LOCUS       sequence-197219-       11579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control
            of the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Nelson EV, Pacheco JR, Hume AJ, Cressey TN, Deflube LR, Ruedas JB, 
            Connor JH, Ebihara H, Muhlberger E
  TITLE     An RNA polymerase II-driven Ebola virus minigenome system as an 
            advanced tool for antiviral drug screening.
  JOURNAL   Antiviral Res. 2017 Aug 12;146:21-27. doi: 
            10.1016/j.antiviral.2017.08.005.
  PUBMED    28807685
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antiviral 
            Res."; date: "2017-08-12"; volume: "146:21-27. doi"; pages: " 
            10.1016/j.antiviral.2017.08.005"
COMMENT     Sequence Label: pCAGGS_L_EBOV
FEATURES             Location/Qualifiers
     source          1..11579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(8602..8621)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8667..8722
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8721..8740)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9082..9098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9082..9098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9095..9117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9106..9122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9130..9136,9137..9154,9155..9160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9175..9196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9255..9450
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9301..9436
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9456..9590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9493..9512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9547..9566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9658..9675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9829..10417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9909..9928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10588..11379,11380..11448))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11211..11230
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11449..11553)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcaag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcagctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagggtacc
     1741 cccggggcgg ccgcaaatgg ctacacaaca tacccaatac ccagacgcta ggttatcatc
     1801 accaattgta ttggaccaat gtgacctagt cactagagct tgcgggttat attcatcata
     1861 ctcccttaat ccgcaactac gcaactgtaa actcccgaaa catatctacc gtttgaaata
     1921 cgatgtaact gttaccaagt tcttgagtga tgtaccagtg gcgacattgc ccatagattt
     1981 catagtccca gttcttctca aggcactgtc aggcaatgga ttctgtcctg ttgagccgcg
     2041 gtgccaacag ttcttagatg aaatcattaa gtacacaatg caagatgctc tcttcttgaa
     2101 atattatctc aaaaatgtgg gtgctcaaga agactgtgtt gatgaacact ttcaagagaa
     2161 aatcttatct tcaattcagg gcaatgaatt tttacatcaa atgtttttct ggtatgatct
     2221 ggctatttta actcgaaggg gtagattaaa tcgaggaaac tctagatcaa catggtttgt
     2281 tcatgatgat ttaatagaca tcttaggcta tggggactat gttttttgga agatcccaat
     2341 ttcaatgtta ccactgaaca cacaaggaat cccccatgct gctatggact ggtatcaggc
     2401 atcagtattc aaagaagcgg ttcaagggca tacacacatt gtttctgttt ctactgccga
     2461 cgtcttgata atgtgcaaag atttaattac atgtcgattc aacacaactc taatctcaaa
     2521 aatagcagag attgaggatc cagtttgttc tgattatccc aattttaaga ttgtgtctat
     2581 gctttaccag agcggagatt acttactctc catattaggg tctgatgggt ataaaattat
     2641 taagttcctc gaaccattgt gcttggccaa aattcaatta tgctcaaagt acactgagag
     2701 gaagggccga ttcttaacac aaatgcattt agctgtaaat cacaccctag aagaaattac
     2761 agaaatgcgt gcactaaagc cttcacaggc tcaaaagatc cgtgaattcc atagaacatt
     2821 gataaggctg gagatgacgc cacaacaact ttgtgagcta ttttccattc aaaaacactg
     2881 ggggcatcct gtgctacata gtgaaacagc aatccaaaaa gttaaaaaac atgctacggt
     2941 gctaaaagca ttacgcccta tagtgatttt cgagacatac tgtgttttta aatatagtat
     3001 tgccaaacat tattttgata gtcaaggatc ttggtacagt gttacttcag ataggaatct
     3061 aacaccgggt cttaattctt atatcaaaag aaatcaattc cctccgttgc caatgattaa
     3121 agaactacta tgggaatttt accaccttga ccaccctcca cttttctcaa ccaaaattat
     3181 tagtgactta agtattttta taaaagacag agctaccgca gtagaaagga catgctggga
     3241 tgcagtattc gagcctaatg ttctaggata taatccacct cacaaattta gtactaaacg
     3301 tgtaccggaa caatttttag agcaagaaaa cttttctatt gagaatgttc tttcctacgc
     3361 acaaaaactc gagtatctac taccacaata tcggaacttt tctttctcat tgaaagagaa
     3421 agagttgaat gtaggtagaa ccttcggaaa attgccttat ccgactcgca atgttcaaac
     3481 actttgtgaa gctctgttag ctgatggtct tgctaaagca tttcctagca atatgatggt
     3541 agttacggaa cgtgagcaaa aagaaagctt attgcatcaa gcatcatggc accacacaag
     3601 tgatgatttt ggtgaacatg ccacagttag agggagtagc tttgtaactg atttagagaa
     3661 atacaatctt gcatttagat atgagtttac agcacctttt atagaatatt gcaaccgttg
     3721 ctatggtgtt aagaatgttt ttaattggat gcattataca atcccacagt gttatatgca
     3781 tgtcagtgat tattataatc caccacataa cctcacactg gagaatcgag acaacccccc
     3841 cgaagggcct agttcataca ggggtcatat gggagggatt gaaggactgc aacaaaaact
     3901 ctggacaagt atttcatgtg ctcaaatttc tttagttgaa attaagactg gttttaagtt
     3961 acgctcagct gtgatgggtg acaatcagtg cattactgtt ttatcagtct tccccttaga
     4021 gactgacgca gacgagcagg aacagagcgc cgaagacaat gcagcgaggg tggccgccag
     4081 cctagcaaaa gttacaagtg cctgtggaat ctttttaaaa cctgatgaaa catttgtaca
     4141 ttcaggtttt atctattttg gaaaaaaaca atatttgaat ggggtccaat tgcctcagtc
     4201 ccttaaaacg gctgcaagaa tggcaccatt gtctgatgca atttttgatg atcttcaagg
     4261 gaccctggct agtataggca ctgcttttga gcgatccatc tctgagacac gacatatctt
     4321 tccttgcagg ataaccgcag ctttccatac gtttttttcg gtgagaatct tgcaatatca
     4381 tcatctcggg ttcaataaag gttttgacct tggacagtta acactcggca aacctctgga
     4441 tttcggaaca atatcattgg cactagcggt accgcaggtg cttggagggt tatccttctt
     4501 gaatcctgag aaatgtttct accggaatct aggagatcca gttacctcag gcttattcca
     4561 gttaaaaact tatctccgaa tgattgagat ggatgattta ttcttacctt taattgcgaa
     4621 gaaccctggg aactgcactg ccattgactt tgtgctaaat cctagcggat taaatgtccc
     4681 tgggtcgcaa gacttaactt catttctgcg ccagattgta cgcaggacca tcaccctaag
     4741 tgcgaaaaac aaacttatta ataccttatt tcatgcgtca gctgacttcg aagacgaaat
     4801 ggtttgtaaa tggctattat catcaactcc tgttatgagt cgttttgcgg ccgatatctt
     4861 ttcacgcacg ccgagcggga agcgattgca aattctagga tacctggaag gaacacgcac
     4921 attattagcc tctaagatca tcaacaataa tacagagaca ccggttttgg acagactgag
     4981 gaaaataaca ttgcaaaggt ggagcctatg gtttagttat cttgatcatt gtgataatat
     5041 cctggcggag gctttaaccc aaataacttg cacagttgat ttagcacaga ttctgaggga
     5101 atattcatgg gctcatattt tagagggaag acctcttatt ggagccacac tcccatgtat
     5161 gattgagcaa ttcaaagtgt tttggctgaa accctacgaa caatgtccgc agtgttcaaa
     5221 tgcaaagcaa ccaggtggga aaccattcgt gtcagtggca gtcaagaaac atattgttag
     5281 tgcatggccg aacgcatccc gaataagctg gactatcggg gatggaatcc catacattgg
     5341 atcaaggaca gaagataaga taggacaacc tgctattaaa ccaaaatgtc cttccgcagc
     5401 cttaagagag gccattgaat tggcgtcccg tttaacatgg gtaactcaag gcagttcgaa
     5461 cagtgacttg ctaataaaac catttttgga agcacgagta aatttaagtg ttcaagaaat
     5521 acttcaaatg accccttcac attactcagg aaatattgtt cacaggtaca acgatcaata
     5581 cagtcctcat tctttcatgg ccaatcgtat gagtaattca gcaacgcgat tgattgtttc
     5641 tacaaacact ttaggtgagt tttcaggagg tggccagtct gcacgcgaca gcaatattat
     5701 tttccagaat gttataaatt atgcagttgc actgttcgat attaaattta gaaacactga
     5761 ggctacagat atccaatata atcgtgctca ccttcatcta actaagtgtt gcacccggga
     5821 agtaccagct cagtatttaa catacacatc tacattggat ttagatttaa caagataccg
     5881 agaaaacgaa ttgatttatg acagtaatcc tctaaaagga ggactcaatt gcaatatctc
     5941 attcgataat ccatttttcc aaggtaaacg gctgaacatt atagaagatg atcttattcg
     6001 actgcctcac ttatctggat gggagctagc caagaccatc atgcaatcaa ttatttcaga
     6061 tagcaacaat tcatctacag acccaattag cagtggagaa acaagatcat tcactaccca
     6121 tttcttaact tatcccaaga taggacttct gtacagtttt ggggcctttg taagttatta
     6181 tcttggcaat acaattcttc ggactaagaa attaacactt gacaattttt tatattactt
     6241 aactactcaa attcataatc taccacatcg ctcattgcga atacttaagc caacattcaa
     6301 acatgcaagc gttatgtcac ggttaatgag tattgatcct catttttcta tttacatagg
     6361 cggtgctgca ggtgacagag gactctcaga tgcggccagg ttatttttga gaacgtccat
     6421 ttcatctttt cttacatttg taaaagaatg gataattaat cgcggaacaa ttgtcccttt
     6481 atggatagta tatccgctag agggtcaaaa cccaacacct gtgaataatt ttctctatca
     6541 gatcgtagaa ctgctggtgc atgattcatc aagacaacag gcttttaaaa ctaccataag
     6601 tgatcatgta catcctcacg acaatcttgt ttacacatgt aagagtacag ccagcaattt
     6661 cttccatgca tcattggcgt actggaggag cagacacaga aacagcaacc gaaaatactt
     6721 ggcaagagac tcttcaactg gatcaagcac aaacaacagt gatggtcata ttgagagaag
     6781 tcaagaacaa accaccagag atccacatga tggcactgaa cggaatctag tcctacaaat
     6841 gagccatgaa ataaaaagaa cgacaattcc acaagaaaac acgcaccagg gtccgtcgtt
     6901 ccagtccttt ctaagtgact ctgcttgtgg tacagcaaat ccaaaactaa atttcgatcg
     6961 atcgagacac aatgtgaaat ttcaggatca taactcggca tccaagaggg aaggtcatca
     7021 aataatctca caccgtctag tcctaccttt ctttacatta tctcaaggga cacgccaatt
     7081 aacgtcatcc aatgagtcac aaacccaaga cgagatatca aagtacttac ggcaattgag
     7141 atccgtcatt gataccacag tttattgtag atttaccggt atagtctcgt ccatgcatta
     7201 caaacttgat gaggtccttt gggaaataga gagtttcaag tcggctgtga cgctagcaga
     7261 gggagaaggt gctggtgcct tactattgat tcagaaatac caagttaaga ccttattttt
     7321 caacacgcta gctactgagt ccagtataga gtcagaaata gtatcaggaa tgactactcc
     7381 taggatgctt ctacctgtta tgtcaaaatt ccataatgac caaattgaga ttattcttaa
     7441 caactcagca agccaaataa cagacataac aaatcctact tggtttaaag accaaagagc
     7501 aaggctacct aagcaagtcg aggttataac catggatgca gagacaacag agaatataaa
     7561 cagatcgaaa ttgtacgaag ctgtatataa attgatctta caccatattg atcctagcgt
     7621 attgaaagca gtggtcctta aagtctttct aagtgatact gagggtatgt tatggctaaa
     7681 tgataattta gccccgtttt ttgccactgg ttatttaatt aagccaataa cgtcaagtgc
     7741 tagatctagt gagtggtatc tttgtctgac gaacttctta tcaactacac gtaagatgcc
     7801 acaccaaaac catctcagtt gtaaacaggt aatacttacg gcattgcaac tgcaaattca
     7861 acgaagccca tactggctaa gtcatttaac tcagtatgct gactgtgagt tacatttaag
     7921 ttatatccgc cttggttttc catcattaga gaaagtacta taccacaggt ataacctcgt
     7981 cgattcaaaa agaggtccac tagtctctat cactcagcac ttagcacatc ttagagcaga
     8041 gattcgagaa ttaactaatg attataatca acagcgacaa agtcggactc aaacatatca
     8101 ctttattcgt actgcaaaag gacgaatcac aaaactagtc aatgattatt taaaattctt
     8161 tcttattgtg caagcattaa aacataatgg gacatggcaa gctgagttta agaaattacc
     8221 agagttgatt agtgtgtgca ataggttcta ccatattaga gattgcaatt gtgaagaacg
     8281 tttcttagtt caaaccttat atttacatag aatgcaggat tctgaagtta agcttatcga
     8341 aaggctgaca gggcttctga gtttatttcc ggatggtctc tacaggtttg attgaattac
     8401 cgagcatagt atcctgatac ttgcaaaggt tggttattaa catacagatt ataaaaaact
     8461 cataaattgc tctcatacat catattgatc taatctcaat aaacaactat ttaaataacg
     8521 aaaggagtcc ctatattata tactatattt agcctctctc cctgcgtgat aatcaaaaag
     8581 agctcttaat tagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     8641 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     8701 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     8761 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     8821 caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     8881 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     8941 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     9001 tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct cgacctgcag
     9061 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9121 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9181 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9241 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     9301 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9361 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9421 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     9481 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9541 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     9601 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10021 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10261 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10501 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10561 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10621 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10681 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10741 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10801 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10861 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10921 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10981 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11041 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11101 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11161 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11221 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11281 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11341 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11401 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11461 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11521 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
