LOCUS       sequence-197294-       14477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiral expression vector bearing the MS2 Coat Protein (MCP) fused 
            to mCherry as well as the nuclear marker histone 2b fused to two 
            copies of irFP. Also bears the hygromycin selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Wilson MZ, Ravindran PT, Lim WA, Toettcher JE
  TITLE     Tracing Information Flow from Erk to Target Gene Induction Reveals 
            Mechanisms of Dynamic and Combinatorial Control.
  JOURNAL   Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 
            10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17.
  PUBMED    28826673
REFERENCE   2  (bases 1 to 14477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.07.016"; journalName: "Mol Cell"; date: 
            "2017-09-7- 7"; volume: "67"; issue: "5"; pages: "757-769.e5"
COMMENT     Sequence Label: pHR SFFVp MCP-mCherry IRES h2b-diRFP
FEATURES             Location/Qualifiers
     source          1..14477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..465
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             650..694
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    979..1096
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1250..1657
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     2485..2504
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(2818..3391,3392..3394,3395..3403)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2985..3002)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3212..3231
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3422..3799
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3854..4801
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             4853..5803
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     protein_bind    complement(5827..5851)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5882..6470
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5935..5955)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6353..6364)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6526..7025
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6561..6584)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6958..6977
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(7861..7877)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7862..7878)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7968..8201
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8226..8244)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8227..8244)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8534..8553)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8767..8786)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8886..8908
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8946..8964)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9032..9136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9137..9205,9206..9997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9355..9374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10168..10756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10657..10676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10910..10927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10997..11017)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11002..11331
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11182..11317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12465..12530
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12660..12680
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13105..13239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13142..13161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13196..13215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             13407..14040
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    14087..14212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
       61 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
      121 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
      181 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
      241 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
      301 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
      361 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
      421 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
      481 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
      541 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
      601 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
      661 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
      721 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
      781 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
      841 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
      901 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
      961 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     1021 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     1081 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
     1141 gcttgatatc gaattccctg cagccccgat aaaataaaag attttattta gtctccagaa
     1201 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt
     1261 ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac
     1321 atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc
     1381 ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat
     1441 ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc
     1501 tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct
     1561 cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact
     1621 cggcgcgcca gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat
     1681 tctcacgcgt gtgatcggag ctaccatggc ttctaacttt actcagttcg ttctcgtcga
     1741 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga
     1801 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag
     1861 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc
     1921 gtacttaaat atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat
     1981 tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc
     2041 aaactccggc atctacggcg gatccggggg aatggtgagc aagggcgagg aggataacat
     2101 ggctatcatt aaagagttca tgcgcttcaa agttcacatg gagggttctg ttaacggtca
     2161 cgagttcgag atcgaaggcg aaggcgaggg ccgtccgtat gaaggcaccc agaccgccaa
     2221 actgaaagtg actaaaggcg gcccgctgcc ttttgcgtgg gacatcctgt cccctcagtt
     2281 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     2341 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     2401 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     2461 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     2521 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     2581 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     2641 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     2701 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     2761 catggacgag ctgtacaagt aataagcggc cgcaactccc accgtttaaa caattcgccc
     2821 ctctcccccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     2881 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     2941 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3001 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3061 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3121 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3181 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3241 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3301 atgctttacg tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     3361 gtggttttcc tttgaaaaac acgatgataa tatggccaca accgttaatt aaggagctac
     3421 catgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac
     3481 taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat
     3541 ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat
     3601 gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg
     3661 cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg
     3721 cctgctgctg cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac
     3781 caagtacacc agcgctaaga acatgagcgg cggcaccagt accagcagct caggctccgg
     3841 cggacatact gggatggctg aaggatccgt cgccaggcag cctgacctct tgacctgcga
     3901 cgatgagccg atccatatcc ccggtgccat ccaaccgcat ggactgctgc tcgccctcgc
     3961 cgccgacatg acgatcgttg ccggcagcga caaccttccc gaactcaccg gactggcgat
     4021 cggcgccctg atcggccgct ctgcggccga tgtcttcgac tcggagacgc acaaccgtct
     4081 gacgatcgcc ttggccgagc ccggggcggc cgtcggagca ccgatcactg tcggcttcac
     4141 gatgcgaaag gacgcaggct tcatcggctc ctggcatcgc catgatcagc tcatcttcct
     4201 cgagctcgag cctccccagc gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa
     4261 cagcgccatc cgccgcctgc aggccgccga aaccttggaa agcgcctgcg ccgccgcggc
     4321 gcaagaggtg cggaagatta ccggcttcga tcgggtgatg atctatcgct tcgcctccga
     4381 cttcagcggc gaagtgatcg cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct
     4441 gcactatcct gcctcaaccg tgccggcgca ggcccgtcgg ctctatacca tcaacccggt
     4501 acggatcatt cccgatatca attatcggcc ggtgccggtc accccagacc tcaatccggt
     4561 caccgggcgg ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc ccgtccatct
     4621 ggaattcatg cgcaacatag gcatgcacgg cacgatgtcg atctcgattt tgcgcggcga
     4681 gcgactgtgg ggattgatcg tttgccatca ccgaacgccg tactacgtcg atctcgatgg
     4741 ccgccaagcc tgcgagctag tcgcccaggt tctggcctgg cagatcggcg tgatggaaga
     4801 gtcagggcat ggcaccggca gcaccggcag cggcagcaca tccggaacta gtatggctga
     4861 aggatccgtc gccaggcagc ctgacctctt gacctgcgac gatgagccga tccatatccc
     4921 cggtgccatc caaccgcatg gactgctgct cgccctcgcc gccgacatga cgatcgttgc
     4981 cggcagcgac aaccttcccg aactcaccgg actggcgatc ggcgccctga tcggccgctc
     5041 tgcggccgat gtcttcgact cggagacgca caaccgtctg acgatcgcct tggccgagcc
     5101 cggggcggcc gtcggagcac cgatcactgt cggcttcacg atgcgaaagg acgcaggctt
     5161 catcggctcc tggcatcgcc atgatcagct catcttcctc gagctcgagc ctccccagcg
     5221 ggacgtcgcc gagccgcagg cgttcttccg ccgcaccaac agcgccatcc gccgcctgca
     5281 ggccgccgaa accttggaaa gcgcctgcgc cgccgcggcg caagaggtgc ggaagattac
     5341 cggcttcgat cgggtgatga tctatcgctt cgcctccgac ttcagcggcg aagtgatcgc
     5401 agaggatcgg tgcgccgagg tcgagtcaaa actaggcctg cactatcctg cctcaaccgt
     5461 gccggcgcag gcccgtcggc tctataccat caacccggta cggatcattc ccgatatcaa
     5521 ttatcggccg gtgccggtca ccccagacct caatccggtc accgggcggc cgattgatct
     5581 tagcttcgcc atcctgcgca gcgtctcgcc cgtccatctg gaattcatgc gcaacatagg
     5641 catgcacggc acgatgtcga tctcgatttt gcgcggcgag cgactgtggg gattgatcgt
     5701 ttgccatcac cgaacgccgt actacgtcga tctcgatggc cgccaagcct gcgagctagt
     5761 cgcccaggtt ctggcctggc agatcggcgt gatggaagag tgagcggccg cactcgagat
     5821 atctagaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
     5881 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5941 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6001 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6061 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6121 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6181 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6241 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6301 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6361 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6421 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct
     6481 gcagatatcc ggactagtga tctctcgagg ttaacgaatt ctaccgggta ggggaggcgc
     6541 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     6601 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     6661 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     6721 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     6781 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     6841 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     6901 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     6961 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     7021 tttcgacctg catcccgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     7081 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     7141 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     7201 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     7261 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     7321 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     7381 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     7441 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     7501 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     7561 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     7621 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     7681 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     7741 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     7801 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgataa
     7861 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     7921 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7981 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8041 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8101 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8161 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     8221 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     8281 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     8341 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     8401 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     8461 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     8521 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     8581 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     8641 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     8701 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     8761 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8821 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8881 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     8941 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     9001 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9061 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    10921 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    10981 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    11041 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    11101 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    11161 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    11221 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    11281 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
    11341 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
    11401 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    11461 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    11521 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    11581 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    11641 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
    11701 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
    11761 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
    11821 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
    11881 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
    11941 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
    12001 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
    12061 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
    12121 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
    12181 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
    12241 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
    12301 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
    12361 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
    12421 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
    12481 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
    12541 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
    12601 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
    12661 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
    12721 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
    12781 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
    12841 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
    12901 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
    12961 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
    13021 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    13081 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    13141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    13201 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    13261 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    13321 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    13381 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
    13441 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
    13501 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
    13561 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
    13621 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
    13681 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
    13741 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
    13801 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
    13861 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    13921 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    13981 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    14041 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    14101 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    14161 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    14221 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    14281 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    14341 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    14401 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    14461 gcatcaaagg atagaga
//
