LOCUS       sequence-19725-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Li B, de Lange T
  TITLE     Rap1 affects the length and heterogeneity of human telomeres.
  JOURNAL   Mol Biol Cell. 2003 Dec . 14(12):5060-8.
  PUBMED    14565979
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2003 Dec . 14(12):5060-8."
COMMENT     Sequence Label: pLPC FLAG hRap1 delta BR delta CT
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatct gccgccacca tggactacaa
      121 agacgatgac gataaaggat ctgcggaggc gttggatttg ggcaaagacc ccaacgggcc
      181 cacccattcc tcgactctgt tcgtgaggga cgacggcagc tccatgtcct tctacgtgcg
      241 gcccagcccg gccaagcgtg gatccctgga gccctatcgg ctgggccccg cctcggcggc
      301 ggacaccggc tcggaagcaa agcccggggc cctggccgag ggcgccgcgg agccggagcc
      361 gcagcggcac gccgggcgga tcgccttcac ggatgcggac gacgtagcca tccttaccta
      421 cgtgaaggaa aatgcccgct cgcccagctc cgtcaccggt aacgccttgt ggaaagcgat
      481 ggagaagagc tcgctcacgc agcactcgtg gcagtccctg aaggaccgct acctcaagca
      541 cctgcggggc caggagcata agtacctgct gggggacgcg ccggtgagcc cctcctccca
      601 gaagctcaag cggaaggcgg aggaggaccc ggaggccgcg gatagcgggg aaacacagaa
      661 taagagaact ccagatttgc ct
//
