LOCUS       sequence-197207-        9398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to LPAR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: 
            pLPAR1_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
FEATURES             Location/Qualifiers
     source          1..9398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..804
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             2096..2116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2117..2137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2156..2182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2210..3190,3191..3193,3194..4708)
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4712..4732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4751..4900
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4928..4981
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4997..5383
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5438..5458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5474..6016
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             6041..6412
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(6440..6457)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6446..6653
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6662..6802
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6662..6791
                     /label=AAV2 ITR
     rep_origin      6877..7332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6964..6983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7174..7195
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7349..7368)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7468..7490
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7528..7546)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7614..7718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7719..7787,7788..8579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7937..7956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8750..9338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9239..9258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
      721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
      781 ctgcctgaaa tcactttttt tcaggttgga ccggtgcgcc accatgaaga cgatcatcgc
      841 cctgagctac atcttctgcc tggtattcgc cgactacaag gacgatgatg acgccagcat
      901 cgatatggcc gctattagca ccagtatccc agttatctct cagccccagt ttacagcgat
      961 gaatgagcct cagtgctttt acaacgaaag catcgccttt ttctataaca gatccgggaa
     1021 gcatctggcc accgaatgga atacggtgtc taaattggtc atgggccttg gtattaccgt
     1081 ttgcatcttt atcatgcttg caaacttgct ggtgatggtg gctatctacg tgaaccgcag
     1141 attccatttt ccaatttact acttgatggc aaatcttgcc gcagctgact tcttcgcggg
     1201 gttggcatat ttctacttga tgtttaatac cgggcccaac acccgcagac ttactgtctc
     1261 aacttggctg ttgcgccagg gactcatcga cacttccctg actgcctctg tggcaaacct
     1321 gttggccatc gcaattgaga gacacataac ggtgttccga atgcaacttc atacacggat
     1381 gtcaaaccgg agggtggtgg tggtgatcgt ggtgatctgg accatggcta tcgttatggg
     1441 cgccattcct agcgtgggtt ggaattgcat ttgcgacatc gagaactgtt ccaatatggc
     1501 acctctgtat tctgacagtt atctggtttt ctgggccatc tttaatctcg tgacatttgt
     1561 cgtgatggtg gtactgtatg ctcacatctt cggatacgtg agacaacgca caatgcggat
     1621 gtctcgacac agcagcggac ccagacggaa tcgggacaca atgatgtccc tcctcaaaac
     1681 cgtcgtaatc gtgttgggcg catttatcat ttgttggacc cccgggctcg tactgcttct
     1741 gctcgacgtg tgttgtcccc agtgtgacgt tcttgcctat gagaagttct ttcttttgtt
     1801 ggccgaattc aactccgcaa tgaatcctat tatttactct tatcgagata aagagatgtc
     1861 cgcaactttt cggcagatcc tctgctgcca gaggagcgag aatcctacag gccccactga
     1921 aggttcagat cggtcagctt catccctcaa ccacacaatc ctggccggtg tacactccaa
     1981 cgatcattcc gtggtcatcg ataccggtgg acgcacccca cccagcctgg gtccccaaga
     2041 tgagtcctgc accaccgcca gctcctccct ggccaaggac acttcatcga ccggtgagaa
     2101 cctgtacttc cagggcccca agaagaagag gaaggtgggg ggaggcagca ctagttaccc
     2161 atacgacgta ccagattacg ctggaggttc tggatccggc ggtggctcga agcccgcctt
     2221 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     2281 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     2341 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     2401 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     2461 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     2521 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     2581 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     2641 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     2701 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     2761 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     2821 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     2881 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     2941 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     3001 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     3061 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     3121 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     3181 aagcgacaag gcccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     3241 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     3301 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     3361 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     3421 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     3481 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     3541 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     3601 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     3661 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     3721 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     3781 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     3841 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     3901 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     3961 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     4021 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     4081 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     4141 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     4201 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     4261 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     4321 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     4381 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     4441 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     4501 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     4561 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     4621 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     4681 gatcgacctg tctcagctgg gaggcgacag ccccaagaag aagagaaagg tggaggccag
     4741 cggacgggct gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga
     4801 tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct
     4861 cggcagtgac gcccttgatg atttcgacct ggacatgctg attaacggta ccgctagcgg
     4921 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     4981 agtgagcaag cttatggctt caaactttac tcagttcgtg ctcgtggaca atggtgggac
     5041 aggggatgtg acagtggctc cttctaattt cgctaatggg gtggcagagt ggatcagctc
     5101 caactcacgg agccaggcct acaaggtgac atgcagcgtc aggcagtcta gtgcccagaa
     5161 gagaaagtat accatcaagg tggaggtccc caaagtggct acccagacag tgggcggagt
     5221 cgaactgcct gtcgccgctt ggaggtccta cctgaacatg gagctcacta tcccaatttt
     5281 cgctaccaat tctgactgtg aactcatcgt gaaggcaatg caggggctcc tcaaagacgg
     5341 taatcctatc ccttccgcca tcgccgctaa ctcaggtatc tacagcgctg gaggaggtgg
     5401 aagcggagga ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc
     5461 ggccgctgga tccccttcag ggcagatcag caaccaggcc ctggctctgg cccctagctc
     5521 cgctccagtg ctggcccaga ctatggtgcc ctctagtgct atggtgcctc tggcccagcc
     5581 acctgctcca gcccctgtgc tgaccccagg accaccccag tcactgagcg ctccagtgcc
     5641 caagtctaca caggccggcg aggggactct gagtgaagct ctgctgcacc tgcagttcga
     5701 cgctgatgag gacctgggag ctctgctggg gaacagcacc gatcccggag tgttcacaga
     5761 tctggcctcc gtggacaact ctgagtttca gcagctgctg aatcagggcg tgtccatgtc
     5821 tcatagtaca gccgaaccaa tgctgatgga gtaccccgaa gccattaccc ggctggtgac
     5881 cggcagccag cggccccccg accccgctcc aactcccctg ggaaccagcg gcctgcctaa
     5941 tgggctgtcc ggagatgaag acttctcaag catcgctgat atggacttta gtgccctgct
     6001 gtcacagatt tcctctagtg ggcagggagg aggtggaagc ggcttcagcg tggacaccag
     6061 tgccctgctg gacctgttca gcccctcggt gaccgtgccc gacatgagcc tgcctgacct
     6121 tgacagcagc ctggccagta tccaagagct cctgtctccc caggagcccc ccaggcctcc
     6181 cgaggcagag aacagcagcc cggattcagg gaagcagctg gtgcactaca cagcgcagcc
     6241 gctgttcctg ctggaccccg gctccgtgga caccgggagc aacgacctgc cggtgctgtt
     6301 tgagctggga gagggctcct acttctccga aggggacggc ttcgccgagg accccaccat
     6361 ctccctgctg acaggctcgg agcctcccaa agccaaggac cccactgtct cctaagaatt
     6421 cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6481 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6541 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6601 gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgctgg ggagcggccg
     6661 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     6721 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     6781 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     6841 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     6901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     6961 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7021 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7081 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7141 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7201 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     7261 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7321 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7381 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7441 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7501 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7561 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7681 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7741 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7801 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7861 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7921 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7981 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8041 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8101 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8161 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8221 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8281 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8341 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8401 ttattgctga taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg
     8461 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8521 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8581 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8881 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9361 tacggttcct ggccttttgc tggccttttg ctcacatg
//
