LOCUS       sequence-197233-       11039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TREAT-5'TOP reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger 
            J, Stadler MB, Giorgetti L, Chao JA
  TITLE     The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in
            Single Cells.
  JOURNAL   Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 
            10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
  PUBMED    29056324
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.09.030"; journalName: "Mol Cell"; date: 
            "2017-11-2- 2"; volume: "68"; issue: "3"; pages: "615-625.e9"
COMMENT     Sequence Label: pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7
            1...
FEATURES             Location/Qualifiers
     source          1..11039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3716..3763)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    join(3870..3888,3889..3911,3912..3930,3931..3953,3954..3972,
                     3973..3995,3996..4014,4015..4037,4038..4056,4057..4079,
                     4080..4098,4099..4121,4122..4140)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4173..4211
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4287..4419
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4402..4421
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4464..4482
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4486..4495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4492..5427
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8832..8966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8910..8929
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(9074..9107)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9160..9176)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9192..9214)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9625..9982
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10045..10638
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtcatcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcc
     2641 aagctaagtg aaagactctg tctcaaaaac caacctgaca cctgagccgg agcgttggct
     2701 ctcactacca tagaatattt tttcacaaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca cacagtcata cacatagaca cagtcacaca cacacacatg cacacacaca
     2821 cacacacact cacacacaca catacataca cacattcaca cacacccata cacacatttt
     2881 cacagtcaca cacacaaaca caatcacaca cacacacatc aatcaatcaa tcaatcaata
     2941 taccaaaaat gatagctcat acctgtaatc ttagatgtgg gaaggctaaa acagcaggct
     3001 acccatgact tcaaggtcac cctgggttac agagtagcta atagtctagc ttgaactata
     3061 gtgtaggatc tttaaaaaaa aattaaccaa ccaaccaaca aacaaacaaa caaacaacaa
     3121 caacaacaaa tggaagacag aggacaaaaa aaaataaaag aaaaagtaga aagccaatgg
     3181 agatactatc ttaaaattag aaatatttat gacacattct gaatactcca tgtttatttc
     3241 aaggaagcca atgtaacttt aagtactatg gccatttaat gtaaatactt aagaaaaaaa
     3301 accaaattaa ttttgataca tgctgcatgt gtgaaagacc cccgctgacg ggtagtcaat
     3361 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     3421 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     3481 tgaggggttg tgggctcgag ctcggggagc agaagcgcgc gaacagaagt cgagctagct
     3541 tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct
     3601 tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa
     3661 ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatccg gatctgaagt
     3721 tcctatactt tctagagaat aggaacttcg gaataggaac ttcgcggcag atcttttgcg
     3781 gccgctttgg gccggccgaa ttcgcgcaaa tttaaccctc actaaaggga acaaaagctg
     3841 ggtaccgggc cccccctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     3901 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     3961 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4021 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4081 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4141 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     4201 taagcagagc tcgtttaggc caccgtcctc tcttcctcgg cgctgcctac ggaggtggca
     4261 gccatctcct tctcggcacc ggtcaggtaa gtatcaaggt tacaagacag gtttaaggag
     4321 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     4381 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
     4441 acagctctta aggctagagt acttaatacg actcactata ggctagccac catggcttcc
     4501 aaggtgtacg accccgagca acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc
     4561 tgcaagcaaa tgaacgtgct ggactccttc atcaactact atgattccga gaagcacgcc
     4621 gagaacgccg tgatttttct gcatggtaac gctgcctcca gctacctgtg gaggcacgtc
     4681 gtgcctcaca tcgagcccgt ggctagatgc atcatccctg atctgatcgg aatgggtaag
     4741 tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc actacaagta cctcaccgct
     4801 tggttcgagc tgctgaacct tccaaagaaa atcatctttg tgggccacga ctggggggct
     4861 tgtctggcct ttcactactc ctacgagcac caagacaaga tcaaggccat cgtccatgct
     4921 gagagtgtcg tggacgtgat cgagtcctgg gacgagtggc ctgacatcga ggaggatatc
     4981 gccctgatca agagcgaaga gggcgagaaa atggtgcttg agaataactt cttcgtcgag
     5041 accatgctcc caagcaagat catgcggaaa ctggagcctg aggagttcgc tgcctacctg
     5101 gagccattca aggagaaggg cgaggttaga cggcctaccc tctcctggcc tcgcgagatc
     5161 cctctcgtta agggaggcaa gcccgacgtc gtccagattg tccgcaacta caacgcctac
     5221 cttcgggcca gcgacgatct gcctaagatg ttcatcgagt ccgaccctgg gttcttttcc
     5281 aacgctattg tcgagggagc taagaagttc cctaacaccg agttcgtgaa ggtgaagggc
     5341 ctccacttca gccaggagga cgctccagat gaaatgggta agtacatcaa gagcttcgtg
     5401 gagcgcgtgc tgaagaacga gcagtaagtc gacagatcct acctgcaaag caccagagga
     5461 tatggcctcg gtgcctcagc atcttcagat gtgctgtcac tgtccaatct tcaagccagc
     5521 agagcatatg ggctcgctgg ctccactgtc attacagtgg acctgatctt ctccaatcaa
     5581 aggagcagag gatatggcct cgctccctcc gactacatca ggctggaagt gacatccatc
     5641 attcatccca gcagagcata tgggctcgct gggaccacag catgcctcga agtgctgaga
     5701 gactatcatc aaaccgacag aggatatggc ctcgtcggct cctactccct cctggtggaa
     5761 tacttctcca ctcttcaacc cagcagagca tatgggctcg ctgggtccat cagagataca
     5821 agatctctcg acagatccta cctgcaaagc accagaggat atggcctcgg tgcctcagca
     5881 tcttcagatg tgctgtcact gtccaatctt caagccagca gagcatatgg gctcgctggc
     5941 tccactgtca ttacagtgga cctgatcttc tccaatcaaa ggagcagagg atatggcctc
     6001 gctccctccg actacatcag gctggaagtg acatccatca ttcatcccag cagagcatat
     6061 gggctcgctg ggaccacagc atgcctcgaa gtgctgagag actatcatca aaccgacaga
     6121 ggatatggcc tcgtcggctc ctactccctc ctggtggaat acttctccac tcttcaaccc
     6181 agcagagcat atgggctcgc tgggtccatc agagatacaa gatctctcga cagatcctac
     6241 ctgcaaagca ccagaggata tggcctcggt gcctcagcat cttcagatgt gctgtcactg
     6301 tccaatcttc aagccagcag agcatatggg ctcgctggct ccactgtcat tacagtggac
     6361 ctgatcttct ccaatcaaag gagcagagga tatggcctcg ctccctccga ctacatcagg
     6421 ctggaagtga catccatcat tcatcccagc agagcatatg ggctcgctgg gaccacagca
     6481 tgcctcgaag tgctgagaga ctatcatcaa accgacagag gatatggcct cgtcggctcc
     6541 tactccctcc tggtggaata cttctccact cttcaaccca gcagagcata tgggctcgct
     6601 gggtccatca gagatacaag atctctcgac agatcctacc tgcaaagcac cagaggatat
     6661 ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt ccaatcttca agccagcaga
     6721 gcatatgggc tcgctggctc cactgtcatt acagtggacc tgatcttctc caatcaaagg
     6781 agcagaggat atggcctcgc tccctccgac tacatcaggc tggaagtgac atccatcatt
     6841 catcccagca gagcatatgg gctcgctggg accacagcat gcctcgaagt gctgagagac
     6901 tatcatcaaa ccgacagagg atatggcctc gtcggctcct actccctcct ggtggaatac
     6961 ttctccactc ttcaacccag cagagcatat gggctcgctg ggtccatcag agatacaaga
     7021 tctctcgagc cgcggtgcgt aagtctgtta acagaatgct gcatacgctg agtacttcga
     7081 aatgtcatcc tctgtctgac actgaacgta atccagacgc gtaagtcagg ccggaaaatt
     7141 cccgccaccg gaagttgagt agacggtgct gcctgcgact caaccccagg aggactgggt
     7201 gaacaaagct gcgaagtgat ccatgtaagc cctcagaacc gtctcggaaa gaggacccca
     7261 catgttgtag cttcaaggcc caatgtcaga ccacgccatg gcgtgccact ctgcggagag
     7321 tgcagtctgc gacagtgccc caggaggact gggtgaggat cctacctaca aacggcacga
     7381 gcatcagccg tgccgcccag catcttcatc actgtccaat cttcaagcgt gcacgatcac
     7441 ggcacgctcc actgtcatta cagtcttatc caatcaaact ccagcagcat cagcgctgga
     7501 gctccgacta catcaggaca tccatcattc aacgctcgac gatcacgcga gcgtccacag
     7561 catgcctcag acactatcat caaacggctg cacgatcacg gcagccgctc ctactgcctc
     7621 tacgttatcg actcttctgc gacgagcatc agccgtcgca acatcggaca taccagatcc
     7681 tacctacaaa cggcacgagc atcagccgtg ccgcccagca tcttcatcac tgtccaatct
     7741 tcaagcgtgc acgatcacgg cacgctccac tgtcattaca gtcttatcca atcaaactcc
     7801 agcagcatca gcgctggagc tccgactaca tcaggacatc catcattcaa cgctcgacga
     7861 tcacgcgagc gtccacagca tgcctcagac actatcatca aacggctgca cgatcacggc
     7921 agccgctcct actgcctcta cgttatcgac tcttctgcga cgagcatcag ccgtcgcaac
     7981 atcggacata ccagatccta cctacaaacg gcacgagcat cagccgtgcc gcccagcatc
     8041 ttcatcactg tccaatcttc aagcgtgcac gatcacggca cgctccactg tcattacagt
     8101 cttatccaat caaactccag cagcatcagc gctggagctc cgactacatc aggacatcca
     8161 tcattcaacg ctcgacgatc acgcgagcgt ccacagcatg cctcagacac tatcatcaaa
     8221 cggctgcacg atcacggcag ccgctcctac tgcctctacg ttatcgactc ttctgcgacg
     8281 agcatcagcc gtcgcaacat cggacatacc agatcctacc tacaaacggc acgagcatca
     8341 gccgtgccgc ccagcatctt catcactgtc caatcttcaa gcgtgcacga tcacggcacg
     8401 ctccactgtc attacagtct tatccaatca aactccagca gcatcagcgc tggagctccg
     8461 actacatcag gacatccatc attcaacgct cgacgatcac gcgagcgtcc acagcatgcc
     8521 tcagacacta tcatcaaacg gctgcacgat cacggcagcc gctcctactg cctctacgtt
     8581 atcgactctt ctgcgacgag catcagccgt cgcaacatcg gacataccag atcttcaatc
     8641 gatttcgcgc gggatccaga ccacctcccc tgcgagctaa gctggacagc caatgacggg
     8701 taagagagtg acatttttca ctaacctaag acaggagggc cgtcagagct actgcctaat
     8761 ccaaagacgg gtaaaagtga taaaaatgta tcactccaac ctaagacagg cgcagcttcc
     8821 gagggatttg agatccagac atgataagat acattgatga gtttggacaa accaaaacta
     8881 gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa
     8941 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     9001 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     9061 ctgattatga tctgaagttc ctatactatt tgaagaatag gaacttcgga ataggaactt
     9121 cggatctcat atggccatat gggggcgcct agagaaggag tgagggctgg ataaagggag
     9181 gatcgaggcg gggtcgaacg aggaggttca agggggagag acggggcgga tggaggaaga
     9241 ggaggcggag gcttagggtg tacaaagggc ttgacccagg gaggggggtc aaaagccaag
     9301 gcttcccagg tcacgatgta ccccacctgg tctgggtgtc catgcgggcc aggtgaaaag
     9361 accttgatct taacctgggt gatgaggtct cggttaaagg tgccgtctcg cggccatccg
     9421 acgttaaagg ttggccattc tgcagagcag aaggtaaccc aacgtctctt cttgacatct
     9481 accgactggt tgtgagcgat ccgctcgaca tctttccagt gacctaaggt caaacttaag
     9541 ggagtggtaa caggctagcc cgggcccata ttttcagaca aatacagaaa cacagtcaga
     9601 cagagacaac acagaacgat gctgcagcag acaagacgcg cggcgcggct tcggtcccaa
     9661 accgaaagca aaaattcaga cggaggcggg aactgtttta ggttctcgtc tcctaccaga
     9721 accacatatc cctcctctaa ggggggtgca ccaaagagtc ctaaacgatc gggattttag
     9781 gactcaggtc gggccacaaa aacggccccc gaagtccctg ggacgtctcc cagggttgcg
     9841 gccgggtgtt ccgaactcgt cagttccacc acgggtccgc cagatacaga gctagttagc
     9901 taactagtac cgacgcaggc gcataaaatc agtcatagac actagacaat cggacagaca
     9961 cagataagtt gctggccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
    10021 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
    10081 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
    10141 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
    10201 ggttgtgggc tcttttattg agctcgggga gcataagcgc gcgaacagaa gcgagaagcg
    10261 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
    10321 aacatctgat ggttctctag aaactgctga gggctggacc gcatctgggg accatctgtt
    10381 cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg cccatattca
    10441 gctgttccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg
    10501 tttggcccat attcagctgt tccatctgtt cctgaccttg atctgaactt ttctattctc
    10561 agttatgtat ttttccatgc cttgcaaaat ggcgttactt aagctagctt gccaaaccta
    10621 caggtggggt ctttcattca catgtatatg tcaaaaataa aaatcaacta attgactagt
    10681 aattaatatg actggcataa tgggaaattg atcctgacag atgcaaactg gcttgctcag
    10741 cagcgcattt atgttgtcaa ctgaggaagg aaacgttaat gacagaaact ctaagtaatt
    10801 tccacgttta tctattttta tttatactag ctttggtaac aggaatattg cagcattcat
    10861 gcacattgaa acccttatga aataaaaaca tctgtgcatt taaaatggaa ttaacatttt
    10921 aaatgttaaa aaaagctggc ttagcttccc cccgccccct agggcataga acaagtcaaa
    10981 tgctttatat atttgagttt gggatgtatt aggaaactcc taagagcaaa gctagcttg
//
