LOCUS       sequence-197226-       11457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression ,was generatedby recombining bothIRES an 
            HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin 
            shRNA/mEcad-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, 
            Hamilton NA, Neufeld Z, Yap AS
  TITLE     Cortical F-actin stabilization generates apical-lateral patterns of 
            junctional contractility that integrate cells into epithelia.
  JOURNAL   Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 
            2014 Jan 12.
  PUBMED    24413434
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2900"; 
            journalName: "Nat Cell Biol"; date: "2014-02"; volume: "16"; issue: 
            "2"; pages: "167-78"
COMMENT     Sequence Label: pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA...
FEATURES             Location/Qualifiers
     source          1..11457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        29..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        410..608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             626..806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    853..978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1475..1708
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1893..1937
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2235..2352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2407..2719
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2638..2658
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2776..2792
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2834..2867)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6098..6107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6104..6106,6107..6109,6110..6823)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6104..6106,6107..6109,6110..6820)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6149..6170)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6410..6429)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6757..6778
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6839..7412
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6862..7414
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7006..7023)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7233..7252
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7426..7452
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(8050..8083)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8139..8725
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8192..8212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8610..8621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8728..8744)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9254..9434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9496..10084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9576..9595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10255..11046,11047..11115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10878..10897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11116..11220)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11295..11314)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      781 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      841 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      901 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      961 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1021 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1081 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1141 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1201 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1261 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1321 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1381 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1441 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1501 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1561 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1621 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1681 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1741 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1801 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1861 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1921 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1981 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     2041 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2101 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2161 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     2221 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     2281 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2341 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgcgc
     2401 tctagagatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg
     2461 agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct agtaactata
     2521 gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta
     2581 catgataggc ttggatttct ataagagata caaatactaa attattattt taaaaaacag
     2641 cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact ataaatatcc
     2701 cttggagaaa agccttgttt gtcagctact ctagttctgt tcaagagaca gaactagagt
     2761 agctgacttt tttctcgagg tcgacggtat cgataagctc gcttcacgag attccagcag
     2821 gtcgagggac ctaataactt cgtatagcat acattatacg aagttatatt aagggttcca
     2881 agcttaagcg gccgctgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     2941 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     3001 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     3061 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     3121 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     3181 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     3241 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     3301 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
     3361 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatc gaattctgca
     3421 gtcgacggta ccgcggatgg gagcccggtg ccgcagcttt tccgcgctcc tgctcctgct
     3481 gcaggtctcc tcatggcttt gccaggagct ggagcctgag tcctgcagtc ccggcttcag
     3541 ttccgaggtc tacaccttcc cggtgccgga gaggcacctg gagagaggcc atgtcctggg
     3601 cagagtgaga tttgaaggat gcaccggccg gccaaggaca gccttctttt cggaagactc
     3661 ccgattcaaa gtggcgacag acggcaccat cacagtgaag cggcatctaa agctccacaa
     3721 gctggagacc agtttcctcg tccgcgcccg ggactccagt catagggagc tgtctaccaa
     3781 agtgacgctg aagtccatgg ggcaccacca tcaccggcac caccaccgcg accctgcctc
     3841 tgaatccaac ccagagctgc tcatgtttcc cagcgtgtac ccaggtctca gaagacagaa
     3901 acgagactgg gtcatccctc ccatcagctg ccccgaaaat gaaaagggtg aattcccaaa
     3961 gaacctggtt cagatcaaat ccaacaggga caaagaaaca aaggttttct acagcatcac
     4021 cggccaagga gctgacaaac cccccgttgg cgttttcatc attgagaggg agacaggctg
     4081 gctgaaagtg acacagcctc tggatagaga agccattgcc aagtacatcc tctattctca
     4141 tgccgtgtca tcaaatgggg aagcggtgga ggatcccatg gagatagtga tcacagtgac
     4201 agatcagaat gacaacaggc cagagtttac ccaggaggtg tttgagggat ccgttgcaga
     4261 aggcgctgtt ccaggaacct ccgtgatgaa ggtctcagcc accgatgcag acgatgacgt
     4321 caacacctac aacgctgcca tcgcctacac catcgtcagc caggatcctg agctgcctca
     4381 caaaaacatg ttcactgtca atagggacac cggggtcatc agtgtgctca cctctgggct
     4441 ggaccgagag agttacccta catacactct ggtggttcag gctgctgacc ttcaaggcga
     4501 aggcttgagc acaacagcca aggctgtgat cactgtcaag gatattaatg acaacgctcc
     4561 tgtcttcaac ccgagcacgt atcagggtca agtgcctgag aatgaggtca atgcccggat
     4621 cgccacactc aaagtgaccg atgatgatgc ccccaacact ccggcgtgga aagctgtgta
     4681 caccgtagtc aacgatcctg accagcagtt cgttgtcgtc acagacccca cgaccaatga
     4741 tggcattttg aaaacagcca agggcttgga ttttgaggcc aagcagcaat acatccttca
     4801 tgtgagagtg gagaacgagg aaccctttga ggggtctctt gtcccttcca cagccactgt
     4861 cactgtggac gtggtagacg tgaatgaagc ccccatcttt atgcctgcgg agaggagagt
     4921 cgaagtgccc gaagactttg gtgtgggtca ggaaatcaca tcttataccg ctcgagagcc
     4981 ggacacgttc atggatcaga agatcacgta tcggatttgg agggacactg ccaactggct
     5041 ggagattaac ccagagactg gtgccatttt cacgcgcgct gagatggaca gagaagacgc
     5101 tgagcatgtg aagaacagca catatgtagc tctcatcatc gccacagatg atggttcacc
     5161 cattgccact ggcacgggca ctcttctcct ggtcctgtta gacgtcaatg acaacgctcc
     5221 catcccagaa cctcgaaaca tgcagttctg ccagaggaac ccacagcctc atatcatcac
     5281 catcttggat ccagaccttc cccccaacac gtcccccttt actgctgagc taacccatgg
     5341 ggccagcgtc aactggacca ttgagtataa tgacgcagct caagaatctc tcattttgca
     5401 accaagaaag gacttagaga ttggcgaata caaaatccat ctcaagctcg cggataacca
     5461 gaacaaagac caggtgacca cgttggacgt ccatgtgtgt gactgtgaag ggacggtcaa
     5521 caactgcatg aaggcgggaa tcgtggcagc aggattgcaa gttcctgcca tcctcggaat
     5581 ccttggaggg atcctcgccc tgctgattct gatcctgctg ctcctactgt ttctacggag
     5641 gagaacggtg gtcaaagagc ccctgctgcc accagatgat gatacccggg acaatgtgta
     5701 ttactatgat gaagaaggag gtggagaaga agaccaggac tttgatttga gccagctgca
     5761 caggggcctg gatgcccgac cggaagtgac tcgaaatgat gtggctccca ccctcatgag
     5821 cgtgccccag tatcgtcccc gtcctgccaa tcctgatgaa attggaaact tcatcgatga
     5881 aaacctgaag gcagccgaca gcgaccccac ggcaccccct tacgactctc tgttggtgtt
     5941 cgattacgag ggcagtggtt ctgaagccgc tagcctgagc tcactgaact cctctgagtc
     6001 ggatcaggac caggactacg attatctgaa cgagtggggc aaccgattca agaagctggc
     6061 ggacatgtac ggcggtggcg aggacgacaa accggtcgcc accatggtga gcaagggcga
     6121 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6181 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6241 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6301 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6361 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6421 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6481 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6541 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6601 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6661 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6721 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6781 cgccgccggg atcactctcg gcatggacga gctgtacaag taacctgcag gggatccgcc
     6841 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     6901 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     6961 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     7021 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     7081 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     7141 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     7201 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     7261 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
     7321 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     7381 acgtggtttt cctttgaaaa acacgatgat aatatggcca ccatgtaccc atacgacgtc
     7441 ccagactacg ctatgaccga atacaagctt gttgttgttg gcgccgtcgg tgtgggcaag
     7501 agtgcgctga ccatccagct gatccagaac cattttgtgg acgaatacga ccccactata
     7561 gaggattcct accggaagca ggtggtcatt gatggggaga cgtgcctgtt ggacatcctg
     7621 gataccgccg gccaggagga gtacagcgcc atgcgggacc agtacatgcg caccggggag
     7681 ggcttcctgt gtgtgtttgc catcaacaac accaagtctt ttgaggacat ccaccagtac
     7741 agggagcaga tcaaacgggt gaaggactcg gatgacgtgc ccatggtgct ggtggggaac
     7801 aagtgtgacc tggctgcacg cactgtggaa tctcggcagg ctcaggacct cgcccgaagc
     7861 tacggcatcc cctacatcga gacctcggcc aagacccggc agggagtgga ggatgccttc
     7921 tacacgttgg tgcgtgagat ccggcagcac aagctgcgga agctgaaccc tcctgatgag
     7981 agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gacctgcagg caattcgtcg
     8041 agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc
     8101 ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     8161 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8221 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8281 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8341 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8401 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8461 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8521 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8581 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8641 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8701 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa
     8761 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     8821 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     8881 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8941 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     9001 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     9061 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     9121 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     9181 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     9241 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9421 gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9901 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    10081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11221 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    11281 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    11341 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    11401 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgctt
//
