LOCUS       sequence-197567-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both 
            are contolled by tetracycline mediated TDH promter, test protein 
            expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A
  TITLE     An N-end rule pathway that recognizes proline and destroys 
            gluconeogenic enzymes.
  JOURNAL   Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 
            10.1126/science.aal3655.
  PUBMED    28126757
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2017-01-27"; volume: "355(6323). pii: 355/6323/eaal3655. 
            doi"; pages: " 10.1126/science.aal3655"
COMMENT     Sequence Label: pCSJ125
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..59
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     44..60
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(95..342)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     308..326
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(352..378)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(385..945)
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             complement(955..969)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tnaccctcac taaagggaca aaagctggta ccgggccccc cctcgaggtc gacggtatcg
       61 ataagcttga tatcgaattc ctgcagcccg ggccgcaaat taaagccttc gagcgtccca
      121 aaaccttctc aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa
      181 aaaaaagaaa aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat
      241 aaatagggac ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg
      301 ggggagggcg tgaatgtaag cgtgacataa ctaattacat gactcgagtt aggcgtaatc
      361 tgggacatcg tatgggtagg taccgtcttt cttctcgtag acttcaaact tatacttgat
      421 gcctttttcc tcctggacct cagagaggac gcctgggtat tctgggagaa gtttatattt
      481 ccccaaatca atttctggga aaaacgtgtc actttcaaat tcctgcatga tccttgtcac
      541 aaagagtctg aggtggcctg gttgattcat ggcttcctgg taaacagaac tgcctccgac
      601 tatccaaacc atgtctactt tacttgccaa ttccggttgt tcaataagtc ttaaggcatc
      661 atccaaactt ttggcaagaa aatgagcgcc tcgtggtggt tctttgagtt ctctactgag
      721 aactatatta attctgtcct ttaaaggtcg attcttctca ggaatggaga accaggtttt
      781 cctacccata atcaccagat tctgtttacc ttccactgaa gaggttgtgg tcattctttg
      841 gaagtacttg aactcgttcc tgagcggagg ccagggtagg tctccgttct tgccaatccc
      901 catattttgg gacacggcga cgatgcagtt caatggtcga accatggacc cacccttgtc
      961 atcgtcgtcc tngtagtcca tgaantcga
//
