LOCUS       sequence-19727-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Deplancke B, Mukhopadhyay A, Ao W, Elewa AM, Grove CA, Martinez NJ, 
            Sequerra R, Doucette-Stamm L, Reece-Hoyes JS, Hope IA, Tissenbaum 
            HA, Mango SE, Walhout AJ
  TITLE     A gene-centered C. elegans protein-DNA interaction network.
  JOURNAL   Cell. 2006 Jun 16. 125(6):1193-205.
  PUBMED    16777607
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jun 16. 125(6):1193-205."
COMMENT     Sequence Label: pMW#2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(455..578)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(648..666)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(649..666)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(674..690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(683..705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(919..938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
       61 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgatacagt agaaattaca
      121 gaaactttat cacgtttagt aagtatagag gctgaaaatc cagatgaagc cgaacgactt
      181 gtaagagaaa agtataagag ttgtgaaatt gttcttgatg cagatgattt tcaggactat
      241 gacactagcg tatatgaata ggtagatgtt tttattttgt cacacaaaaa agaggctcgc
      301 acctcttttt cttatttctt tttatgattt aatacggcat tgaggacaat agcgagtagg
      361 ctggatacga cgattccgtt tgagaagaac atttggaagg ctgtcggtcg agctcgaatt
      421 ctacaggtca ctaataccat ctaagtagtt gattcatagt gactgcatat gttgtgtttt
      481 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      541 attttacgtt tctcgttcaa cttttctata caaagttgat atcactagtc ggcggccgct
      601 ctagaggatc caagcttacg tacgcgtgca tgcgacgtca tagctcttct atagtgtcac
      661 ctaaattcaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      721 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      781 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
      841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcg
//
