LOCUS       sequence-19756-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Seeler JS, Marchio A, Losson R, Desterro JM, Hay RT, Chambon P, 
            Dejean A
  TITLE     Common properties of nuclear body protein SP100 and TIF1alpha 
            chromatin factor: role of SUMO modification.
  JOURNAL   Mol Cell Biol. 2001 May . 21(10):3314-24.
  PUBMED    11313457
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2001 May . 21(10):3314-24."
COMMENT     Sequence Label: 6598-HisSUMO1GG
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    345..466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(382..401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     436..455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(500..517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(751..770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagaggatcg catcaccatc accatcacgg atctgaccag gaggcaaaac cttcaactga
       61 ggacttgggg gataagaagg aaggtgaata tattaaactc aaagtcattg gacaggatag
      121 cagtgagatt cacttcaaag tgaaaatgac aacacatctc aagaaactca aagaatcata
      181 ctgtcaaaga cagggtgttc caatgaattc actcaggttt ctctttgagg gtcagagaat
      241 tgctgataat catactccaa aagaactggg aatggaggaa gaagatgtga ttgaagttta
      301 tcaggaacaa acggggggtt aggatccaga tcttattaaa gcagaacttg tttattgcag
      361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga
      481 ctctagactc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc g
//
