LOCUS       sequence-197579-        3624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ompR from a TetR reuglated promoter under inducible ATC 
            control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ
  TITLE     Phosphatase activity tunes two-component system sensor detection 
            threshold.
  JOURNAL   Nat Commun. 2018 Apr 12;9(1):1433. doi: 10.1038/s41467-018-03929-y.
  PUBMED    29650958
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-12"; volume: "9(1):1433. doi"; pages: " 
            10.1038/s41467-018-03929-y"
COMMENT     Sequence Label: pSR26-2
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(151..739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(231..250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(827..921)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(942..1601)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1516..1535
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1602..1704)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1770..1788
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1809..1827,1828..1833,1834..1850,1851..1856,
                     1857..1882)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1809..1827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1834..1852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2658..2729
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2745..2772
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2824..2858
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             2893..3519
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2969..2988)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      3525..3611
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctaggcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
       61 cacagaatca ggggataacg catgcaagaa catgtgagca aaaggccagc aaaaggccag
      121 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      181 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      241 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      301 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      361 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      421 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      481 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      541 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
      601 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      661 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      721 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      781 gaacgaaaac tcacgttaag ggattttggt catgactagt gcttggattc tcaccaataa
      841 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      901 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      961 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1021 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     1081 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     1141 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     1201 aaataggcca ggttttcacc ataacacgcc acatcttgcg aatatatgtg tagaaactgc
     1261 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     1321 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1381 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1441 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1501 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1561 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1621 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1681 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtctaagaaa
     1741 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     1801 acctcgagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     1861 acatcagcag gacgcactga ccgcataaag gacttagcaa agcccaattt taaacaaatg
     1921 caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa
     1981 cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga acagatggat
     2041 cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttaatgtt acctggtgaa
     2101 gatggcttgt cgatttgccg acgtcttcgt agtcagagca acccgatgcc gatcattatg
     2161 gtgacggcga aaggggaaga agtggaccgt atcgtaggcc tggagattgg cgctgacgac
     2221 tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtatccgtgc ggtgctgcgt
     2281 cgtcaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat tgctttcggt
     2341 aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagacgagcc gatgccgctc
     2401 accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga gccgctctcc
     2461 cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc
     2521 gacgtgcaga tttcgcgtct gcgccgcatg gtggaagaag atccagcgca tccgcgttac
     2581 attcagaccg tctggggtct gggctacgtc tttgtaccgg acggctctaa agcatgataa
     2641 taatctagac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2701 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2761 ggcctttctg cgtttatagg tacccgtgga tcctctagag tcgacctgca ggcatgcaag
     2821 ctttttacgg ctagctcagt cctaggtata gtgctagccc agccagagaa acactcttta
     2881 acagggggta gtatgatgtc tcgtttagat aaaagtaaag tgattaacag cgcattagag
     2941 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     3001 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     3061 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     3121 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     3181 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     3241 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     3301 gcagtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     3361 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     3421 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     3481 ggattagaaa aacaacttaa atgtgaaagt gggtcttaag gcatcaaata aaacgaaagg
     3541 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     3601 gtaggacaaa tccgccgccc taga
//
