LOCUS       sequence-197473-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  codes for a fusion of fluorescent protein mGarnet2 and lifeact that 
            targets F-actin in live cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Matela G, Gao P, Guigas G, Eckert AF, Nienhaus K, Nienhaus GU
  TITLE     A far-red emitting fluorescent marker protein, mGarnet2, for 
            microscopy and STED nanoscopy.
  JOURNAL   Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 
            10.1039/c6cc09081h.
  PUBMED    28044150
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Commun (Camb)"; date: "2017-01-10- 10"; volume: "53"; issue: 
            "5"; pages: "979-982"
COMMENT     Sequence Label: LifeAct-mGarnet2
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..681
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSKTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQLRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYNHMALK
                     VDGGGHLHCSLVTTYRSKKTVGNIKMPGIHAVDRRLERLEESDNEMFVVQREHAVAKFA
                     GLGGG"
ORIGIN
        1 atgaacagcc tgatcaaaga aaacatgcgg atgaaggtgg tgctggaagg cagcgtgaac
       61 ggccaccagt tcaagtgcac cggcgagggc gagggcaacc cctacatggg cacccagacc
      121 atgcggatca aagtgatcga gggcggacct ctgcccttcg ccttcgacat cctggccaca
      181 tccttcatgt acggcagcaa gaccttcatc aagtacccca agggcatccc cgatttcttc
      241 aagcagagct tccccgaggg cttcacctgg gagagagtga ccagatacga ggacggcggc
      301 gtgatcaccg tgatgcagga caccagcctg gaagatggct gcctggtgta ccatgcccag
      361 ctcaggggcg tgaattttcc cagcaacggc gccgtgatgc agaagaaaac caagggctgg
      421 gagcccaaca ccgagatgat gtaccccgct gacggcggac tgagaggcta caaccacatg
      481 gccctgaagg tggacggcgg agggcacctg cactgcagcc tggtgaccac ctaccggtcc
      541 aagaaaaccg tgggcaacat caagatgccc ggcatccacg ccgtggaccg gcggctggaa
      601 aggctggaag agtccgacaa cgagatgttc gtggtgcagc gggagcacgc cgtggccaag
      661 ttcgccggcc tgggcggagg gtga
//
