LOCUS       sequence-19722-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Li B, de Lange T
  TITLE     Rap1 affects the length and heterogeneity of human telomeres.
  JOURNAL   Mol Biol Cell. 2003 Dec . 14(12):5060-8.
  PUBMED    14565979
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2003 Dec . 14(12):5060-8."
COMMENT     Sequence Label: pLPC FLAG hRap1 delta MC
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc tgccgccacc atggactaca
      121 aagacgatga cgataaagga tctgcggagg cgttggattt gggcaaagac cccaacgggc
      181 ccacccattc ctcgactctg ttcgtgaggg acgacggcag ctccatgtcc ttctacgtgc
      241 ggcccagccc ggccaagcgt cggctgtcga cgctcatcct gcacggcggc ggcaccgtgt
      301 gccgagtgca ggagcccggg gccgtgctgc tggcccagcc cggggaggcg ctggccgagg
      361 cctcgggtga tttcatctcc acgcagtaca tcctggactg cgtggagcgc aacgagaggc
      421 tggagctgga ggcctatcgg ctgggccccg cctcggcggc ggacaccggc tcggaagcaa
      481 agcccggggc cctggccgag ggcgccgcgg agccggagcc gcagcggcac gccggatccg
      541 tggtggatga gagccctcct gattttgaaa tacatataac tatgtgtgat gatgatccac
      601 ccacacctga ggaagactca gaaacacagc ctgatgagga ggaagaagaa gaagaagaaa
      661 aagtttctca accagaggtg ggagctgcca ttaagatcat tcggcagtta atg
//
