LOCUS       sequence-197288-        2548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ac transposase with SP6 promoter for in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Chong-Morrison V, Mayes S, Simoes FC, Senanayake U, Carroll DS, 
            Riley PR, Wilson SW, Sauka-Spengler T
  TITLE     Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in 
            zebrafish.
  JOURNAL   Biol Open. 2023 Jun 15;12(6):bio059995. doi: 10.1242/bio.059995. 
            Epub 2023 Jun 27.
  PUBMED    37367831
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2023-06-15"; volume: "12(6):bio059995. doi"; pages: "
            10.1242/bio.059995. Epub 2023 Jun 27"
COMMENT     Sequence Label: pAC-SP6
FEATURES             Location/Qualifiers
     source          1..2548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     5'UTR           82..124
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      132..141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
       61 acactataga atacaagctt gcttgttctt tttgcagaag ctcagaataa acgctcaact
      121 ttggcagatc cgccaccatg ggtcctccag agaagaagcg taaggtagaa atggctattg
      181 ttcatgaacc acaaccacaa ccacaaccac aaccagaacc acaaccacag ccacaacctg
      241 aacccgaaga agaagcacca cagaagaggg caaagaagtg cacatcggat gtatggcagc
      301 atttcaccaa gaaggaaatt gaagtggagg tcgatggaaa gaaatacgtt caggtatggg
      361 ggcattgcaa ctttcctaat tgcaaggcta agtatagggc tgagggtcat catggaacaa
      421 gcggatttcg aaatcacttg agaacatcac atagtttagt taaaggtcag ttgtgtctaa
      481 aaagtgaaaa ggatcatggc aaagacataa atctcattga gccttataag tacgatgaag
      541 tggttagcct aaagaagctt catttggcaa taatcatgca tgaataccct ttcaatattg
      601 tagaacatga gtactttgtt gagtttgtta agtctctgcg ccctcacttt ccaataaagt
      661 cccgtgtcac tgctagaaaa tatatcatgg atttgtattt ggaagaaaaa gaaaagttgt
      721 atggaaaact aaaagatgtt cagtctcgct tcagtacaac tatggatatg tggacatctt
      781 gtcaaaataa gtcatacatg tgtgtcacca tccattggat tgatgatgat tggtgtctcc
      841 aaaaaagaat tgttggcttt tttcatgttg aagggcgcca cactggccaa aggttatcac
      901 aaaccttcac tgcaatcatg gttaagtgga acattgagaa aaaattgttt gccttgtctt
      961 tggataatgc tagtgcaaat gaagtagctg tgcacgatat aattgaggat ttgcaggaca
     1021 ctgattcaaa tctagtttgt gatggtgctt tctttcatgt gaggtgtgct tgtcacatac
     1081 tgaacttggt tgcaaaggat ggcttggctg taattgcagg aacaattgag aaaatcaaag
     1141 cgattgttct tgctgtaaaa tcttctcctt tgcagtggga agaactaatg aagtgtgcta
     1201 gtgaatgtga cttggataaa tctaaaggga tctcatatga tgtctcaact agatggaatt
     1261 caacctattt gatgttgagg gatgccttat attataagcc tgcactaata aggcttaaaa
     1321 caagtgatcc tcgcaggtac gatgcaattt gtcctaaagc cgaggagtgg aagatggcat
     1381 taactctttt taagtgtttg aagaagtttt ttgatctcac tgaactccta tctggtactc
     1441 aatattccac tgcaaattta ttttacaaag gtttctgtga gataaaggat ttgattgacc
     1501 aatggtgtgt tcatgaaaaa tttgtcatta ggagaatggc cgttgcaatg agtgaaaagt
     1561 ttgagaaata ttggaaagtg tctaatattg cactagctgt agcatgcttc cttgacccta
     1621 ggtacaagaa aatattgatt gagttctata tgaaaaaatt tcatggtgat tcatacaaag
     1681 ttcatgtaga tgactttgtt agggtcatta gaaaattgta tcaattctat tctagttgta
     1741 gtccttcagc tccaaagaca aagacaacta ctaatgatag tatggatgat accttgatgg
     1801 aaaatgaaga tgatgaattt caaaactatt tgcatgagtt gaaggattat gatcaagtag
     1861 agtcaaatga attggataaa tatatgtctg aacccctttt gaagcatagt ggtcagtttg
     1921 atattttatc atggtggagg ggaagggttg cagaatatcc tattctcacc caaattgcaa
     1981 gggatgtgct agcaatacaa gtgtcaactg ttgcttctga gtctgcgttc agtgctggtg
     2041 gtcgtgttgt tgatccttac cgcaatcgtc ttggttcgga gattgttgaa gctttgatat
     2101 gcacaaaaga ttgggtagca gcatctagaa aaggtgctac atattttcca acaatgattg
     2161 gtgatctcga ggtgctagac tctgttattg ctgctgcaac aaatcatgag aatcatatgg
     2221 atgaggatga agacgcaata gaattttcta agaataatga agatgtagca agtggctcct
     2281 ctccatgagc aatgtgtctt atgtttgttg acagatgagc cttggttgta atagtttatg
     2341 catgctaagt gctccagatg tgagcaagtg attatgaata tgtgtttaaa ctttatattg
     2401 tgtcatgtgt gctagtagac ttatatggct tttatgttag ccaagggggc cccgggattg
     2461 gttaccacta aaccagcctc aagaacaccc gaatggagtc tctaagctac ataataccaa
     2521 cttacacttt acaaaatgtt gtccccca
//
