LOCUS       sequence-197218-       11654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LEFTY2 gRNA used for targeted demethylation is inserted into the 
            WPXL lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V,
            Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, 
            Huangfu D
  TITLE     TET proteins safeguard bivalent promoters from de novo methylation 
            in human embryonic stem cells.
  JOURNAL   Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. 
            Epub 2017 Dec 4.
  PUBMED    29203910
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-017-0002-y"; journalName: "Nat Genet"; date: 
            "2018-01"; volume: "50"; issue: "1"; pages: "83-95"
COMMENT     Sequence Label: WPXL LEFTY2 gRNA
FEATURES             Location/Qualifiers
     source          1..11654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3352..3399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3418..4443
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3418..4443
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4557..4678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4707..4824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4914..4938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4955..5203
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4955..4975
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5126..5145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5224..5299
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5417..5441)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5485..6073
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5538..5558)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5956..5967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6217..6250)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6270..6450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6475..6493)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6476..6493)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6783..6802)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7016..7035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7135..7157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7195..7213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7281..7385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7386..7454,7455..8246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7604..7623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8417..9005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8906..8925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9159..9176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9246..9266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9251..9580
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9431..9566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10714..10779
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10909..10929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11354..11488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tgttgacaat
     3361 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     3421 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     3481 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     3541 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     3601 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     3661 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     3721 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     3781 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     3841 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     3901 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     3961 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     4021 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     4081 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     4141 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     4201 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     4261 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     4321 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     4381 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     4441 tagtctagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4501 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4561 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4621 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatt
     4681 aattaatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     4741 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4801 caaattacaa aaattcaaaa ttttatcgat cacgagacta gcctcgaggt ttaaactacg
     4861 ggatccaggc ctaagcttac gcgtcccagt gtggtggaat tctgcagata tcaacaagtt
     4921 tgtacaaaaa agcaggctcc gcggtggcgg ccgcgagggc ctatttccca tgattccttc
     4981 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     5041 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     5101 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     5161 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgagaagc agagcaggaa
     5221 gtcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     5281 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc
     5341 tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag gtacgaattc
     5401 aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggt
     5461 cgacgataaa actagtcata tgataatcaa cctctggatt acaaaatttg tgaaagattg
     5521 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5581 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5641 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5701 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5761 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5821 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5881 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5941 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6001 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6061 gccgcctccc cgcatcggta cgtatggcca ggtaccttta agaccaatga cttacaaggc
     6121 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6181 ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag catacattat
     6241 acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6301 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6361 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6421 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     6481 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     6541 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     6601 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     6661 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     6721 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     6781 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     6841 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     6901 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     6961 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     7021 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     7081 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     7141 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     7201 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7621 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8101 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9241 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9301 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9421 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9481 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9541 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     9601 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     9661 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     9721 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     9781 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     9841 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     9901 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     9961 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
    10021 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
    10081 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
    10141 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
    10201 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
    10261 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
    10321 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
    10381 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
    10441 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
    10501 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
    10561 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
    10621 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
    10681 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
    10741 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
    10801 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
    10861 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
    10921 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
    10981 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    11041 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
    11101 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
    11161 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    11221 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    11281 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    11341 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11461 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    11521 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    11581 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    11641 tacaaactta gtag
//
