LOCUS       sequence-197517-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9, REC3 FRET variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC052 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1003..1026
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1084..1104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1111..5217
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDCLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKCKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5343..5361)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5357..5404
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(5770..5775,5776..5792,5793..5798))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(5825..5843)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5911..6015
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6016..6084,6085..6876)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6234..6253)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7047..7635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7536..7555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7789..7806
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7821..7960
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7886..7905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8046..8068
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8062..8253)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     9711..9730
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9814..9833
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9814..9832
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             9921..9943
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9963..9992
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(10002..10073,1..1029)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
ORIGIN
        1 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
       61 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca
      121 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
      181 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
      241 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
      301 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
      361 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
      421 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg
      481 gataacgctg gcgcaaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac
      541 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
      601 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
      661 gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc
      721 gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat
      781 ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg
      841 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac
      901 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
      961 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     1021 gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc
     1081 gaggaaaacc tgtacttcca atccaatgca atggataaga aatactcaat aggcttagat
     1141 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
     1201 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
     1261 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
     1321 aggtatacac gtcggaagaa tcgtatttct tatctacagg agattttttc aaatgagatg
     1381 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
     1441 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
     1501 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat
     1561 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
     1621 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
     1681 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
     1741 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
     1801 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
     1861 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
     1921 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
     1981 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
     2041 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
     2101 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
     2161 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
     2221 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
     2281 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
     2341 ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
     2401 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
     2461 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
     2521 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
     2581 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
     2641 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
     2701 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
     2761 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
     2821 aaaatagaat cttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
     2881 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
     2941 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     3001 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
     3061 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
     3121 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
     3181 cgcaatttta tgcagctgat ccatgatgat tgtttgacat ttaaagaaga cattcaaaaa
     3241 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
     3301 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
     3361 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
     3421 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     3481 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     3541 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     3601 aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat
     3661 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     3721 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     3781 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     3841 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     3901 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     3961 cgagaggtta aagtgattac cttaaaatgt aaattagttt ctgacttccg aaaagatttc
     4021 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     4081 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     4141 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     4201 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     4261 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     4321 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     4381 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
     4441 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     4501 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     4561 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
     4621 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     4681 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     4741 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     4801 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     4861 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     4921 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     4981 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     5041 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     5101 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     5161 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgactaataa
     5221 cattggaagt ggataacgga tccgcgatcg cggcgcgcca cctggtggcc ggccggtacc
     5281 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     5341 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     5401 tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg
     5461 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt
     5521 cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca
     5581 gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca
     5641 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
     5701 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
     5761 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     5821 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5881 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5941 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataacat
     6001 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6061 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6121 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6181 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6241 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     6301 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6361 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6421 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6481 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6541 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6601 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6661 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6721 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6781 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6841 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6901 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6961 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7681 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7741 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7801 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7861 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7921 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     7981 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8041 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     8101 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     8161 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     8221 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     8281 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     8341 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     8401 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     8461 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     8521 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     8581 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     8641 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     8701 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct
     8761 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     8821 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     8881 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     8941 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     9001 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc
     9061 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     9121 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     9181 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg
     9241 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     9301 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     9361 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     9421 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     9481 ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc
     9541 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     9601 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     9661 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     9721 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     9781 gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata gggagaccac
     9841 aacggtttcc ctctagtgcc ggctccggag agctctttaa ttaagcggcc gccctgcagg
     9901 actcgagttc tagaaataat tttgtttaac tttaagaagg agatatacat atgaaatctt
     9961 ctcaccatca ccatcaccat caccatcacc atggttcttc tatgaaaatc gaagaaggta
    10021 aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa gtc
//
