LOCUS       sequence-197564-        6295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  split YFP. Plant expression of CBL10-YFPc-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6295)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM-35s-CBL10-YFPc-1
FEATURES             Location/Qualifiers
     source          1..6295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      138..726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     627..646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1038..1062
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1156..1408)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1428..1979)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2021..2200)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2669..3013
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2957..2977
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     3015..3031
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3016..3032
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3048..3072
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3814..3838)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3849..4100
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     4037..4058
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4392..4416
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4528..5237
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5310..5401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5402..5470,5471..6262)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5620..5639)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
       61 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      781 cacatggact ctagctagag gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
      841 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
      901 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
      961 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     1021 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     1081 cttaataaca cattgcggac gtttttaatg atcgaatact aacgtctcta ccagatatca
     1141 gcttgcatgc cggtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
     1201 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
     1261 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
     1321 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
     1381 aactttattg ccaaatgttt gaacgatctg cttgactcta ggggtcatca gatttcggtg
     1441 acgggcagga ccggacgggg cggcaccggc aggctgaagt ccagctgcca gaaacccacg
     1501 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
     1561 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
     1621 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
     1681 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
     1741 cgtgccttcc agggacccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg
     1801 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
     1861 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
     1921 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg
     1981 gtagatcccc tcgatcgagt tgagagtgaa tatgagactc taattggata ccgaggggaa
     2041 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
     2101 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     2161 agaaacccgc ggctcagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     2221 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     2281 atcagattgt cgtttcccgc cttcggtttg ggcgcgcccg gttcagaaga ccagagggct
     2341 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     2401 atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa atgccatcat
     2461 tgcgataaag gaaaggctat cgttcaagat gcctctaccg acagtggtcc caaagatgga
     2521 cccccaccca cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     2581 gtggattgat gtgatacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
     2641 atacagtctc agaagaccag agggctattg agacttttca acaaagggta atatcgggaa
     2701 acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg
     2761 aagatggctt ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
     2821 ctaccgacag tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag
     2881 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
     2941 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     3001 atttggagag gacctcgagg tcgacggtat cgataagctt cccatcaaca agtttgtaca
     3061 aaaaagcagg cttgatggaa caagtttcct ctagatcaag ctctctcact gtcggagagc
     3121 aattctgcgc cgtctttata cctttctttg ccattatcga tgtcctagtt tcctccgtcg
     3181 gacaatgctt cgattgccgc agcacatcgc ctcggacatg tcagcacgcg gatctggaac
     3241 gtcttgctcg tgaatctcaa ttttcagtca atgaagttga agctttgtat gagctattca
     3301 agaaattgag ctgttcaatt attgatgacg gcttgattca caaggaagag cttagactgg
     3361 cgctgttcca agctccatat ggtgagaacc tctttcttga tagggttttt gatttgttcg
     3421 atgaaaagaa gaatggggtt attgagtttg aggaattcat ccatgcactc agtgtctttc
     3481 atccctatgc atcaatccag gagaagaccg attttgcatt taggctctat gatctaagac
     3541 aaactggatt cattgagcga gaagaggtgc aacaaatggt ttctgctatt ctcttggaat
     3601 ctgatatgat gctatccgat gaacttctga ccatgattat tgataaaaca tttgctgatg
     3661 cagattctga caaagacggt aaaattagta aggatgaatg gaatgtctat gtgcataaac
     3721 atcctagctt gttgaagaat atgactcttc cttacctaaa ggatgtgacg acagcattcc
     3781 caagttttat attcaacact gaggttgaag actacccagc tttcttgtac aaagtggttg
     3841 atgggatgga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     3901 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3961 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     4021 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4081 gcatggacga gctgtacaag taatctagag tccgcaaaaa tcaccagtct ctctctacaa
     4141 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt
     4201 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     4261 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac
     4321 cgggcggccg ccaccgcggt ggagggggat cagattgtcg tttcccgcct tcagtttaaa
     4381 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     4441 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgta
     4501 catcaccgac gagcaaggca agaccgagcg cctttccgac gctcaccggg ctggttgccc
     4561 tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc
     4621 cgtgtgcgag acaccggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     4681 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     4741 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     4801 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     4861 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     4921 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     4981 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     5041 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     5101 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     5161 gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     5221 caccccaaaa atggcagcgc tggcagtcct tgccattgcc gggatcgggg cagtaacggg
     5281 atgggcgatc agcccgaaag ctacccctat ttgtttattt ttctaaatac attcaaatat
     5341 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5401 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5461 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5521 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5581 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5641 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5701 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5761 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5821 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5881 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5941 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6001 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6061 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6121 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6181 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6241 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttag
//
