LOCUS       sequence-197217-        9119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Entingh-Pearsall A, Kahn CR
  TITLE     Differential roles of the insulin and insulin-like growth factor-I 
            (IGF-I) receptors in response to insulin and IGF-I.
  JOURNAL   J Biol Chem. 2004 Sep 3. 279(36):38016-24.
  PUBMED    15247278
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Sep 3. 279(36):38016-24."
COMMENT     Sequence Label: pBABE-bleo IGF-1R
FEATURES             Location/Qualifiers
     source          1..9119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5627..5647)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5632..5961
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5812..5947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6021..6395
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             6613..7082
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7212..7229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7383..7971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7463..7482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8131..8922,8923..8991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8754..8773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8992..9096)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acaattctgc agatatccat cacactggcg gccgctcgac cttttttttt tttttgagaa
     1441 aggggaattt catcccaaat aaaaggaatg aagtctggct ccggaggagg gtccccgacc
     1501 tcgctgtggg ggctcctgtt tctctccgcc gcgctctcgc tctggccgac gagtggagaa
     1561 atctgcgggc caggcatcga catccgcaac gactatcagc agctgaagcg cctggagaac
     1621 tgcacggtga tcgagggcta cctccacatc ctgctcatct ccaaggccga ggactaccgc
     1681 agctaccgct tccccaagct cacggtcatt accgagtact tgctgctgtt ccgagtggct
     1741 ggcctcgaga gcctcggaga cctcttcccc aacctcacgg tcatccgcgg ctggaaactc
     1801 ttctacaact acgccctggt catcttcgag atgaccaatc tcaaggatat tgggctttac
     1861 aacctgagga acattactcg gggggccatc aggattgaga aaaatgctga cctctgttac
     1921 ctctccactg tggactggtc cctgatcctg gatgcggtgt ccaataacta cattgtgggg
     1981 aataagcccc caaaggaatg tggggacctg tgtccaggga ccatggagga gaagccgatg
     2041 tgtgagaaga ccaccatcaa caatgagtac aactaccgct gctggaccac aaaccgctgc
     2101 cagaaaatgt gcccaagcac gtgtgggaag cgggcgtgca ccgagaacaa tgagtgctgc
     2161 caccccgagt gcctgggcag ctgcagcgcg cctgacaacg acacggcctg tgtagcttgc
     2221 cgccactact actatgccgg tgtctgtgtg cctgcctgcc cgcccaacac ctacaggttt
     2281 gagggctggc gctgtgtgga ccgtgacttc tgcgccaaca tcctcagcgc cgagagcagc
     2341 gactccgagg ggtttgtgat ccacgacggc gagtgcatgc aggagtgccc ctcgggcttc
     2401 atccgcaacg gcagccagag catgtactgc atcccttgtg aaggtccttg cccgaaggtc
     2461 tgtgaggaag aaaagaaaac aaagaccatt gattctgtta cttctgctca gatgctccaa
     2521 ggatgcacca tcttcaaggg caatttgctc attaacatcc gacgggggaa taacattgct
     2581 tcagagctgg agaacttcat ggggctcatc gaggtggtga cgggctacgt gaagatccgc
     2641 cattctcatg ccttggtctc cttgtccttc ctaaaaaacc ttcgcctcat cctaggagag
     2701 gagcagctag aagggaatta ctccttctac gtcctcgaca accagaactt gcagcaactg
     2761 tgggactggg accaccgcaa cctgaccatc aaagcaggga aaatgtactt tgctttcaat
     2821 cccaaattat gtgtttccga aatttaccgc atggaggaag tgacggggac taaagggcgc
     2881 caaagcaaag gggacataaa caccaggaac aacggggaga gagcctcctg tgaaagtgac
     2941 gtcctgcatt tcacctccac caccacgtcg aagaatcgca tcatcataac ctggcaccgg
     3001 taccggcccc ctgactacag ggatctcatc agcttcaccg tttactacaa ggaagcaccc
     3061 tttaagaatg tcacagagta tgatgggcag gatgcctgcg gctccaacag ctggaacatg
     3121 gtggacgtgg acctcccgcc caacaaggac gtggagcccg gcatcttact acatgggctg
     3181 aagccctgga ctcagtacgc cgtttacgtc aaggctgtga ccctcaccat ggtggagaac
     3241 gaccatatcc gtggggccaa gagtgagatc ttgtacattc gcaccaatgc ttcagttcct
     3301 tccattccct tggacgttct ttcagcatcg aactcctctt ctcagttaat cgtgaagtgg
     3361 aaccctccct ctctgcccaa cggcaacctg agttactaca ttgtgcgctg gcagcggcag
     3421 cctcaggacg gctaccttta ccggcacaat tactgctcca aagacaaaat ccccatcagg
     3481 aagtatgccg acggcaccat cgacattgag gaggtcacag agaaccccaa gactgaggtg
     3541 tgtggtgggg agaaagggcc ttgctgcgcc tgccccaaaa ctgaagccga gaagcaggcc
     3601 gagaaggagg aggctgaata ccgcaaagtc tttgagaatt tcctgcacaa ctccatcttc
     3661 gtgcccagac ctgaaaggaa gcggagagat gtcatgcaag tggccaacac caccatgtcc
     3721 agccgaagca ggaacaccac ggccgcagac acctacaaca tcactgaccc ggaagagctg
     3781 gagacagagt accctttctt tgagagcaga gtggataaca aggagagaac tgtcatttct
     3841 aaccttcggc ctttcacatt gtaccgcatc gatatccaca gctgcaacca cgaggctgag
     3901 aagctgggct gcagcgcctc caacttcgtc tttgcaagga ctatgcccgc agaaggagca
     3961 gatgacattc ctgggccagt gacctgggag ccaaggcctg aaaactccat ctttttaaag
     4021 tggccggaac ctgagaatcc caatggattg attctaatgt atgaaataaa atacggatca
     4081 caagttgagg atcagcgaga atgtgtgtcc agacaggaat acaggaagta tggaggggcc
     4141 aagctaaacc ggctaaaccc ggggaactac acagcccgga ttcaggccac atctctctct
     4201 gggaatgggt cgtggacaga tcctgtgttc ttctatgtcc aggccaaaac aggatatgaa
     4261 aacttcatcc atctgatcat cgctctgccc gtcgctgtcc tgttgatcgt gggagggttg
     4321 gtgattatgc tgtacgtctt ccatagaaag agaaataaca gcaggctggg gaatggagtg
     4381 ctgtatgcct ctgtgaaccc ggagtacttc agcgctgctg atgtgtacgt tcctgatgag
     4441 tgggaggtgg ctcgggagaa gatcaccatg agccgggaac ttgggcaggg gtcgtttggg
     4501 atggtctatg aaggagttgc caagggtgtg gtgaaagatg aacctgaaac cagagtggcc
     4561 attaaaacag tgaacgaggc cgcaagcatg cgtgagagga ttgagtttct caacgaagct
     4621 tctgtgatga aggagttcaa ttgtcaccat gtggtgcgat tgctgggtgt ggtgtcccaa
     4681 ggccagccaa cactggtcat catggaactg atgacacggg gcgatctcaa aagttatctc
     4741 cggtctctga ggccagaaat ggagaataat ccagtcctag cacctccaag cctgagcaag
     4801 atgattcaga tggccggaga gattgcagac ggcatggcat acctcaacgc caataagttc
     4861 gtccacagag accttgctgc ccggaattgc atggtagccg aagatttcac agtcaaaatc
     4921 ggagattttg gtatgacgcg agatatctat gagacagact attaccggaa aggagggaaa
     4981 gggctgctgc ccgtgcgctg gatgtctcct gagtccctca aggatggagt cttcaccact
     5041 tactcggacg tctggtcctt cggggtcgtc ctctgggaga tcgccacact ggccgagcag
     5101 ccctaccagg gcttgtccaa cgagcaagtc cttcgcttcg tcatggaggg cggccttctg
     5161 gacaagccag acaactgtcc tgacatgctg tttgaactga tgcgcatgtg ctggcagtat
     5221 aaccccaaga tgaggccttc cttcctggag atcatcagca gcatcaaaga ggagatggag
     5281 cctggcttcc gggaggtctc cttctactac agcgaggaga acaagctgcc cgagccggag
     5341 gagctggacc tggagccaga gaacatggag agcgtccccc tggacccctc ggcctcctcg
     5401 tcctccctgc cactgcccga cagacactca ggacacaagg ccgagaacgg ccccggccct
     5461 ggggtgctgg tcctccgcgc cagcttcgac gagagacagc cttacgccca catgaacggg
     5521 ggccgcaaga acgagcgggc cttgccgctg ccccagtctt cgacctgctg atccttggat
     5581 ccactagttc taggtaggaa ttcgccagca cagtggtcga ccctgtggaa tgtgtgtcag
     5641 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5701 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5761 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5821 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5881 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5941 ggaggcctag gcttttgcaa aaagcttgcc agctggggcg ccctctggta aggttgggaa
     6001 gccctgcaga tctgatcacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     6061 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     6121 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     6181 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     6241 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     6301 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     6361 actgcgtgca cttcgtggcc gaggagcagg actgaccgac gccgaccaac accgccggtc
     6421 cgacgcggcc cgacgggtcc gaggcatcga taaaataaaa gattttattt agtctccaga
     6481 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     6541 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     6601 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     6661 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     6721 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     6781 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6841 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6901 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     6961 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     7021 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     7081 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     7141 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7801 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca aatcaatcta
     8101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
