LOCUS       sequence-197339-        9585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and
            Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Duan L, Hope J, Ong Q, Lou HY, Kim N, McCarthy C, Acero V, Lin MZ, 
            Cui B
  TITLE     Understanding CRY2 interactions for optical control of intracellular
            signaling.
  JOURNAL   Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8.
  PUBMED    28916751
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-15"; volume: "8(1):547. doi"; pages: " 
            10.1038/s41467-017-00648-8"
COMMENT     Sequence Label: CRY2high-mCherry-Raf1
FEATURES             Location/Qualifiers
     source          1..9585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..2416
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRRAQIMIGAA"
     CDS             2459..3166
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2604..2622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2872..2891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5179..5196)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5185..5409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5455..5883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5542..5561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5752..5773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5892..5912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5897..6226
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6077..6212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6139..6158
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6293..7087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6347..6366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6957..6976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7261..7382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7298..7317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7352..7371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7431..7447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7431..7447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7444..7466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7455..7471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7479..7485,7486..7503,7504..7509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7524..7545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7662..7679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7833..8418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7913..7932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8589..9380,9381..9449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9212..9231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9450..9554)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatgaagat ggacaaaaag actatagttt ggtttagaag
      961 agacctaagg attgaggata atcctgcatt agcagcagct gctcacgaag gatctgtttt
     1021 tcctgtcttc atttggtgtc ctgaagaaga aggacagttt tatcctggaa gagcttcaag
     1081 atggtggatg aaacaatcac ttgctcactt atctcaatcc ttgaaggctc ttggatctga
     1141 cctcacttta atcaaaaccc acaacacgat ttcagcgatc ttggattgta tccgcgttac
     1201 cggtgctaca aaagtcgtct ttaaccacct ctatgatcct gtttcgttag ttcgggacca
     1261 taccgtaaag gagaagctgg tggaacgtgg gatctctgtg caaagctaca atggagatct
     1321 attgtatgaa ccgtgggaga tatactgcga aaagggcaaa ccttttacga gtttcaattc
     1381 ttactggaag aaatgcttag atatgtcgat tgaatccgtt atgcttcctc ctccttggcg
     1441 gttgatgcca ataactgcag cggctgaagc gatttgggcg tgttcgattg aagaactagg
     1501 gctggagaat gaggccgaga aaccgagcaa tgcgttgtta actagagctt ggtctccagg
     1561 atggagcaat gctgataagt tactaaatga gttcatcgag aagcagttga tagattatgc
     1621 aaagaacagc aagaaagttg ttgggaattc tacttcacta ctttctccgt atctccattt
     1681 cggggaaata agcgtcagac acgttttcca gtgtgcccgg atgaaacaaa ttatatgggc
     1741 aagagataag aacagtgaag gagaagaaag tgcagatctt tttcttaggg gaatcggttt
     1801 aagagagtat tctcggtata tatgtttcaa cttcccgttt actcacgagc aatcgttgtt
     1861 gagtcatctt cggtttttcc cttgggatgc tgatgttgat aagttcaagg cctggagaca
     1921 aggcaggacc ggttatccgt tggtggatgc cggaatgaga gagctttggg ctaccggatg
     1981 gatgcataac agaataagag tgattgtttc aagctttgct gtgaagtttc ttctccttcc
     2041 atggaaatgg ggaatgaagt atttctggga tacacttttg gatgctgatt tggaatgtga
     2101 catccttggc tggcagtata tctctgggag tatccccgat ggccacgagc ttgatcgctt
     2161 ggacaatccc gcgttacaag gcgccaaata tgacccagaa ggtgagtaca taaggcaatg
     2221 gcttcccgag cttgcgagat tgccaactga atggatccat catccatggg acgctccttt
     2281 aaccgtactc aaagcttctg gtgtggaact cggaacaaac tatgcgaaac ccattgtaga
     2341 catcgacaca gctcgtgagc tactagctaa agctatttca agaacccgta gagcacagat
     2401 catgatcgga gcagcagaat tcgatagtgc tggtagtgct ggtagtgctg gtactagtat
     2461 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     2521 gcatatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2581 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     2641 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     2701 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2761 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     2821 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     2881 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     2941 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     3001 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3061 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3121 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc
     3181 tcgagtgatg gagcacatac agggagcttg gaagacgatc agcaatggtt ttggattcaa
     3241 agatgccgtg tttgatggct ccagctgcat ctctcctaca atagttcagc agtttggcta
     3301 tcagcgccgg gcatcagatg atggcaaact cacagatcct tctaagacaa gcaacactat
     3361 ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat gtgcgaaatg gaatgagctt
     3421 gcatgactgc cttatgaaag cactcaaggt gaggggcctg caaccagagt gctgtgcagt
     3481 gttcagactt ctccacgaac acaaaggtaa aaaagcacgc ttagattgga atactgatgc
     3541 tgcgtctttg attggagaag aacttcaagt agatttcctg gatcatgttc ccctcacaac
     3601 acacaacttt gctcggaaga cgttcctgaa gcttgccttc tgtgacatct gtcagaaatt
     3661 cctgctcaat ggatttcgat gtcagacttg tggctacaaa tttcatgagc actgtagcac
     3721 caaagtacct actatgtgtg tggactggag taacatcaga caactcttat tgtttccaaa
     3781 ttccactatt ggtgatagtg gagtcccagc actaccttct ttgactatgc gtcgtatgcg
     3841 agagtctgtt tccaggatgc ctgttagttc tcagcacaga tattctacac ctcacgcctt
     3901 cacctttaac acctccagtc cctcatctga aggttccctc tcccagaggc agaggtcgac
     3961 atccacacct aatgtccaca tggtcagcac caccctgcct gtggacagca ggatgattga
     4021 ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca gccctgtcca gtagccccaa
     4081 caatctgagc ccaacaggct ggtcacagcc gaaaaccccc gtgccagcac aaagagagcg
     4141 ggcaccagta tctgggaccc aggagaaaaa caaaattagg cctcgtggac agagagattc
     4201 aagctattat tgggaaatag aagccagtga agtgatgctg tccactcgga ttgggtcagg
     4261 ctcttttgga actgtttata agggtaaatg gcacggagat gttgcagtaa agatcctaaa
     4321 ggttgtcgac ccaaccccag agcaattcca ggccttcagg aatgaggtgg ctgttctgcg
     4381 caaaacacgg catgtgaaca ttctgctttt catggggtac atgacaaagg acaacctggc
     4441 aattgtgacc cagtggtgcg agggcagcag cctctacaaa cacctgcatg tccaggagac
     4501 caagtttcag atgttccagc taattgacat tgcccggcag acggctcagg gaatggacta
     4561 tttgcatgca aagaacatca tccatagaga catgaaatcc aacaatatat ttctccatga
     4621 aggcttaaca gtgaaaattg gagattttgg tttggcaaca gtaaagtcac gctggagtgg
     4681 ttctcagcag gttgaacaac ctactggctc tgtcctctgg atggccccag aggtgatccg
     4741 aatgcaggat aacaacccat tcagtttcca gtcggatgtc tactcctatg gcatcgtatt
     4801 gtatgaactg atgacggggg agcttcctta ttctcacatc aacaaccgag atcagatcat
     4861 cttcatggtg ggccgaggat atgcctcccc agatcttagt aagctatata agaactgccc
     4921 caaagcaatg aagaggctgg tagctgactg tgtgaagaaa gtaaaggaag agaggcctct
     4981 ttttccccag atcctgtctt ccattgagct gctccaacac tctctaccga agatcaaccg
     5041 gagcgcttcc gagccatcct tgcatcgggc agcccacact gaggatatca atgcttgcac
     5101 gctgaccacg tccccgaggc tgcctgtctt ctaagcggcc gctcgagtct agagggcccg
     5161 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5221 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5281 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5341 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5401 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5461 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5521 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5581 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5641 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5701 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5761 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5821 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5881 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5941 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6001 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6061 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6121 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6181 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6241 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6301 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6361 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6421 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6481 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6541 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6601 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6661 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6721 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6781 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6841 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6901 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6961 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7021 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7081 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     7141 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7201 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7261 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7321 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7381 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7441 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7501 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7561 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7621 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7681 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8341 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     8401 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8461 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8521 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8581 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8641 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8701 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8761 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8821 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8881 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8941 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9001 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9061 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9121 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9181 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9241 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9301 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9361 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9421 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9481 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9541 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
