gtttgcatct ttatcatgct tgcaaacttg
      541 ctggtgatgg tggctatcta cgtgaaccgc agattccatt ttccaattta ctacttgatg
      601 gcaaatcttg ccgcagctga cttcttcgcg gggttggcat atttctactt gatgtttaat
      661 accgggccca acacccgcag acttactgtc tcaacttggc tgttgcgcca gggactcatc
      721 gacacttccc tgactgcctc tgtggcaaac ctgttggcca tcgcaattga gagacacata
      781 acggtgttcc gaatgcaact tcatacacgg atgtcaaacc ggagggtggt ggtggtgatc
      841 gtggtgatct ggaccatggc tatcgttatg ggcgccattc ctagcgtggg ttggaattgc
      901 atttgcgaca tcgagaactg ttccaatatg gcacctctgt attctgacag ttatctggtt
      961 ttctgggcca tctttaatct cgtgacattt gtcgtgatgg tggtactgta tgctcacatc
     1021 ttcggatacg tgagacaacg cacaatgcgg atgtctcgac acagcagcgg acccagacgg
     1081 aatcgggaca caatgatgtc cctcctcaaa accgtcgtaa tcgtgttggg cgcatttatc
     1141 atttgttgga cccccgggct cgtactgctt ctgctcgacg tgtgttgtcc ccagtgtgac
     1201 gttcttgcct atgagaagtt ctttcttttg ttggccgaat tcaactccgc aatgaatcct
     1261 attatttact cttatcgaga taaagagatg tccgcaactt ttcggcagat cctctgctgc
     1321 cagaggagcg agaatcctac aggccccact gaaggttcag atcggtcagc ttcatccctc
     1381 aaccacacaa tcctggccgg tgtacactcc aacgatcatt ccgtggtcat cgataccggt
     1441 ggacgcaccc cacccagcct gggtccccaa gatgagtcct gcaccaccgc cagctcctcc
     1501 ctggccaagg acacttcatc gaccggtgag aacctgtact tccagggccc caagaagaag
     1561 aggaaggtgg ggggaggcag cactagttac ccatacgacg taccagatta cgctggaggt
     1621 tctggatccg gcggtggctc gaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     1681 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     1741 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     1801 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     1861 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     1921 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     1981 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     2041 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     2101 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     2161 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     2221 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     2281 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     2341 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     2401 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     2461 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     2521 gacatcaacc ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag
     2581 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca aggcccgggg caagagcgac
     2641 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     2701 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     2761 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     2821 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     2881 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     2941 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     3001 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     3061 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     3121 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     3181 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     3241 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     3301 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     3361 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     3421 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     3481 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     3541 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     3601 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     3661 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     3721 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     3781 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     3841 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     3901 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     3961 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     4021 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     4081 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac
     4141 agccccaaga agaagagaaa ggtggaggcc agcggacggg ctgacgcatt ggacgatttt
     4201 gatctggata tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg
     4261 gatgcccttg atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac
     4321 ctggacatgc tgattaacgg taccgctagc ggcagtggag agggcagagg aagtctgcta
     4381 acatgcggtg acgtcgagga gaatcctggc ccagtgagca agcttatggc ttcaaacttt
     4441 actcagttcg tgctcgtgga caatggtggg acaggggatg tgacagtggc tccttctaat
     4501 ttcgctaatg gggtggcaga gtggatcagc tccaactcac ggagccaggc ctacaaggtg
     4561 acatgcagcg tcaggcagtc tagtgcccag aagagaaagt ataccatcaa ggtggaggtc
     4621 cccaaagtgg ctacccagac agtgggcgga gtcgaactgc ctgtcgccgc ttggaggtcc
     4681 tacctgaaca tggagctcac tatcccaatt ttcgctacca attctgactg tgaactcatc
     4741 gtgaaggcaa tgcaggggct cctcaaagac ggtaatccta tcccttccgc catcgccgct
     4801 aactcaggta tctacagcgc tggaggaggt ggaagcggag gaggaggaag cggaggagga
     4861 ggtagcggac ctaagaaaaa gaggaaggtg gcggccgctg gatccccttc agggcagatc
     4921 agcaaccagg ccctggctct ggcccctagc tccgctccag tgctggccca gactatggtg
     4981 ccctctagtg ctatggtgcc tctggcccag ccacctgctc cagcccctgt gctgacccca
     5041 ggaccacccc agtcactgag cgctccagtg cccaagtcta cacaggccgg cgaggggact
     5101 ctgagtgaag ctctgctgca cctgcagttc gacgctgatg aggacctggg agctctgctg
     5161 gggaacagca ccgatcccgg agtgttcaca gatctggcct ccgtggacaa ctctgagttt
     5221 cagcagctgc tgaatcaggg cgtgtccatg tctcatagta cagccgaacc aatgctgatg
     5281 gagtaccccg aagccattac ccggctggtg accggcagcc agcggccccc cgaccccgct
     5341 ccaactcccc tgggaaccag cggcctgcct aatgggctgt ccggagatga agacttctca
     5401 agcatcgctg atatggactt tagtgccctg ctgtcacaga tttcctctag tgggcaggga
     5461 ggaggtggaa gcggcttcag cgtggacacc agtgccctgc tggacctgtt cagcccctcg
     5521 gtgaccgtgc ccgacatgag cctgcctgac cttgacagca gcctggccag tatccaagag
     5581 ctcctgtctc cccaggagcc ccccaggcct cccgaggcag agaacagcag cccggattca
     5641 gggaagcagc tggtgcacta cacagcgcag ccgctgttcc tgctggaccc cggctccgtg
     5701 gacaccggga gcaacgacct gccggtgctg tttgagctgg gagagggctc ctacttctcc
     5761 gaaggggacg gcttcgccga ggaccccacc atctccctgc tgacaggctc ggagcctccc
     5821 aaagccaagg accccactgt ctcctaagaa ttcctagagc tcgctgatca gcctcgactg
     5881 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5941 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6001 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6061 aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca
     6121 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     6181 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     6241 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     6301 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6361 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     6421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     6541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6601 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     6661 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     6721 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     6781 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6841 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6901 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     6961 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7141 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7201 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7261 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7321 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7381 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7441 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7501 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7561 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7621 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7681 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7741 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7801 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7861 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7921 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7981 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8041 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8101 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8161 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8221 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8281 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8341 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8401 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8461 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8521 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8641 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8701 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8761 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8821 tgctcacatg ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc
     8881 gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg
     8941 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9001 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca
     9061 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9121 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     9181 acccccaatt t
//
