LOCUS       sequence-197518-       12082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and 
            tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 12082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe3-A53T
FEATURES             Location/Qualifiers
     source          1..12082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        75..378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..659
                     /label=chicken beta-actin promoter
     intron          660..1668
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1727..2428
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    2572..2627
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   4040..4074
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        4420..4723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4726..5004
                     /label=chicken beta-actin promoter
     intron          5005..6013
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     regulatory      6066..6075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6072..6074,6075..6077,6078..6788)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6072..6074,6075..6077,6078..6788)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6117..6138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6378..6397)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6725..6746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6789..6842
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6843..7439
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7336..7356
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7583..7638
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   8168..8230
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9230..9250)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9230..9248)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9269..9285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9269..9285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9282..9304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9293..9309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9317..9323,9324..9341,9342..9347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9362..9383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      9620..10208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10109..10128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10362..10379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(join(10430..11221,11222..11290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11053..11072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11291..11395)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11421..11876)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11558..11579)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11770..11789
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12003..12025
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12017..12034
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12018..12034
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12044..12063
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12044..12062
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
       61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      361 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
      421 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
      481 gggggcgggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc
      721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      781 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      841 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg
      961 ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg
     1021 tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1081 ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc
     1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc
     1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt
     1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1381 cttcctttgt cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     1501 tgcgtcgccg cgccgccgtc cccttctcca tctccagcct cggggctgcc gcagggggac
     1561 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1621 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1681 gtgctggtta ttgtgctgtc tcatcatttt ggcaaaggcc gccaccatga gcgagctgat
     1741 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     1801 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     1861 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     1921 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     1981 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     2041 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     2101 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     2161 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     2221 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     2281 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     2341 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     2401 tagcaaactg gggcacaagc ttaattaaca ctcctcaggt gcaggctgcc tatcagaagg
     2461 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     2521 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     2581 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     2641 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     2701 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
     2761 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2821 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2881 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2941 ttatggagat ccctcgacct gcagcccaag cttggatccc ctaggtttct gatcctgtgt
     3001 agagacccta gtttgaatgt gcttatagcc tattattaca tgtgtaatgt tacataaatt
     3061 acttaactcg gatttttaat ttcatcagct atttaaaatg ggcataatat aactatatta
     3121 aatggctgtt atgaagatta aataagatga tatgtaaaat gtgttttttg tttgtttgtt
     3181 tgtttgtctg tttgtttttt tgagacagag tcttgctctg ttacccaggc tggagtgcag
     3241 tggcacaatc ttggctcact gcaagttcta cctcccgagt tcatgccatt ctcctgcctc
     3301 agcccctccc aagtagctgg gactacaggc acccgccacc acgcctggct aattttttgt
     3361 atttttggta gagatggggt ttcaccatat tagccaggat ggtctcgatc tcctgacctc
     3421 gtgatctgcc cacctcggcc tcccaaattg ctgggattac aggcatgagc cactgcgccc
     3481 agcctaaaat gtttttttac ataatgggtg ttcagcacat gttaaagcct tctctccatc
     3541 cttcttccct tttgtttcat gggttgactg atctgtctct agtgctgtac ttttaaagct
     3601 tctacagttc tgaattcaaa attatcttct cactgggccc cggtgttatc tcattctttt
     3661 ttctcctctg taagttgaca tgtgatgtgg gaacaaaggg gataaagtca ttattttgtg
     3721 ctaaaatcgt aattggagag gacctcctgt tagctgggct ttcttctatt tattgtggtg
     3781 gttactggag ttccttcttc tagttttagg atatatatat atattttttt ctttccctga
     3841 agatataata atatatatac ttctgaagat tgagattttt aaattagttg tattgaaaac
     3901 tagctaatca gcaatttaag gctagcttga gacttatgtc ttgaatttgt ttttgtaggc
     3961 tccaaaacca aggagggagt ggtgcatggt gtgacaacag gtaagctcca ttgtgcttat
     4021 atcgaaagat gatatttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
     4081 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
     4141 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
     4201 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
     4261 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
     4321 atatatatat tttcttgtta tagatatcaa ctagaatgct agttattaat agtaatcaat
     4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4681 tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca
     4741 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     4801 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgcgccaggc
     4861 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     4921 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     4981 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc
     5041 cgcgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     5101 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt
     5161 tcttttctgt ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg
     5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg
     5281 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc
     5341 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa
     5401 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg
     5461 gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     5521 cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     5581 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     5641 gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     5701 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct
     5761 gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga
     5821 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc
     5881 agcctcgggg ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt
     5941 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     6001 ctttttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa
     6061 aggccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     6121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6241 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6481 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     6541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     6601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6661 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6721 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6781 tgtacaagga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     6841 ctaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     6901 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     6961 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     7021 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7081 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7141 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7201 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7261 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7321 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7381 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     7441 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     7501 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     7561 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     7621 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     7681 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     7741 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     7801 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     7861 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     7921 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     7981 aagcttggat ccctcgactt ttgttacttt atagaagaaa ttttgagttt ttgttttttt
     8041 ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt
     8101 aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga
     8161 taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat
     8221 ctttctaggg ttaaagtatc tagtgattag tgtggcccag tattcaagat tcctatgaaa
     8281 ttgtaaaaca atcactgagc attctaagaa catatcagtc ttattgaaac tgaattcttt
     8341 ataaagtatt tttaaatagg taaatattga ttataaataa aaaatatact tgccaagaat
     8401 aatgagggct ttgaattgat aagctatgtt taatttatag taagtgggca tttaaatatt
     8461 ctgaccaaaa atgtattgac aaactgctga caaaaataaa atgtgaatat tgccataatt
     8521 ttaaaaaaag taaaatttct gttgattaca gtaaaatatt ttgaccttaa attatgttga
     8581 ttacaatatt cctttgataa ttcagagtgc atttcaggaa acacccttgg acagtcagta
     8641 aattgtttat tgtatttatc tttgtattgt tatggtatag ctatttgtac aaatattatt
     8701 gtgcaattat tacatttctg attatattat tcatttggcc taaatttacc gagaatttga
     8761 acaagtcaat taggtttata atcaagaaat atcaaaaatg atgaaaagga tgataatcat
     8821 catcagatgt tgaggaagat gaggatgaga gtgccagaaa tagagaaatc aaaggagaac
     8881 caaaatttaa caaattaaaa gcccacagac ttgctgtaat taagttttct gttgtaagta
     8941 ctccacgttt cctggcagat gtggtgaagc aaaagatata atcagaaata taatttatat
     9001 gatcggaaag cattaaacac aatagtgcct atacaaataa aatgttccta tcactgactt
     9061 ctaaaatgga aatgaggaca atgatatggg aatcttaata cagtgttgtg gataggacta
     9121 aaaacacagg agtcagatct tcttggttca acttcctgct tactccttac cagctgtgtg
     9181 taacctagta tacgcgtatg cggccgctta attaatccag cttttgttcc ctttagtgag
     9241 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9481 ttgggcgctt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     9781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg
    10261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10321 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10381 aagcggaaga atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11401 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
    11461 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
    11521 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
    11581 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
    11641 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
    11701 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
    11761 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
    11821 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
    11881 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    11941 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    12001 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
    12061 gggcgaattg gggcgcgcca tt
//
