LOCUS       sequence-197566-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A
  TITLE     An N-end rule pathway that recognizes proline and destroys 
            gluconeogenic enzymes.
  JOURNAL   Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 
            10.1126/science.aal3655.
  PUBMED    28126757
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-01-27"; volume: "355(6323). pii: 355/6323/eaal3655. 
            doi"; pages: " 10.1126/science.aal3655"
COMMENT     Sequence Label: pCSJ125
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..697
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     567..590
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ggcgattgga gctccaccgc ggcgacttta tcattatcaa tactgccatt tcaaagaata
       61 cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat tagcctttta
      121 attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa tatataacat
      181 cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg gagcccgctt
      241 tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca
      301 accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg cacaaacagg
      361 caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa tgatgacaca
      421 aggcaattga cccacgcatg tatctatctc attttcttac accttctatt accttctgct
      481 ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga aattattccc
      541 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
      601 tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa
      661 gaacttagtt tcgaataaac acacataaac aaacaaaatc gtacgggcct aaaacatacc
      721 agatcgccac ccgcgcttta atctggagag gtgaagaata cgaccaccta ggccttaaga
      781 accggtaaaa cataccagat cgccacccgc gctttaatct ggagaggtga agaatacgac
      841 cacctaccgg tcaacaacaa caacaacaac aacaacaact cgaggcctaa aacataccag
      901 atcgccaccc gcgctttaat ctggagaggt gaagaatacg accnnnnggc ntcgagaann
      961 nagnnnnnnc atgccncant c
//
