LOCUS       sequence-197585-        4515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Conkright MD, Guzman E, Flechner L, Su AI, Hogenesch JB, Montminy M
  TITLE     Genome-wide analysis of CREB target genes reveals a core promoter 
            requirement for cAMP responsiveness.
  JOURNAL   Mol Cell. 2003 Apr . 11(4):1101-8.
  PUBMED    12718894
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2003 Apr . 11(4):1101-8."
COMMENT     Sequence Label: pCDNA GAL4 CREB 1-341 M1
FEATURES             Location/Qualifiers
     source          1..4515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(29..49)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             364..804
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2025..2159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2062..2081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2116..2135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2169..2188)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2170..2188)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2275..2292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2446..3034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2526..2545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3205..3996,3997..4065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3828..3847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4066..4170)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4238..4256
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4294..4316)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4416..4435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaggtga cactatagaa ctcgatgtgg aatgtgtgtc agttagggtg tggaaagtcc
       61 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaggaaa
      121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      181 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      301 tgagctattc cagaagtagt gaagaggctt ttttggagga gatctaagct agcctcctga
      361 aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact taaaaagctc
      421 aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc
      481 tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga agtggaatca
      541 aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga ccttgacatg
      601 attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt atttgtacaa
      661 gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac tgatatgcct
      721 ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag tagtaacaaa
      781 ggtcaaagac agttgactgt atcgccggaa ttcggatccg actctggagc agacaaccag
      841 cagagtggag atgctgctgt aacagaagct gaaagtcaac aaatgacagt tcaagcccag
      901 ccacagattg ccacattagc ccaggtatcc atgccagcag ctcatgcgac gtcatctgct
      961 cccactgtaa ccttagtgca gctgcccaat gggcagacag tccaggtcca tggggtcatc
     1021 caggcggccc agccatcagt tattcagtct ccacaagtcc aaacagttca gtcttcctgt
     1081 aaggacttaa aaagactttt ctccggaact cagatttcaa ctattgcaga aagtgaagat
     1141 tcacaggagt ctgtggatag tgtaactgat tcccaaaaac gaagggaaat cctttcaagg
     1201 aggcctgcct acaggaaaat tttgaatgac ttatcttctg atgcaccagg ggtgccaagg
     1261 attgaagaag aaaaatcaga agaagagact tcagcccctg ccatcaccac tgtaacagtg
     1321 ccaaccccga tttaccaaac tagcagtggg cagtatattg ccattaccca gggaggagca
     1381 atacagctgg ctaacaatgg taccgatggg gtacagggcc tgcagacatt aaccatgacc
     1441 aatgcagctg ccactcagcc gggtactacc attctacaat atgcacagac cactgatgga
     1501 cagcagattc tagtgcccag caaccaagtt gttgttcaag ctgcctctgg tgatgtacaa
     1561 acataccaga ttcgcacagc acccactagc accattgccc ctggagttgt tatggcgtcc
     1621 tccccagcac ttcctacaca gcctgctgaa gaagcagcac gaaagagaga ggttcgtcta
     1681 atgaagaaca gggaagcagc aagagaatgt cgtagaaaga agaaagaata tgtgaaatgt
     1741 ttagagaaca gagtggcagt gcttgaaaac caaaacaaaa cattgattga ggagctaaaa
     1801 gcacttaagg acctttactg ccacaagtca gattaatttg ggatttaaat tttcacctat
     1861 tgtggtggaa aatggactgg attgggggta ccgtcgagca tgcatctaga taagtaatga
     1921 tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc
     1981 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2041 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2101 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     2161 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg
     2221 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2281 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2341 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2401 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2461 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2521 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2581 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2641 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2701 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2761 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2821 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2881 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2941 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3001 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3061 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3121 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3181 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3241 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3301 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3361 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3421 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3481 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3541 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3601 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3661 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3721 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3781 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3841 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3901 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3961 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4021 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4081 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4141 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     4201 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     4261 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     4321 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     4381 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     4441 agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg
     4501 tatcatacac atacg
//
