LOCUS       sequence-197559-       10936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of 
            dCas9; pCDNA3 vector backbone, mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   O'Geen H, Ren C, Nicolet CM, Perez AA, Halmai J, Le VM, Mackay JP, 
            Farnham PJ, Segal DJ
  TITLE     dCas9-based epigenome editing suggests acquisition of histone 
            methylation is not sufficient for target gene repression.
  JOURNAL   Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 
            10.1093/nar/gkx578.
  PUBMED    28973434
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-09-29- 29"; volume: "45"; issue: 
            "17"; pages: "9901-9916"
COMMENT     Sequence Label: SUV[SET]-dCas9
FEATURES             Location/Qualifiers
     source          1..10936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             702..722
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             741..806
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(1908..1934,1935..1937,1938..4421,4422..4427,4428..4493,
                     4494..4496,4497..6011)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1908..1934,1935..1937,1938..4424,4425..4427,
                     4428..6011)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1908..6011
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6087..6134
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6153..6209
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6273..6292)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6317..6365
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6567..6995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6654..6673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6864..6885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7004..7024)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7009..7338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7189..7324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7251..7270
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7405..8199
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7459..7478)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8069..8088
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8375..8496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8412..8431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8466..8485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8545..8561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8545..8561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8558..8580)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8569..8585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8593..8599,8600..8617,8618..8623))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8638..8659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8776..8793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8947..9535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9027..9046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9706..10497,10498..10566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10329..10348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10567..10671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10746..10765)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatcgaaccc ttgccaccat gcccaagaag aagaggaagg
      721 tgggaggctc cggaggaagc gactataagg accacgacgg agactacaag gatcatgata
      781 ttgattacaa agacgatgac gataagggtg gcgggtccgg cggtggatcc ggtaccccac
      841 ggcagaatct caagtgtgtg cgtatcctca agcagttcca caaggactta gaaagggagc
      901 tgctccggcg gcaccaccgg tcaaagaccc cccggcacct ggacccaagc ttggccaact
      961 acctggtgca gaaggccaag cagaggcggg cgctccgtcg ctgggagcag gagctcaatg
     1021 ccaagcgcag ccatctggga cgcatcactg tagagaatga ggtggacctg gacggccctc
     1081 cgcgggcctt cgtgtacatc aatgagtacc gtgttggtga gggcatcacc ctcaaccagg
     1141 tggctgtggg ctgcgagtgc caggactgtc tgtgggcacc cactggaggc tgctgcccgg
     1201 gggcgtcact gcacaagttt gcctacaatg accagggcca ggtgcggctt cgagccgggc
     1261 tgcccatcta cgagtgcaac tcccgctgcc gctgcggcta tgactgccca aatcgtgtgg
     1321 tacagaaggg tatccgatat gacctctgca tcttccgcac ggatgatggg cgtggctggg
     1381 gcgtccgcac cctggagaag attcgcaaga acagcttcgt catggagtac gtgggagaga
     1441 tcattacctc agaggaggca gagcggcggg gccagatcta cgaccgtcag ggcgccacct
     1501 acctctttga cctggactac gtggaggacg tgtacaccgt ggatgccgcc tactatggca
     1561 acatctccca ctttgtcaac cacagttgtg accccaacct gcaggtgtac aacgtcttca
     1621 tagacaacct tgacgagcgg ctgccccgca tcgctttctt tgccacaaga accatccggg
     1681 caggcgagga gctcaccttt gattacaaca tgcaagtgga ccccgtggac atggagagca
     1741 cccgcatgga ctccaacttt ggcctggctg ggctccctgg ctcccctaag aagcgggtcc
     1801 gtattgaatg caagtgtggg actgagtcct gccgcaaata cctcttcagt accggaggga
     1861 gtggtggaag cggcggttct ggtggctcag gcggcagcgg ccggccaatg gacaagaagt
     1921 actccattgg gctcgctatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt
     1981 acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga
     2041 agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca
     2101 aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga
     2161 tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct
     2221 ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg
     2281 aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca
     2341 gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc
     2401 ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct
     2461 ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg
     2521 gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc
     2581 tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt
     2641 cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc
     2701 aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc
     2761 agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata
     2821 ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct
     2881 atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg
     2941 agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg
     3001 gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg
     3061 gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt
     3121 tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc
     3181 ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca
     3241 catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga
     3301 tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg
     3361 gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg
     3421 aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca
     3481 ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga
     3541 agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca
     3601 aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg
     3661 atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg
     3721 acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt
     3781 tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg
     3841 acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa
     3901 aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt
     3961 ccgatggatt tgccaacagg aacttcatgc agttgatcca tgatgactct ctcaccttta
     4021 aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg
     4081 ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg
     4141 atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc
     4201 gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg
     4261 aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc
     4321 agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg
     4381 atcaggaact ggacatcaat cggctctccg actacgacgt ggatgctatc gtgccccagt
     4441 cttttctcaa agatgattct attgataata aagtgttgac aagatccgat aaaaatagag
     4501 ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc
     4561 agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac
     4621 gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac
     4681 gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg
     4741 aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag
     4801 atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc
     4861 atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg
     4921 aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt
     4981 ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt
     5041 ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa
     5101 caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga
     5161 aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct
     5221 tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag
     5281 attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg
     5341 ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg
     5401 gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga
     5461 aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg
     5521 agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg
     5581 agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc
     5641 tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact
     5701 accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg
     5761 ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc
     5821 aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca
     5881 agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg
     5941 ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc
     6001 tcggtggaga cggtggtagt ggaggttcag gaggatccgg ggggagcgga gggagcgcta
     6061 gcggtggagg ctccggaggt ggatctaaaa ggccggcggc cacgaaaaag gccggtcagg
     6121 caaaaaagaa aaagggtggt agtggaagcg gagctactaa cttcagcctg ctgaagcagg
     6181 ctggagacgt ggaggagaac cctggacctg cggccgcatg aaagggttcg atccctaccg
     6241 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     6301 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     6361 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6421 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     6481 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
     6541 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6781 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6841 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6901 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6961 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7021 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7081 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7141 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7201 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7261 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7321 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     7381 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     7441 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     7501 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     7561 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     7621 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     7681 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     7741 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     7801 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     7861 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     7921 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     7981 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     8041 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     8101 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     8161 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     8221 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8281 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8341 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8461 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8521 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8581 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8641 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8701 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8761 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8821 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8881 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9361 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9481 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9541 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9601 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9661 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9721 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9781 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9841 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9901 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9961 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10021 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10081 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10141 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10201 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10261 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10321 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10381 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10441 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10501 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10561 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10681 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    10741 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    10801 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    10861 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
    10921 gccagatata cgcgtt
//
