LOCUS       sequence-197520-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC272 - Bacterial expression plasmid for SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     801..824
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             882..902
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             909..5015
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIARQLAETAAITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5141..5159)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5155..5202
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(5568..5573,5574..5590,5591..5596))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(5623..5641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5709..5813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5814..5882,5883..6674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6032..6051)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6845..7433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7334..7353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7587..7604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7619..7758
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7684..7703)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7844..7866
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7860..8051)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     9509..9528
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9612..9631
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9612..9630
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             9719..9741
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9761..9790
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(9800..10073,1..827)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
ORIGIN
        1 cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg
       61 aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag
      121 agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc
      181 tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt
      241 atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg
      301 gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact
      361 ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg
      421 catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca
      481 agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc
      541 cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg
      601 aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt
      661 tggcgaaaga tccacgtatt gccgccacta tggaaaacgc ccagaaaggt gaaatcatgc
      721 cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg atcaacgccg
      781 ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactaat tcgagctcga
      841 acaacaacaa caataacaat aacaacaacc tcgggatcga ggaaaacctg tacttccaat
      901 ccaatgcaat ggataagaaa tactcaatag gcttagatat cggcacaaat agcgtcggat
      961 gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata
     1021 cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga
     1081 cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc
     1141 gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct
     1201 ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta
     1261 tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc
     1321 tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct
     1381 tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata
     1441 atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag
     1501 aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta
     1561 aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat
     1621 ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt
     1681 tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat ttagataatt
     1741 tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag aatttatcag
     1801 atgctatttt actttcagat atcctaagag taaatactga aataactaag gctcccctat
     1861 cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt ttaaaagctt
     1921 tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg
     1981 gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa tttatcaaac
     2041 caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat cgtgaagatt
     2101 tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt cacttgggtg
     2161 agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa gacaatcgtg
     2221 agaagattga aaaaatcttg acttttcgaa ttccttatta tgttggtcca ttggcgcgtg
     2281 gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc ccatggaatt
     2341 ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc atgacaaact
     2401 ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt tatgagtatt
     2461 ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg cgaaaaccag
     2521 catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa acaaatcgaa
     2581 aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatgt tttgatagtg
     2641 ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac catgatttgc
     2701 taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat atcttagagg
     2761 atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa agacttaaaa
     2821 catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc cgttatactg
     2881 gttggggacg tttgtctcga aaattgatta atggtattag ggataagcaa tctggcaaaa
     2941 caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg cagctgatcc
     3001 atgatgatag tttgacattt aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg
     3061 atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa aaaggtattt
     3121 tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat aagccagaaa
     3181 atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag aaaaattcgc
     3241 gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag attcttaaag
     3301 agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat tatctccaaa
     3361 atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt gattatgatg
     3421 tcgatcacat tgttccacaa agtttcctta aagacgattc aatagacaat aaggtcttaa
     3481 cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa
     3541 agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa cgtaagtttg
     3601 ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct ggttttatcg
     3661 ctcgccaatt ggctgaaact gccgctatca ctaagcatgt ggcacaaatt ttggatagtc
     3721 gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa gtgattacct
     3781 taaaatctaa attagtttct gacttccgaa aagatttcca attctataaa gtacgtgaga
     3841 ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga actgctttga
     3901 ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa gtttatgatg
     3961 ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca aaatatttct
     4021 tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat ggagagattc
     4081 gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg gataaagggc
     4141 gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa
     4201 cagaagtaca gacaggcgga ttctccaagg agtcaatttt accaaaaaga aattcggaca
     4261 agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt gatagtccaa
     4321 cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg aagaagttaa
     4381 aatccgttaa agagttacta gggatcacaa ttatggaaag aagttccttt gaaaaaaatc
     4441 cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta atcattaaac
     4501 tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg gctagtgccg
     4561 gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat tttttatatt
     4621 tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa aaacaattgt
     4681 ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt gaattttcta
     4741 agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat aacaaacata
     4801 gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg ttgacgaatc
     4861 ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa cgatatacgt
     4921 ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt ctttatgaaa
     4981 cacgcattga tttgagtcag ctaggaggtg actaataaca ttggaagtgg ataacggatc
     5041 cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc tgatccggct
     5101 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     5221 tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag
     5281 tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg
     5341 tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat
     5401 gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat
     5461 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
     5521 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc
     5581 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
     5641 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5701 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5761 tcatgagaca ataaccctga taaatgcttc aataacattg aaaaaggaag agtatgagta
     5821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5941 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6001 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     6061 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6121 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6181 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6241 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6301 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     6361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6961 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7621 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
     7681 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     7741 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7801 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7861 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     7921 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     7981 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     8041 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     8101 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     8161 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     8221 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     8281 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     8341 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     8401 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     8461 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
     8521 caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     8581 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
     8641 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
     8701 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc
     8761 ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga
     8821 tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
     8881 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
     8941 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
     9001 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
     9061 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
     9121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
     9181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
     9241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     9301 agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     9361 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     9421 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     9481 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     9541 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
     9601 tcccgcgaaa ttaatacgac tcactatagg gagaccacaa cggtttccct ctagtgccgg
     9661 ctccggagag ctctttaatt aagcggccgc cctgcaggac tcgagttcta gaaataattt
     9721 tgtttaactt taagaaggag atatacatat gaaatcttct caccatcacc atcaccatca
     9781 ccatcaccat ggttcttcta tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
     9841 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
     9901 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
     9961 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
    10021 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atc
//
