LOCUS       sequence-197264-       11166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPGAL80Uw-6
FEATURES             Location/Qualifiers
     source          1..11166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1969..2035
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    3363..3951
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3416..3436)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3834..3845)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          4043..4108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4238..4258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4530..4651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4567..4586)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4621..4640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(5688..6302,6373..6504,6708..7023,7085..7739,
                     7814..8087,8489..8560))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9012..9030
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9255..9324
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9411..9999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9491..9510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10170..10961,10962..11030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10793..10812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11031..11135)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accaaaaggt aggttcaacc
     1981 actgatgcct aggcacaccg aaacgactaa ccctaattct tatcctttac ttcaggctag
     2041 caatcaaaat ggattacaac aaaaggagta gtgtgagtac ggtgccgaat gctgctccca
     2101 ttcgcgtggg cttcgtggga ttgaacgcgg ctaagggttg ggccattaaa acgcattatc
     2161 cagccatact gcagctgagc tcccagttcc aaataacagc gctttattcc cccaagatcg
     2221 agacgtccat tgcgacaatt cagaggctga agttgtccaa cgccacagct ttcccaacac
     2281 tcgagagctt cgcctcgagc tccacgatcg atatgattgt gatcgcaatc caggtggctt
     2341 cccactacga ggtggtaatg cccctgctcg agttttcgaa gaacaatcct aatctgaagt
     2401 atttgtttgt ggaatgggca ctggcctgct cgctcgatca ggcggagtcg atctataagg
     2461 ccgctgcaga gcggggcgtg caaacaataa tcagtctgca gggacgaaag agcccgtaca
     2521 ttttgcgggc aaaagagctt atctcccagg gctacatcgg cgatataaat agcatcgaga
     2581 ttgcaggtaa cggaggttgg tacggttacg agcggccggt gaaaagcccg aaatacattt
     2641 acgagatcgg aaacggagtt gatctggtga ccaccacgtt cggtcacacg atagatatat
     2701 tgcagtacat gaccagttcg tacttcagcc gtatcaatgc catggtgttt aacaacattc
     2761 cagaacagga gctcattgat gagaggggca atcgcctggg ccagcgggtc ccaaagactg
     2821 tcccagatca tctcttgttc cagggcacgc tgctcaacgg aaatgttcct gtgtcctgtt
     2881 cctttaaggg aggcaagcct acgaaaaagt tcaccaagaa tctggtcata gatatccatg
     2941 gtacaaaggg tgatctgaag ctggagggtg acgctggatt tgcggaaatc tcgaatctcg
     3001 ttctgtatta ttcgggcacc cgcgccaatg attttcccct ggccaatggt cagcaggcac
     3061 ccttggaccc cggctacgac gcaggcaagg agattatgga ggtgtatcac ctccgaaact
     3121 acaacgcgat cgttggtaat atacaccggc tctaccagag tatcagcgat ttccacttca
     3181 acaccaagaa aatccccgag cttccatcgc agttcgtaat gcaaggcttc gatttcgaag
     3241 gctttcccac cctgatggat gccttgatct tgcatcgcct gattgaaagt gtctacaagt
     3301 cgaatatgat gggcagtacc ctgaatgttt ccaacatctc ccactactcg ctttaaaagc
     3361 ttaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgaagctt
     3961 cctagggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     4021 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     4081 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     4141 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     4201 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     4261 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     4321 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     4381 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     4441 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4501 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4561 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4621 gtggtttgtc caaactcatc aatgtatctt atcatgtcta ccggtactag ttctagagcg
     4681 gccgctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
     4741 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
     4801 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
     4861 cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg aggcgcgccc
     4921 tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat tatagttgtt
     4981 atttttgata tatataaaca acactattat gcccaccatt tttttgagat gcatctacac
     5041 aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca ctccgaacag
     5101 gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat agctgtgagg
     5161 gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt attactaatt
     5221 tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg cgcggaacta
     5281 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
     5341 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
     5401 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
     5461 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
     5521 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
     5581 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
     5641 ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt
     5701 cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc
     5761 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     5821 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     5881 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     5941 aaccatttga ggtatactgg caccgagccc gagttcaaga agaagccgcc aaagagcagg
     6001 aatggtatga taaccggcgg acccacagac agcgccatcg aggtcgagga gctggcgcag
     6061 gatattagat atccgaagga cgttgacaca ttggccacca gagtgaccag cgccaggcag
     6121 ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag
     6181 accagtggca ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg
     6241 tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac
     6301 acctaaatcg aatcgattca ttagaaagtt agtaaattat tgaaatgcaa atgtattcta
     6361 aacatgactt acatttatcg tggcaaagac gttttgaaag gtcatgttgg tcaggaagag
     6421 gaagatggct ccgttgatat tcatcacacc cacttgcgtg agttgttggc ccaaaaagat
     6481 gaggccaatc aagatggcaa ccatctgcaa attaaaatgt tactcgcatc tcattaatat
     6541 tcgcgagtta aatgaaattt atttatcttc tgcaaaacta taaactatac atctcattga
     6601 aaaaaactaa gaagggtgtg gaatcaggca attctatcta aaatctagcg aatttgtttc
     6661 caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt tgtctgaata
     6721 agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg ccacaggacc
     6781 gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg ctgctccagt
     6841 ggcttctcca gatttttggt ggccaacaac tgctccatat cccgggctac tttgctaatg
     6901 gcaaaattgt cgcatatctt ggcgatccga tcacgggact cgatctcccg tccgggcaca
     6961 acggccaaca cctgtacgta aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc
     7021 acgctggata ggagttgaga tgttatgtaa tactagatac ccttaataaa cacatcgaac
     7081 tcactaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta ccctgccctc
     7141 ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct gatgaatggt
     7201 caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga cgctgtgggc
     7261 ggtaaaggag tccagtccgg aggtgggctc atcgcagatc agaagcggcg gatcggttag
     7321 agcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt tcaccctgcc
     7381 gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga tcacctgatc
     7441 cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca tggcttggaa
     7501 aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct ggacataggc
     7561 gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc gcatcccgga
     7621 tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt
     7681 tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca cgccgcaaac
     7741 tgagtttcaa attggtaatt ggacccttta ttaagatttc acacagatca gccgactgcg
     7801 aatagaaact caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg
     7861 cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg ctgattgacc
     7921 gccccaaaga tgtccatatt gtgccaggca taggtgaggt tctcggctag ttggccgctc
     7981 cctgaaccgg agtcctccgg cggactgggt ggccggagcg tgccgtagtt tttggcctgc
     8041 ccgaagccct ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg caatgatagg
     8101 ggatctcaaa tatcaactac aagcgttatg ctcatctaac cccgaacaaa aagtaccccg
     8161 aagtatccta cgaagtaggt ttatactttt atttattttt tgtgcatcta ggatcagctt
     8221 aaaatatctg gttgttatat tttttgtaaa aaagaatata gtcgaaaatg aatgccttta
     8281 gatgtcttga tcatgatatg atctcaaaaa ttgtcttata tagcgagaac agctaccaga
     8341 ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg
     8401 cagttctcac gccgcagaca atttgatgtt gcaatcgcag ttcctataga tcaagtgaac
     8461 ttaagatgta tgcacatgta ctactcacat tgttcagatg ctcggcagat gggtgtttgc
     8521 tgcctccgcg aattaatagc tcctgatcct cttggcccat tgccgggatt tttcacactt
     8581 tcccctgctt acccacccaa aaccaatcac caccccaatc actcaaaaaa caaacaaaaa
     8641 taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg taaaccgctt
     8701 ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg acgctacgta
     8761 acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac ggcgatattt
     8821 ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat tttgagtgag
     8881 aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat ttaaaactga
     8941 tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt
     9001 tccgcaaaaa tgggttttat taacttacat acatactagg cgcgcccata tgttcggctt
     9061 gtcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc
     9121 gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc
     9181 gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact accgccacct cgacccgttc
     9241 atcatgatgg accagatggg tgaggtggag tacgcgcccg gggagcccaa gggcacgccc
     9301 tggcacccgc accgcggctt cgagaccgtg acctacatcg tcgacggtaa catgtgagca
     9361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9841 tacactagaa gaacagtatt tggtatctga gctctgctga agccagttac cttcggaaaa
     9901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11161 gacgtc
//
