LOCUS       sequence-197561-       11071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; 
            pCDNA3 vector backbone, mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   O'Geen H, Ren C, Nicolet CM, Perez AA, Halmai J, Le VM, Mackay JP, 
            Farnham PJ, Segal DJ
  TITLE     dCas9-based epigenome editing suggests acquisition of histone 
            methylation is not sufficient for target gene repression.
  JOURNAL   Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 
            10.1093/nar/gkx578.
  PUBMED    28973434
REFERENCE   2  (bases 1 to 11071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-09-29- 29"; volume: "45"; issue: 
            "17"; pages: "9901-9916"
COMMENT     Sequence Label: G9A[SET]-dCas9
FEATURES             Location/Qualifiers
     source          1..11071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             702..722
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             741..806
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(2043..2069,2070..2072,2073..4556,4557..4562,4563..4628,
                     4629..4631,4632..6146)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2043..2069,2070..2072,2073..4559,4560..4562,
                     4563..6146)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2043..6146
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6222..6269
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6288..6344
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6408..6427)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6452..6500
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6702..7130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6789..6808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6999..7020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7139..7159)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7144..7473
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7324..7459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7386..7405
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7540..8334
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7594..7613)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8204..8223
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8510..8631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8547..8566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8601..8620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8680..8696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8680..8696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8693..8715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8704..8720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8728..8734,8735..8752,8753..8758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8773..8794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8911..8928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9082..9670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9162..9181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9841..10632,10633..10701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10464..10483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10702..10806)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10881..10900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatcgaaccc ttgccaccat gcccaagaag aagaggaagg
      721 tgggaggctc cggaggaagc gactataagg accacgacgg agactacaag gatcatgata
      781 ttgattacaa agacgatgac gataagggtg gcgggtccgg cggtggatcc ggtaccggca
      841 gcgccgccat cgccgaagtc cttctgaatg cgcgctgtga cctccatgct gtcaactacc
      901 atggggacac ccccctgcac atcgcagctc gggagagcta ccatgactgc gtgctgttat
      961 tcctgtcacg tggggccaac cctgagctgc ggaacaaaga gggggacaca gcatgggacc
     1021 tgactcccga gcgctccgac gtgtggtttg cgcttcaact caaccgcaag ctccgacttg
     1081 gggtgggaaa tcgggccatc cgcacagaga agatcatctg ccgggacgtg gctcggggct
     1141 atgagaacgt gcccattccc tgtgtcaacg gtgtggatgg ggagccctgc cctgaggatt
     1201 acaagtacat ctcagagaac tgcgagacgt ccaccatgaa catcgatcgc aacatcaccc
     1261 acctgcagca ctgcacgtgt gtggacgact gctctagctc caactgcctg tgcggccagc
     1321 tcagcatccg gtgctggtat gacaaggatg ggcgattgct ccaggaattt aacaagattg
     1381 agcctccgct gattttcgag tgtaaccagg cgtgctcatg ctggagaaac tgcaagaacc
     1441 gggtcgtaca gagtggcatc aaggtgcggc tacagctcta ccgaacagcc aagatgggct
     1501 ggggggtccg cgccctgcag accatcccac aggggacctt catctgcgag tatgtcgggg
     1561 agctgatctc tgatgctgag gctgatgtga gagaggatga ttcttacctc ttcgacttag
     1621 acaacaagga tggagaggtg tactgcatag atgcccgtta ctatggcaac atcagccgct
     1681 tcatcaacca cctgtgtgac cccaacatca ttcccgtccg ggtcttcatg ctgcaccaag
     1741 acctgcgatt tccacgcatc gccttcttca gttcccgaga catccggact ggggaggagc
     1801 tagggtttga ctatggcgac cgcttctggg acatcaaaag caaatatttc acctgccaat
     1861 gtggctctga gaagtgcaag cactcagccg aagccattgc cctggagcag agccgtctgg
     1921 cccgcctgga cccacaccct gagctgctgc ccgagctcgg ctccctgccc cctgtcaaca
     1981 caagtaccgg agggagtggt ggaagcggcg gttctggtgg ctcaggcggc agcggccggc
     2041 caatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg
     2101 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
     2161 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
     2221 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
     2281 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
     2341 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
     2401 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
     2461 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
     2521 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
     2581 atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
     2641 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
     2701 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
     2761 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
     2821 aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
     2881 cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca
     2941 ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta
     3001 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca
     3061 gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg
     3121 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
     3181 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
     3241 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
     3301 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
     3361 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
     3421 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
     3481 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
     3541 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
     3601 tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
     3661 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
     3721 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
     3781 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
     3841 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
     3901 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
     3961 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
     4021 ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc
     4081 tggattttct taagtccgat ggatttgcca acaggaactt catgcagttg atccatgatg
     4141 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     4201 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     4261 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     4321 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     4381 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     4441 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     4501 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatg
     4561 ctatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     4621 ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     4681 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     4741 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     4801 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     4861 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     4921 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     4981 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     5041 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     5101 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     5161 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     5221 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     5281 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     5341 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     5401 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     5461 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     5521 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     5581 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     5641 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     5701 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     5761 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     5821 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     5881 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     5941 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     6001 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     6061 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     6121 tcgacctctc tcagctcggt ggagacggtg gtagtggagg ttcaggagga tccgggggga
     6181 gcggagggag cgctagcggt ggaggctccg gaggtggatc taaaaggccg gcggccacga
     6241 aaaaggccgg tcaggcaaaa aagaaaaagg gtggtagtgg aagcggagct actaacttca
     6301 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctgcggcc gcatgaaagg
     6361 gttcgatccc taccggttag taatgagttt aaacggggga ggctaactga aacacggaag
     6421 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     6481 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     6541 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     6601 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     6661 catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     6721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6901 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7021 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7081 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     7141 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7201 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7261 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7321 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7381 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     7441 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     7501 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     7561 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     7621 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     7681 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     7741 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     7801 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     7861 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7921 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7981 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     8041 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     8101 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8161 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8221 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     8281 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     8341 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     8401 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8461 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     8521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8641 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10801 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    10861 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    10921 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    10981 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    11041 cttcgcgatg tacgggccag atatacgcgt t
//
