LOCUS       sequence-197545-        9847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson
            CL, Kiguchi K, Walker CL
  TITLE     Activity of TSC2 is inhibited by AKT-mediated phosphorylation and 
            membrane partitioning.
  JOURNAL   J Cell Biol. 2006 Apr 24. 173(2):279-89.
  PUBMED    16636147
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2006 Apr 24. 173(2):279-89."
COMMENT     Sequence Label: Flag TSC2 wt
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6276..6292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6277..6293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6348..6367)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6349..6367)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6641..6762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6678..6697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6732..6751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6769..7224)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6906..6927)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7118..7137
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7251..7355
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7357..7714
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7357..7548
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7380..7400)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7565..7700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7749..8543
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7749..8543
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7803..7822)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8413..8432
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8731..8750)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8775..8822
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9151..9739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9640..9659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagaagcc cgggcggatc
      721 ccatcgaatt ccggtgcgtc ctggtccacc atggccaaac caacaagcaa agattcaggc
      781 ttgaaggaga agtttaagat tctgttggga ctgggaacac cgaggccaaa tcccaggtct
      841 gcagagggta aacagacgga gtttatcatc accgcggaaa tactgagaga actgagcatg
      901 gaatgtggcc tcaacaatcg catccggatg atagggcaga tttgtgaagt cgcaaaaacc
      961 aagaaatttg aagagcacgc agtggaagca ctctggaagg cggtcgcgga tctgttgcag
     1021 ccggagcggc cgctggaggc ccggcacgcg gtgctggctc tgctgaaggc catcgtgcag
     1081 gggcagggcg agcgtttggg ggtcctcaga gccctcttct ttaaggtcat caaggattac
     1141 ccttccaacg aagaccttca cgaaaggctg gaggttttca aggccctcac agacaatggg
     1201 agacacatca cctacttgga ggaagagctg gctgactttg tcctgcagtg gatggatgtt
     1261 ggcttgtcct cggaattcct tctggtgctg gtgaacttgg tcaaattcaa tagctgttac
     1321 ctcgacgagt acatcgcaag gatggttcag atgatctgtc tgctgtgcgt ccggaccgcg
     1381 tcctctgtgg acatagaggt ctccctgcag gtgctggacg ccgtggtctg ctacaactgc
     1441 ctgccggctg agagcctccc gctgttcatc gttaccctct gtcgcaccat caacgtcaag
     1501 gagctctgcg agccttgctg gaagctgatg cggaacctcc ttggcaccca cctgggccac
     1561 agcgccatct acaacatgtg ccacctcatg gaggacagag cctacatgga ggacgcgccc
     1621 ctgctgagag gagccgtgtt ttttgtgggc atggctctct ggggagccca ccggctctat
     1681 tctctcagga actcgccgac atctgtgttg ccatcatttt accaggccat ggcatgtccg
     1741 aacgaggtgg tgtcctatga gatcgtcctg tccatcacca ggctcatcaa gaagtatagg
     1801 aaggagctcc aggtggtggc gtgggacatt ctgctgaaca tcatcgaacg gctccttcag
     1861 cagctccaga ccttggacag cccggagctc aggaccatcg tccatgacct gttgaccacg
     1921 gtggaggagc tgtgtgacca gaacgagttc cacgggtctc aggagagata ctttgaactg
     1981 gtggagagat gtgcggacca gaggcctgag tcctccctcc tgaacctgat ctcctataga
     2041 gcgcagtcca tccacccggc caaggacggc tggattcaga acctgcaggc gctgatggag
     2101 agattcttca ggagcgagtc ccgaggcgcc gtgcgcatca aggtgctgga cgtgctgtcc
     2161 tttgtgctgc tcatcaacag gcagttctat gaggaggagc tgattaactc agtggtcatc
     2221 tcgcagctct cccacatccc cgaggataaa gaccaccagg tccgaaagct ggccacccag
     2281 ttgctggtgg acctggcaga gggctgccac acacaccact tcaacagcct gctggacatc
     2341 atcgagaagg tgatggcccg ctccctctcc ccacccccgg agctggaaga aagggatgtg
     2401 gccgcatact cggcctcctt ggaggatgtg aagacagccg tcctggggct tctggtcatc
     2461 cttcagacca agctgtacac cctgcctgca agccacgcca cgcgtgtgta tgagatgctg
     2521 gtcagccaca ttcagctcca ctacaagcac agctacaccc tgccaatcgc gagcagcatc
     2581 cggctgcagg cctttgactt cctgttgctg ctgcgggccg actcactgca ccgcctgggc
     2641 ctgcccaaca aggatggagt cgtgcggttc agcccctact gcgtctgcga ctacatggag
     2701 ccagagagag gctctgagaa gaagaccagc ggcccccttt ctcctcccac agggcctcct
     2761 ggcccggcgc ctgcaggccc cgccgtgcgg ctggggtccg tgccctactc cctgctcttc
     2821 cgcgtcctgc tgcagtgctt gaagcaggag tctgactgga aggtgctgaa gctggttctg
     2881 ggcaggctgc ctgagtccct gcgctataaa gtgctcatct ttacttcccc ttgcagtgtg
     2941 gaccagctgt gctctgctct ctgctccatg ctttcaggcc caaagacact ggagcggctc
     3001 cgaggcgccc cagaaggctt ctccagaact gacttgcacc tggccgtggt tccagtgctg
     3061 acagcattaa tctcttacca taactacctg gacaaaacca aacagcgcga gatggtctac
     3121 tgcctggagc agggcctcat ccaccgctgt gccagacagt gcgtcgtggc cttgtccatc
     3181 tgcagcgtgg agatgcctga catcatcatc aaggcgctgc ctgttctggt ggtgaagctc
     3241 acgcacatct cagccacagc cagcatggcc gtcccactgc tggagttcct gtccactctg
     3301 gccaggctgc cgcacctcta caggaacttt gccgcggagc agtatgccag tgtgttcgcc
     3361 atctccctgc cgtacaccaa cccctccaag tttaatcagt acatcgtgtg tctggcccat
     3421 cacgtcatag ccatgtggtt catcaggtgc cgcctgccct tccggaagga ttttgtccct
     3481 ttcatcacta agggcctgcg gtccaatgtc ctcttgtctt ttgatgacac ccccgagaag
     3541 gacagcttca gggcccggag tactagtctc aacgagagac ccaagagtct gaggatagcc
     3601 agacccccca aacaaggctt gaataactct ccacccgtga aagaattcaa ggagagctct
     3661 gcagccgagg ccttccggtg ccgcagcatc agtgtgtctg aacatgtggt ccgcagcagg
     3721 atacagacgt ccctcaccag tgccagcttg gggtctgcag atgagaactc cgtggcccag
     3781 gctgacgata gcctgaaaaa cctccacctg gagctcacgg aaacctgtct ggacatgatg
     3841 gctcgatacg tcttctccaa cttcacggct gtcccgaaga ggtctcctgt gggcgagttc
     3901 ctcctagcgg gtggcaggac caaaacctgg ctggttggga acaagcttgt cactgtgacg
     3961 acaagcgtgg gaaccgggac ccggtcgtta ctaggcctgg actcggggga gctgcagtcc
     4021 ggcccggagt cgagctccag ccccggggtg catgtaagac agaccaagga ggcgccggcc
     4081 aagctggagt cccaggctgg gcagcaggtg tcccgtgggg cccgggatcg ggtccgttcc
     4141 atgtcggggg gccatggtct tcgagttggc gccctggacg tgccggcctc ccagttcctg
     4201 ggcagtgcca cttctccagg accacggact gcaccagccg cgaaacctga gaaggcctca
     4261 gctggcaccc gggttcctgt gcaggagaag acgaacctgg cggcctatgt gcccctgctg
     4321 acccagggct gggcggagat cctggtccgg aggcccacag ggaacaccag ctggctgatg
     4381 agcctggaga acccgctcag ccctttctcc tcggacatca acaacatgcc cctgcaggag
     4441 ctgtctaacg ccctcatggc ggctgagcgc ttcaaggagc accgggacac agccctgtac
     4501 aagtcactgt cggtgccggc agccagcacg gccaaacccc ctcctctgcc tcgctccaac
     4561 acagactccg ccgtggtcat ggaggaggga agtccgggcg aggttcctgt gctggtggag
     4621 cccccagggt tggaggacgt tgaggcagcg ctaggcatgg acaggcgcac ggatgcctac
     4681 agcaggtcgt cctcagtctc cagccaggag gagaagtcgc tccacgcgga ggagctggtt
     4741 ggcaggggca tccccatcga gcgagtcgtc tcctcggagg gtggccggcc ctctgtggac
     4801 ctctccttcc agccctcgca gcccctgagc aagtccagct cctctcccga gctgcagact
     4861 ctgcaggaca tcctcgggga ccctggggac aaggccgacg tgggccggct gagccctgag
     4921 gttaaggccc ggtcacagtc agggaccctg gacggggaaa gtgctgcctg gtcggcctcg
     4981 ggcgaagaca gtcggggcca gcccgagggt cccttgcctt ccagctcccc ccgctcgccc
     5041 agtggcctcc ggccccgagg ttacaccatc tccgactcgg ccccatcacg caggggcaag
     5101 agagtagaga gggacgcctt aaagagcaga gccacagcct ccaatgcaga gaaagtgcca
     5161 ggcatcaacc ccagtttcgt gttcctgcag ctctaccatt cccccttctt tggcgacgag
     5221 tcaaacaagc caatcctgct gcccaatgag tcacagtcct ttgagcggtc ggtgcagctc
     5281 ctcgaccaga tcccatcata cgacacccac aagatcgccg tcctgtatgt tggagaaggc
     5341 cagagcaaca gcgagctcgc catcctgtcc aatgagcatg gctcctacag gtacacggag
     5401 ttcctgacgg gcctgggccg gctcatcgag ctgaaggact gccagccgga caaggtgtac
     5461 ctgggaggcc tggacgtgtg tggtgaggac ggccagttca cctactgctg gcacgatgac
     5521 atcatgcaag ccgtcttcca catcgccacc ctgatgccca ccaaggacgt ggacaagcac
     5581 cgctgcgaca agaagcgcca cctgggcaac gactttgtgt ccattgtcta caatgactcc
     5641 ggtgaggact tcaagcttgg caccatcaag ggccagttca actttgtcca cgtgatcgtc
     5701 accccgctgg actacgagtg caacctggtg tccctgcagt gcaggaaaga catggagggc
     5761 cttgtggaca ccagcgtggc caagatcgtg tctgaccgca acctgccctt cgtggcccgc
     5821 cagatggccc tgcacgcaaa tatggcctca caggtgcatc atagccgctc caaccccacc
     5881 gatatctacc cctccaagtg gattgcccgg ctccgccaca tcaagcggct ccgccagcgg
     5941 atctgcgagg aagccgccta ctccaacccc agcctacctc tggtgcaccc tccgtcccat
     6001 agcaaagccc ctgcacagac tccagccgag cccacacctg gctatgaggt gggccagcgg
     6061 aagcgcctca tctcctcggt ggaggacttc accgagtttg tgtgaggccg gggccctccc
     6121 tcctgcactg gccttggacg gtattgcctg tcagtgaaat aaataaagtc ctgaccccag
     6181 tgcacagaca tagaggcaca aaaaaaaaaa aaaaaaaagg aattcctgca gcccggggga
     6241 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     6301 cccggtacct taattaatta aggtaccagg taagtgtacc caattcgccc tatagtgagt
     6361 cgtattacaa ttcactcgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     6421 gatccaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     6481 gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat
     6541 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     6601 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     6661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6721 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     6781 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6841 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6901 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     6961 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7021 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7081 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7141 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7201 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7321 gataaatgct tcaataatat tgaaaaagga agaatcctga ggcggaaaga accagctgtg
     7381 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7441 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7501 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7561 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7621 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7681 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     7741 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7801 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7861 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7921 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7981 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8041 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8101 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8161 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8221 tggacgaaga acatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     8281 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8341 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8401 atcaggacat agcgttggct acccgtgata ttgctgaaga acttggcggc gaatgggctg
     8461 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8521 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     8581 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8641 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8701 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     8761 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     8821 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     8881 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     8941 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     9001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9841 taccgcc
//
