LOCUS       sequence-197528-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging. Plant expression of RFP-RabG3B-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBI10:RFP-RabG3B-WT
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    799..939
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(865..884)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1025..1047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1283..1477
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1543..2616)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3045..3674)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5005..5029
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    5194..5215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5230..5235,5236..5253,5254..5260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5268..5284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5273..5295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5292..5308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5292..5308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5996..6670
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             5996..6670
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     6394..6413
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6588..6611
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    6683..6707
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7323..7347)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7717..7896
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7938..8489
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      8509..8761
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8854..8878
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9399..10190
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      841 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
      901 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
      961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     1081 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     1141 tagattgcct ggccctaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     1201 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     1261 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     1321 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     1381 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     1441 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     1501 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     1561 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     1621 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     1681 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     1741 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
     1801 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
     1861 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
     1921 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
     1981 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
     2041 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
     2101 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
     2161 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
     2221 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
     2281 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
     2341 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
     2401 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
     2461 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
     2521 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
     2581 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
     2641 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
     2701 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
     2761 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
     2821 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
     2881 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
     2941 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
     3001 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
     3061 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
     3121 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
     3181 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
     3241 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
     3301 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
     3361 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
     3421 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
     3481 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
     3541 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
     3601 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
     3661 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
     3721 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
     3781 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
     3841 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
     3901 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
     3961 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
     4021 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
     4081 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
     4141 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
     4201 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
     4261 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
     4321 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
     4381 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
     4441 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
     4501 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
     4561 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
     4621 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
     4681 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
     4741 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
     4801 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
     4861 ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca
     4921 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
     4981 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
     5041 tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctgctct agccaatacg
     5101 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5161 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5221 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5281 acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgacga
     5341 gtcagtaata aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac
     5401 tcaaagcaca aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg
     5461 cgtgtaaaca acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta
     5521 gctttctcgt gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc
     5581 aaagagctct tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc
     5641 tcaattctct ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc
     5701 gattttgttt tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc
     5761 ttagatcgaa gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg
     5821 gtttcataga tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt
     5881 cgatttgtga tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgta
     5941 gattaatctg agtttttctg attaacactc gagtgcggga tcctctagaa ctagtatggc
     6001 ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt
     6061 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     6121 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     6181 ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     6241 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     6301 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     6361 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     6421 ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     6481 gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat
     6541 ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac
     6601 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc
     6661 caccggcgcc caattgttaa tcacaagttt gtacaaaaaa gcaggcttca tgtcgacgcg
     6721 aagacgaact ttactcaaag ttataattct tggagacagc ggggttggca aaacatcgtt
     6781 gatgaatcaa tatgtgaata acaagtttag tcaacagtac aaagctacga tcggagctga
     6841 ttttgtcact aaggagcttc aaattgatga caggcttgtc acattgcaaa tatgggacac
     6901 tgctgggcaa gagaggtttc aaagtcttgg tgttgctttc tatagaggtg cagattgttg
     6961 tgttcttgtc tatgatgtga atcacttgaa gtcatttgaa tctctcgaca attggcacaa
     7021 cgagtttctt acacgggcta gtccacgtga cccaatggca ttccctttta tacttcttgg
     7081 taataaggtt gatattgatg gaggaaatag ccgagtggta tctgagaaga aggctagaga
     7141 atggtgtgct gaaaagggaa acatagtcta tttcgagaca tcggctaaag aagattacaa
     7201 tgtcgatgac tccttcttgt gcatcacaaa acttgccctt gcaaatgaac gcgaccaaga
     7261 tatatatttc cagccagata ctggttcggt gcctgagcaa agaggaggtt gtgcttgctg
     7321 agacccagct ttcttgtaca aagtggtgat taagcggcca tgctagagtc cgcaaaaatc
     7381 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     7441 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     7501 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     7561 aaaccaaaat ccagtgacct gcaggcatgc gacgtcgggc cctctagagg atccccgggt
     7621 accgcgaatt atcgatcatg agcggagaat taagggagtc acgttatgac ccccgccgat
     7681 gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac
     7741 tgagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg
     7801 cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc
     7861 actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca ctctcaactc
     7921 gatcgagggg atctaccatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg
     7981 aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca
     8041 acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg
     8101 agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg
     8161 gtccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc
     8221 cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg
     8281 aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc
     8341 gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc
     8401 acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtgccgcccc
     8461 gtccggtcct gcccgtcacc gaaatctgat gacccctaga gtcaagcaga tcgttcaaac
     8521 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     8581 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     8641 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     8701 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     8761 cgaccggcat gcaagctgat aattcaattc ggcgttaatt cagtacatta aaaacgtccg
     8821 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     8881 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     8941 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     9001 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     9061 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc
     9121 gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     9181 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     9241 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     9301 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     9361 cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg
     9421 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     9481 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     9541 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     9601 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     9661 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     9721 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     9781 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     9841 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     9901 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     9961 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
    10021 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
    10081 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
    10141 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta
    10201 gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
    10261 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
    10321 gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac
    10381 gtgagttttc gttccactga gcgtcagacc c
//
