LOCUS       sequence-197228-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mutant Becn1 leading to hyperactive 
            autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Rocchi A, Yamamoto S, Ting T, Fan Y, Sadleir K, Wang Y, Zhang W, 
            Huang S, Levine B, Vassar R, He C
  TITLE     A Becn1 mutation mediates hyperactive autophagic sequestration of 
            amyloid oligomers and improved cognition in Alzheimer's disease.
  JOURNAL   PLoS Genet. 2017 Aug 14;13(8):e1006962. doi: 
            10.1371/journal.pgen.1006962. eCollection 2017 Aug.
  PUBMED    28806762
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2017-08-14"; volume: "13(8):e1006962. doi"; pages: "
            10.1371/journal.pgen.1006962. eCollection 2017 Aug"
COMMENT     Sequence Label: lenti-HA-mBec1 F121A
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             290..316
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1734..1945
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1958..2226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2255..2264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2285..2947
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             3017..3070
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3071..3670
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3071..3090)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3567..3587
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3671..4259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3724..3744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4142..4153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4333..4566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4638..4759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4675..4694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4729..4748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4799..4934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4967..4983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4980..5002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4991..5007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5015..5021,5022..5039,5040..5045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5060..5081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5198..5215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5369..5957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6128..6919,6920..6988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6751..6770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6989..7093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7161..7179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7217..7239)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7339..7358
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7552..7574
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7566..7583
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7567..7583
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7598..7824
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7825..8005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8052..8177
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8670..8903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9088..9132
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9399..9515
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggttt atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagattc tagagtcgac gccaccatgt acccatacga
      301 tgttccagat tacgctgggg gaggcgaggg gtctaaggcg tccagcagca ccatgcaggt
      361 gagcttcgtg tgccagcgct gtagccagcc tctgaaactg gacacgagct tcaagatcct
      421 ggaccgggtc accatccagg aactcacagc tccattactt accacagccc aggcgaaacc
      481 aggagagacc caggaggaag aggctaatag cggtgaagaa cctttcattg aaactcgcca
      541 ggatggtgtc tctcgaagat tcatcccccc agccaggatg atgtctacag aaagtgctaa
      601 tagcttcact ctgatcgggg aggcatctga tggtggcacc atggagaacc tcagccggag
      661 actcaaggtc actggagacc tggctgacat catgtcgggc cagaccgatg tggatcaccc
      721 gctgtgtgag gaatgcacag acactctttt agaccagctg gacactcagc tcaatgtcac
      781 tgagaatgaa tgtcagaact acaaacgctg tttggagatc ctggagcaga tgaatgagga
      841 tgacagtgag cagctgcaga gggagctgaa ggagctggcc ttggaggagg agaggctgat
      901 ccaggagctg gaagatgtgg aaaagaaccg caaggtggtg gcagagaacc tggagaaggt
      961 ccaggctgag gcggagagat tggaccagga ggaagctcag taccagcggg agtatagtga
     1021 gtttaaaagg cagcagctgg agttggatga cgaactcaag agtgtggaga accaggtgcg
     1081 ctacgcccag atccagctgg acaagctcaa gaagaccaat gtcttcaatg ccaccttcca
     1141 tatctggcac agcggacagt ttggcacaat caataatttc agactgggtc gcttgcccag
     1201 cgttccagtg gagtggaatg aaatcaatgc tgcctggggc cagacagtgc tgctgctcca
     1261 tgctttggcc aataagatgg gtctgaagtt tcagaggtac cgacttgttc cctatggaaa
     1321 tcattcctat ctggagtctc tgacagacaa atctaaggag ttgccgttat actgttctgg
     1381 gggtttgcgg tttttctggg acaacaagtt tgaccatgca atggtagctt ttctggactg
     1441 tgtgcagcag ttcaaagaag aggtggaaaa aggagagact cgattttgtc ttccgtacag
     1501 gatggacgtg gagaaaggca agattgaaga cactggaggc agtggcggct cctattccat
     1561 caaaacccag tttaactcgg aggagcagtg gacaaaagcg ctcaagttca tgctgaccaa
     1621 tctcaagtgg ggtcttgcct gggtgtcctc acagttctat aacaagtgag aattcgaatt
     1681 taaatcggat ccgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     1741 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     1801 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     1861 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     1921 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     1981 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     2041 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     2101 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     2161 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     2221 cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg
     2281 cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga
     2341 gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag
     2401 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt
     2461 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa
     2521 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag
     2581 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg
     2641 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga
     2701 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct
     2761 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat
     2821 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct
     2881 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc
     2941 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg
     3001 ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     3061 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3121 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3181 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3241 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     3301 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     3361 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     3421 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     3481 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     3541 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     3601 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     3661 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3721 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3781 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3841 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3901 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3961 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4021 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4081 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4141 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4201 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4261 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4321 aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt
     4381 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4441 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4501 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4561 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4621 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4681 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4741 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4801 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4861 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4921 cttttttgga ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc
     4981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     7141 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     7201 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7261 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7321 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     7381 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     7441 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     7501 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     7561 gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt agtcttatgc aatactcttg
     7621 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7681 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7741 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     7801 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     7861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7981 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8041 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     8101 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     8161 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8221 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8281 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8341 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8401 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8461 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggcca
     8521 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8581 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8641 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8701 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8761 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8821 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8881 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8941 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     9001 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     9061 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     9121 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9181 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9241 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9301 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     9361 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     9421 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     9481 gaattacaaa aacaaattac aaaattcaaa attttatcga tactagtatt atgcccagta
     9541 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     9601 catg
//
