LOCUS       sequence-197558-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Oh JH, Chen SJ, Varshavsky A
  TITLE     A reference-based protein degradation assay without global 
            translation inhibitors.
  JOURNAL   J Biol Chem. 2017 Dec 29;292(52):21457-21465. doi: 
            10.1074/jbc.M117.814236. Epub 2017 Nov 9.
  PUBMED    29122887
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.814236"; journalName: "J Biol Chem"; date: 
            "2017-12-29- 29"; volume: "292"; issue: "52"; pages: "21457-21465"
COMMENT     Sequence Label: pJO629
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(94..341)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     307..325
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(351..377)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(384..944)
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             complement(954..977)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accctcacta aagggaanaa aagctggtac cgggcccccc ctcgaggtcg acggtatcga
       61 taagcttgat atcgaattcc tgcagcccgg gccgcaaatt aaagccttcg agcgtcccaa
      121 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
      181 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
      241 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
      301 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgagtta ggcgtaatct
      361 gggacatcgt atgggtaggt accgtctttc ttctcgtaga cttcaaactt atacttgatg
      421 cctttttcct cctggacctc agagaggacg cctgggtatt ctgggagaag tttatatttc
      481 cccaaatcaa tttctgggaa aaacgtgtca ctttcaaatt cctgcatgat ccttgtcaca
      541 aagagtctga ggtggcctgg ttgattcatg gcttcctggt aaacagaact gcctccgact
      601 atccaaacca tgtctacttt acttgccaat tccggttgtt caataagtct taaggcatca
      661 tccaaacttt tggcaagaaa atgagcgcct cgtggtggtt ctttgagttc tctactgaga
      721 actatattaa ttctgtcctt taaaggtcga ttcttctcag gaatggagaa ccaggttttc
      781 ctacccataa tcaccagatt ctgtttacct tccactgaag aggttgtggt cattctttgg
      841 aagtacttga actcgttcct gagcggaggc cagggtaggt ctccgttctt gccaatcccc
      901 atattttggg acacggcgac gatgcagttc aatggtcgaa ccatggnccc acccttgtca
      961 tcgtcgtcct tgtagtccat gaantcg
//
