LOCUS       sequence-19724-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Li B, de Lange T
  TITLE     Rap1 affects the length and heterogeneity of human telomeres.
  JOURNAL   Mol Biol Cell. 2003 Dec . 14(12):5060-8.
  PUBMED    14565979
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2003 Dec . 14(12):5060-8."
COMMENT     Sequence Label: pLPC FLAG hRap1 delta Br delta M
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc tgccgccacc atggactaca
      121 aagacgatga cgataaagga tctgcggagg cgttggaatt gggcaaagac cccaacgggc
      181 ccacccattc ctcgactctg ttcgtgaggg acgacggcag ctccatgtcc ttctacgtgc
      241 ggcccagccc ggccaagcgt ggatcctacc tcaagcacct gcggggccag gagcataagt
      301 acctgctggg ggacgcgccg gtgagcccct cctcccagaa gctcaagcgg aaggcggagg
      361 aggacccgga ggccgcggat agcggggaac cacagaataa gagaactcca gatttgcctg
      421 aagaagagta tgtgaaggaa gaaatccagg agaatgaaga agcagtcaaa aagatgcttg
      481 tggaagccac ccgggagttt gaggaggttg tggtggatga gagccctcct gattttgaaa
      541 tacatataac tatgtgtgat gatgatccac ccacacctga ggaagactca gaaacacagc
      601 ctgatgagga ggaagaagaa gaagaagaaa aagtttctca accagaggtg ggagctgcca
      661 ttaagatcat tcggcagtta atggagaagt ttaacttgga tctatcaaca gttacacagg
      721 ccttcctaaa aatagtggtg agctgg
//
