LOCUS       sequence-197514-       12066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP
            and dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe3-WT
FEATURES             Location/Qualifiers
     source          1..12066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        75..378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..659
                     /label=chicken beta-actin promoter
     intron          660..1668
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1727..2428
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    2572..2627
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   4040..4074
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        4420..4723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4726..5004
                     /label=chicken beta-actin promoter
     intron          5005..6013
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     regulatory      6066..6075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6072..6773
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6205..6223)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6473..6492
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6667..6690
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6774..6827
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6828..7424
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7321..7341
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7568..7623
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   8153..8215
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9214..9234)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9214..9232)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9253..9269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9253..9269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9266..9288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9277..9293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9301..9307,9308..9325,9326..9331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9346..9367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      9604..10192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10093..10112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10346..10363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(10414..11205,11206..11274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11037..11056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11275..11379)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11405..11860)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11542..11563)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11754..11773
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11987..12009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12001..12018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12002..12018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12028..12047
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12028..12046
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
       61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      361 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
      421 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
      481 gggggcgggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc
      721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      781 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      841 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg
      961 ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg
     1021 tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1081 ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc
     1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc
     1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt
     1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1381 cttcctttgt cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     1501 tgcgtcgccg cgccgccgtc cccttctcca tctccagcct cggggctgcc gcagggggac
     1561 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1621 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1681 gtgctggtta ttgtgctgtc tcatcatttt ggcaaaggcc gccaccatga gcgagctgat
     1741 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     1801 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     1861 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     1921 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     1981 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     2041 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     2101 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     2161 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     2221 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     2281 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     2341 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     2401 tagcaaactg gggcacaagc ttaattaaca ctcctcaggt gcaggctgcc tatcagaagg
     2461 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     2521 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     2581 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     2641 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     2701 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
     2761 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2821 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2881 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2941 ttatggagat ccctcgacct gcagcccaag cttggatccc ctaggtttct gatcctgtgt
     3001 agagacccta gtttgaatgt gcttatagcc tattattaca tgtgtaatgt tacataaatt
     3061 acttaactcg gatttttaat ttcatcagct atttaaaatg ggcataatat aactatatta
     3121 aatggctgtt atgaagatta aataagatga tatgtaaaat gtgttttttg tttgtttgtt
     3181 tgtttgtctg tttgtttttt tgagacagag tcttgctctg ttacccaggc tggagtgcag
     3241 tggcacaatc ttggctcact gcaagttcta cctcccgagt tcatgccatt ctcctgcctc
     3301 agcccctccc aagtagctgg gactacaggc acccgccacc acgcctggct aattttttgt
     3361 atttttggta gagatggggt ttcaccatat tagccaggat ggtctcgatc tcctgacctc
     3421 gtgatctgcc cacctcggcc tcccaaattg ctgggattac aggcatgagc cactgcgccc
     3481 agcctaaaat gtttttttac ataatgggtg ttcagcacat gttaaagcct tctctccatc
     3541 cttcttccct tttgtttcat gggttgactg atctgtctct agtgctgtac ttttaaagct
     3601 tctacagttc tgaattcaaa attatcttct cactgggccc cggtgttatc tcattctttt
     3661 ttctcctctg taagttgaca tgtgatgtgg gaacaaaggg gataaagtca ttattttgtg
     3721 ctaaaatcgt aattggagag gacctcctgt tagctgggct ttcttctatt tattgtggtg
     3781 gttactggag ttccttcttc tagttttagg atatatatat atattttttt ctttccctga
     3841 agatataata atatatatac ttctgaagat tgagattttt aaattagttg tattgaaaac
     3901 tagctaatca gcaatttaag gctagcttga gacttatgtc ttgaatttgt ttttgtaggc
     3961 tccaaaacca aggagggagt ggtgcatggt gtggcaacag gtaagctcca ttgtgcttat
     4021 atcgaaagat gatatttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
     4081 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
     4141 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
     4201 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
     4261 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
     4321 atatatatat tttcttgtta tagatatcaa ctagaatgct agttattaat agtaatcaat
     4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4681 tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca
     4741 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     4801 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgcgccaggc
     4861 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     4921 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     4981 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc
     5041 cgcgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     5101 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt
     5161 tcttttctgt ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg
     5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg
     5281 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc
     5341 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa
     5401 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg
     5461 gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     5521 cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     5581 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     5641 gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     5701 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct
     5761 gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga
     5821 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc
     5881 agcctcgggg ctgccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     5941 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     6001 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     6061 aggccgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
     6121 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     6181 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     6241 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     6301 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     6361 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     6421 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     6481 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     6541 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     6601 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     6661 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     6721 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aaggagggca
     6781 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctacc gagtacaagc
     6841 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     6901 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     6961 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7021 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7081 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7141 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7201 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7261 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7321 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     7381 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacactcc tcaggtgcag
     7441 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     7501 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     7561 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     7621 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     7681 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     7741 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     7801 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     7861 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     7921 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggatccctc
     7981 gacttttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa
     8041 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact
     8101 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc
     8161 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaaa
     8221 gtatctagtg gttagtgtgg cccagtattc aagattccta tgaaattgta aaacaatcac
     8281 tgagcattct aagaacatat cagtcttatt gaaactgaat tctttataaa gtatttttaa
     8341 ataggtaaat attgattata aataaaaaat atacttgcca agaataatga gggctttgaa
     8401 ttgataagct atgtttaatt tatagtaagt gggcatttaa atattctgac caaaaatgta
     8461 ttgacaaact gctgacaaaa ataaaatgtg aatattgcca taattttaaa aaaagtaaaa
     8521 tttctgttga ttacagtaaa atattttgac cttaaattat gttgattaca atattccttt
     8581 gataattcag agtgcatttc aggaaacacc cttggacagt cagtaaattg tttattgtat
     8641 ttatctttgt attgttatgg tatagctatt tgtacaaata ttattgtgca attattacat
     8701 ttctgattat attattcatt tggcctaaat ttaccgagaa tttgaacaag tcaattaggt
     8761 ttataatcaa gaaatatcaa aaatgatgaa aaggatgata atcatcatca gatgttgagg
     8821 aagatgagga tgagagtgcc agaaatagag aaatcaaagg agaaccaaaa tttaacaaat
     8881 taaaagccca cagacttgct gtaattaagt tttctgttgt aagtactcca cgtttcctgg
     8941 cagatgtggt gaagcaaaag atataatcag aaatataatt tatatgatcg gaaagcatta
     9001 aacacaatag tgcctataca aataaaatgt tcctatcact gacttctaaa atggaaatga
     9061 ggacaatgat atgggaatct taatacagtg ttgtggatag gactaaaaac acaggagtca
     9121 gatcttcttg gttcaacttc ctgcttactc cttaccagct gtgtgaacct agtatacgcg
     9181 tatgcggccg cttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     9241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gcttaaaact
     9481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9721 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     9781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10201 caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc
    10261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagaatcaat
    10381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11401 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
    11461 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
    11521 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
    11581 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
    11641 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
    11701 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
    11761 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
    11821 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
    11881 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    11941 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    12001 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggggcgc
    12061 gccatt
//
