LOCUS       sequence-197231-       10826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expressing translational fusion between blue fluorescent 
            protein and Erk2 bearing the D319N mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Wilson MZ, Ravindran PT, Lim WA, Toettcher JE
  TITLE     Tracing Information Flow from Erk to Target Gene Induction Reveals 
            Mechanisms of Dynamic and Combinatorial Control.
  JOURNAL   Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 
            10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17.
  PUBMED    28826673
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.07.016"; journalName: "Mol Cell"; date: 
            "2017-09-7- 7"; volume: "67"; issue: "5"; pages: "757-769.e5"
COMMENT     Sequence Label: pHR SFFVp BFP-Erk D319N
FEATURES             Location/Qualifiers
     source          1..10826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    245..362
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        515..922
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             997..1695
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             2749..2784
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     misc_feature    2885..3473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2938..2958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3356..3367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3476..3492)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3477..3493)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3583..3816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3841..3859)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3842..3859)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4149..4168)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4382..4401)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4501..4523
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4561..4579)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4647..4751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4752..4820,4821..5612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4970..4989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5783..6371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6272..6291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6525..6542
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6612..6632)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6617..6946
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6797..6932
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8080..8145
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8275..8295
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8720..8854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8757..8776)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8811..8830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9022..9655
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9702..9827
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10324..10557
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10742..10786
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
       61 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
      121 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
      181 acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca gtattcatcc
      241 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      301 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      361 ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat
      421 tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
      481 agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa
      541 aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac
      601 gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca
      661 gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg
      721 cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg
      781 aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc
      841 gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct
      901 ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca cgcgtcaagt
      961 ggagcaaggc aggtggacag tggatccgga gctaccatga gcgagctgat taaggagaac
     1021 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     1081 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     1141 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     1201 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     1261 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     1321 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     1381 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     1441 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     1501 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     1561 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     1621 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     1681 gggcacaagc ttaatgctca agcttcgaat tctgcagtcg acggtaccgc gggcccggag
     1741 atggtccgcg ggcaggtgtt cgacgtaggg ccgcgctaca ccaacctctc gtacatcgga
     1801 gaaggcgcct acggcatggt ttgctctgct tatgataatc tcaacaaagt tcgagttgct
     1861 atcaagaaaa tcagtccttt tgagcaccag acctactgtc aaagaaccct aagagagata
     1921 aaaatcttac tgcgcttcag acatgagaac atcattggca tcaatgacat catccgggca
     1981 ccaaccattg agcaaatgaa agatgtatat atagtacagg acctcatgga gacggacctt
     2041 tacaagctct tgaagacaca gcacctcagc aatgaccaca tctgctattt tctttatcag
     2101 atcctgagag ggctaaagta tatccattca gctaacgttc tgcaccgtga cctcaagcct
     2161 tccaacctcc tgctgaacac cacttgtgat ctcaagatct gtgactttgg ccttgcccgt
     2221 gttgcagatc cagatcatga tcacacaggg ttcttgacag agtacgtagc cacacgttgg
     2281 tacagagctc cagaaattat gttgaattcc aagggttata ccaagtccat tgatatttgg
     2341 tctgtgggct gcatcctggc agagatgcta tccaacaggc ctatcttccc aggaaagcat
     2401 taccttgacc agctgaatca catcctgggt attcttggat ctccatcaca ggaagatctg
     2461 aattgtataa taaatttaaa agctagaaac tatttgcttt ctctcccgca caaaaataag
     2521 gtgccatgga acaggttgtt cccaaatgct gactccaaag ctctggattt actggataaa
     2581 atgttgacat ttaaccctca caagaggatt gaagttgaac aggctctggc ccacccatac
     2641 ctggagcagt attatgaccc aagtaatgag cccattgctg aagcgccatt caagtttgac
     2701 atggagttgg acgacttacc taaggagaag ctcaaagaac tcatttttga agagactgct
     2761 agattccagc caggatacag atcttaagcg gccgcaactc ccacctgcaa catgcgtgac
     2821 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     2881 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2941 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3001 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3061 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3121 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3181 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3241 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3301 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3361 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3421 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3481 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     3541 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3601 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3781 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     3841 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     3901 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     3961 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     4021 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     4081 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     4141 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     4201 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     4261 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     4321 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     4381 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4441 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4501 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4561 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4621 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4681 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4741 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4801 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4861 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4921 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4981 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5041 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5101 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5161 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5221 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5281 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5341 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5401 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5461 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5521 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5581 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5641 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5701 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5761 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5821 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5881 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5941 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6001 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6061 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6121 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6181 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6241 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6301 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6361 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6421 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6481 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6541 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6601 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6721 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6781 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6841 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6901 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     6961 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     7021 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     7081 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     7141 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     7201 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     7261 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     7321 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     7381 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     7441 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     7501 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     7561 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     7621 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     7681 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     7741 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     7801 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     7861 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     7921 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     7981 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     8041 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     8101 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     8161 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     8221 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     8281 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     8341 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     8401 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     8461 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     8521 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     8581 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     8641 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8701 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8761 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8821 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     8881 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     8941 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9001 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     9061 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     9121 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     9181 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     9241 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     9301 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     9361 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     9421 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     9481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9661 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9721 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9781 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9841 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9901 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9961 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10021 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10081 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10141 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
    10201 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10261 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10321 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10381 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10441 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10501 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10561 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10621 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10681 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10741 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10801 cataacaaat tggctgtggt atataa
//
