LOCUS       sequence-197209-       13406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, 
            Iacobuzio-Donahue CA, Massague J
  TITLE     TGF-beta Tumor Suppression through a Lethal EMT.
  JOURNAL   Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. 
            Epub 2016 Feb 18.
  PUBMED    26898331
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.009"; journalName: "Cell"; date: 
            "2016-02-25- 25"; volume: "164"; issue: "5"; pages: "1015-30"
COMMENT     Sequence Label: pTripz Sox4.965
FEATURES             Location/Qualifiers
     source          1..13406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    98..116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    134..152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        235..273
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     270..294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             310..1005
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           1018..1139
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1167..1181
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1209..1335
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(1430..1448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1461..2672
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2509..2528
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(2685..2718)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             2725..3432
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(3433..3466)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    3489..4061
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3656..3673)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3883..3902
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4064..4666
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4563..4583
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4682..5270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4735..4755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5153..5164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5477..5710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5811..5828)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5817..6041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6087..6515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6174..6193)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6384..6405
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6524..6544)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6529..6858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6709..6844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6771..6790
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8066..8187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8236..8252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8236..8252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8249..8271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8260..8276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8284..8290,8291..8308,8309..8314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8329..8350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8467..8484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8638..9226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8718..8737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9397..10188,10189..10257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10020..10039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10258..10362)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10410..10544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             10712..11345
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11392..11517
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12014..12247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12432..12476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12774..12891
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(12956..13330)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      13327..13336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(13340..13358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(13350..13397)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 aggtccgagg ttctagacga gtttactccc tatcagtgat agagaacgat gtcgagttta
       61 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
      121 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
      181 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
      241 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcaccg
      301 gtcgccacca tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc
      361 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
      421 acccagacca tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
      481 ctggctacca gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc
      541 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa
      601 gacgggggcg tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac
      661 aacgtcaaga tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
      721 cgcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac
      781 agccagatgg ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca
      841 tacagatcca agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac
      901 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct
      961 gtggccaagt actgcgacct ccctagcaaa ctggggcaca gataatcgat agtttgtttg
     1021 aatgaggctt cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga
     1081 ttacttcttc aggttaaccc aacagaaggc tcgagaaggt atattgctgt tgacagtgag
     1141 cgccagcgac aagattccgt tcattagtga agccacagat gtaatgaacg gaatcttgtc
     1201 gctgttgcct actgcctcgg aattcaaggg gctactttag gagcaattat cttgtttact
     1261 aaaactgaat accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat
     1321 aaattaaatc acttttttca attggaagac taatgcggcc ggccattact ccgtctcgtg
     1381 tcttgttgca tatgtctgct ggtttgtttg atgttgtttg cgggcgggcc ctatagtgag
     1441 tcgtattacc taggacgcgt ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc
     1501 cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc
     1561 cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta
     1621 tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc
     1681 agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc
     1741 gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
     1801 gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt
     1861 gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg
     1921 gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga
     1981 actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac
     2041 gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga
     2101 acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg
     2161 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
     2221 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
     2281 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
     2341 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
     2401 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga
     2461 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
     2521 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
     2581 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta
     2641 aattctggcc gtttttggct tttttgttag acgctagcgg atccataact tcgtatagta
     2701 tacattatac gaagttatgc caccatgtct aggctggaca agagcaaagt cataaacgga
     2761 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     2821 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     2881 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     2941 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     3001 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     3061 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     3121 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     3181 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     3241 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     3301 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     3361 gacgattttg acttagacat gctcccagcc gatgcccttg acgattttga ccttgacatg
     3421 ctccccgggt aaataacttc gtatagtata cattatacga agttatggat ccgcggccgc
     3481 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     3541 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3601 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3661 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3721 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3781 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3841 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3901 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3961 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     4021 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggcca ccgagtacaa
     4081 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     4141 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     4201 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4261 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4321 ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc
     4381 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4441 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     4501 ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc
     4561 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     4621 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagttc gcgtctggaa
     4681 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4741 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4801 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4861 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4921 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4981 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5041 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     5101 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5161 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5221 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     5281 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     5341 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     5401 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5461 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5521 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5581 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5641 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5701 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5761 tgaatatcag agagtgagag gccttgacat tgtttaaacc cgctgatcag cctcgactgt
     5821 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5881 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5941 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6001 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6061 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6301 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6481 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6781 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6841 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     6901 cacgtgatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     6961 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     7021 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     7081 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     7141 gacattgggg aattaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     7201 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     7261 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     7321 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     7381 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     7441 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     7501 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     7561 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     7621 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     7681 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     7741 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     7801 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     7861 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     7921 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7981 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8041 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8101 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8161 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8221 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8281 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8341 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8401 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9541 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10261 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10321 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10381 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
    10441 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10501 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10561 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10621 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10681 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    10741 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    10801 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    10861 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    10921 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    10981 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11041 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11101 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11161 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11221 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11281 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11341 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11401 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11461 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    11521 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    11581 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    11641 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    11701 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    11761 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    11821 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
    11881 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    11941 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    12001 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    12061 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    12121 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    12181 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    12241 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    12301 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    12361 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    12421 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    12481 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    12541 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    12601 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
    12661 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
    12721 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
    12781 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    12841 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    12901 tacagggaca gcagagatcc agtttggtta gtaccgggcc cgctctagtc cggaatcagt
    12961 cctgctcctc ggccacgaag tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc
    13021 gcccccacgg ctgctcgccg atctcggtca tggccggccc ggaggcgtcc cggaagttcg
    13081 tggacacgac ctccgaccac tcggcgtaca gctcgtccag gccgcgcacc cacacccagg
    13141 ccagggtgtt gtccggcacc acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt
    13201 cccggaccac accggcgaag tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg
    13261 tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag caccggaacg gcactggtca
    13321 acttggccat ggtggccctc ctatagtgag tcgtattata ctatgccgat atactatgcc
    13381 gatgattaat tgtcaacacg tgctgc
//
