LOCUS       sequence-197537-        6243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent tagging. Plant expression of YFPc-RabG3b-WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6243)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 35S:YFPc-RabG3b-WT
FEATURES             Location/Qualifiers
     source          1..6243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      13..601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     502..521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    913..937
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1031..1283)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1303..1854)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1896..2075)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2544..2888
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2832..2852
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2890..2906
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2891..2907
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2922..3173
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3110..3131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3186..3210
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3826..3850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3860..3886
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3899..3922
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4215..4239
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4351..5060
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5133..5224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5225..5293,5294..6085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5443..5462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
       61 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      121 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      661 ggactctagc tagaggatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      721 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      781 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      841 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      901 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
      961 taacacattg cggacgtttt taatgatcga atactaacgt ctctaccaga tatcagcttg
     1021 catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     1081 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     1141 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     1201 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     1261 tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt cggtgacggg
     1321 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
     1381 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
     1441 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
     1501 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
     1561 ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc
     1621 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
     1681 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
     1741 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
     1801 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga
     1861 tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1921 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1981 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     2041 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     2101 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcag
     2161 attgtcgttt cccgccttcg gtttgggcgc gcccggttca gaagaccaga gggctattga
     2221 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     2281 tcacttcatc gaaaggacag tagaaaagga agatggcttc tacaaatgcc atcattgcga
     2341 taaaggaaag gctatcgttc aagatgcctc taccgacagt ggtcccaaag atggaccccc
     2401 acccacgagg aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2461 ttgatgtgat acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     2521 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     2581 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat
     2641 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc
     2701 gacagtggtc ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt
     2761 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     2821 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     2881 gagaggacct cgaggtcgac ggtatcgata agcttataat ggacaagcag aagaacggca
     2941 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     3001 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3061 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3121 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggagtcc
     3181 ccatcacaag tttgtacaaa aaagcaggct tcatgtcgac gcgaagacga actttactca
     3241 aagttataat tcttggagac agcggggttg gcaaaacatc gttgatgaat caatatgtga
     3301 ataacaagtt tagtcaacag tacaaagcta cgatcggagc tgattttgtc actaaggagc
     3361 ttcaaattga tgacaggctt gtcacattgc aaatatggga cactgctggg caagagaggt
     3421 ttcaaagtct tggtgttgct ttctatagag gtgcagattg ttgtgttctt gtctatgatg
     3481 tgaatcactt gaagtcattt gaatctctcg acaattggca caacgagttt cttacacggg
     3541 ctagtccacg tgacccaatg gcattccctt ttatacttct tggtaataag gttgatattg
     3601 atggaggaaa tagccgagtg gtatctgaga agaaggctag agaatggtgt gctgaaaagg
     3661 gaaacatagt ctatttcgag acatcggcta aagaagatta caatgtcgat gactccttct
     3721 tgtgcatcac aaaacttgcc cttgcaaatg aacgcgacca agatatatat ttccagccag
     3781 atactggttc ggtgcctgag caaagaggag gttgtgcttg ctgagaccca gctttcttgt
     3841 acaaagtggt gatgggggtt atccatacga tgttccagat tatgctgtcg gcgccggttg
     3901 gtctcatcct caatttgaaa aataagtcta gagtccgcaa aaatcaccag tctctctcta
     3961 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
     4021 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     4081 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     4141 gaccgggcgg ccgccaccgc ggtggagggg gatcagattg tcgtttcccg ccttcagttt
     4201 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     4261 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     4321 gtacatcacc gacgagcaag gcaagaccga gcgcctttcc gacgctcacc gggctggttg
     4381 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
     4441 agccgtgtgc gagacaccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     4501 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     4561 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     4621 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     4681 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     4741 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     4801 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     4861 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     4921 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     4981 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
     5041 cctcacccca aaaatggcag cgctggcagt ccttgccatt gccgggatcg gggcagtaac
     5101 gggatgggcg atcagcccga aagctacccc tatttgttta tttttctaaa tacattcaaa
     5161 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5221 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5281 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5341 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5401 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5461 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5521 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5581 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5641 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5701 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5761 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5821 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5881 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5941 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6001 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6061 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6121 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6181 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6241 gat
//
