LOCUS       sequence-197588-       10015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Takagi J, Petre BM, Walz T, Springer TA
  TITLE     Global conformational rearrangements in integrin extracellular 
            domains in outside-in and inside-out signaling.
  JOURNAL   Cell. 2002 Sep 6. 110(5):599-11.
  PUBMED    12230977
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2002 
            Sep 6. 110(5):599-11."
COMMENT     Sequence Label: pEF1-alpha-V
FEATURES             Location/Qualifiers
     source          1..10015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        155..1333
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          386..1324
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1281..1301
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1350..1369
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1350..1368
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5328..5369
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5379..5396
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5419..5436)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5425..5649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5695..6123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5782..5801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5992..6013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6132..6152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6137..6466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6317..6452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6379..6398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6533..7327
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6587..6606)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7197..7216
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7503..7624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7540..7559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7594..7613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7673..7689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7673..7689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7686..7708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7697..7713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7721..7727,7728..7745,7746..7751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7766..7787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7904..7921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8064..8652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8144..8163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8823..9614,9615..9683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9446..9465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9684..9788)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9863..9882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactaggct
       61 tttgcaaaaa gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
      121 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
      181 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      241 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      301 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      361 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      421 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      481 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      541 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      601 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      661 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
      721 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
      781 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
      841 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
      901 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
      961 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     1021 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     1081 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     1141 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     1201 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     1261 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     1321 tcaggtgtcg tgaggaatta gcttggtact aatacgactc actataggga gacccaagct
     1381 ggctaggtaa gcttggtacc gagctcggat ccactagagg cctccgcggc cgcgaattca
     1441 tctgtcgact gctacccgca ggcggcgggc cgcgggcact gggcgcctcg ctggggcggg
     1501 gggaggtggc taccgctccc ggcttggcgt cccgcgcgca cttcggcgat ggcttttccg
     1561 ccgcggcgac ggctgcgcct cggtccccgc ggcctcccgc ttcttctctc gggactcctg
     1621 ctacctctgt gccgcgcctt caacctagac gtggacagtc ctgccgagta ctctggcccc
     1681 gagggaagtt acttcggctt cgccgtggat ttcttcgtgc ccagcgcgtc ttcccggatg
     1741 tttcttctcg tgggagctcc caaagcaaac accacccagc ctgggattgt ggaaggaggg
     1801 caggtcctca aatgtgactg gtcttctacc cgccggtgcc agccaattga atttgatgca
     1861 acaggcaata gagattatgc caaggatgat ccattggaat ttaagtccca tcagtggttt
     1921 ggagcatctg tgaggtcgaa acaggataaa attttggcct gtgccccatt gtaccattgg
     1981 agaactgaga tgaaacagga gcgagagcct gttggaacat gctttcttca agatggaaca
     2041 aagactgttg agtatgctcc atgtagatca caagatattg atgctgatgg acagggattt
     2101 tgtcaaggag gattcagcat tgattttact aaagctgaca gagtacttct tggtggtcct
     2161 ggtagctttt attggcaagg tcagcttatt tcggatcaag tggcagaaat cgtatctaaa
     2221 tacgacccca atgtttacag catcaagtat aataaccaat tagcaactcg gactgcacaa
     2281 gctatttttg atgacagcta tttgggttat tctgtggctg tcggagattt caatggtgat
     2341 ggcatagatg actttgtttc aggagttcca agagcagcaa ggactttggg aatggtttat
     2401 atttatgatg ggaagaacat gtcctcctta tacaatttta ctggcgagca gatggctgca
     2461 tatttcggat tttctgtagc tgccactgac attaatggag atgattatgc agatgtgttt
     2521 attggagcac ctctcttcat ggatcgtggc tctgatggca aactccaaga ggtggggcag
     2581 gtctcagtgt ctctacagag agcttcagga gacttccaga cgacaaagct gaatggattt
     2641 gaggtctttg cacggtttgg cagtgccata gctcctttgg gagatctgga ccaggatggt
     2701 ttcaatgata ttgcaattgc tgctccatat gggggtgaag ataaaaaagg aattgtttat
     2761 atcttcaatg gaagatcaac aggcttgaac gcagtcccat ctcaaatcct tgaagggcag
     2821 tgggctgctc gaagcatgcc accaagcttt ggctattcaa tgaaaggagc cacagatata
     2881 gacaaaaatg gatatccaga cttaattgta ggagcttttg gtgtagatcg agctatctta
     2941 tacagggcca gaccagttat cactgtaaat gctggtcttg aagtgtaccc tagcatttta
     3001 aatcaagaca ataaaacctg ctcactgcct ggaacagctc tcaaagtttc ctgttttaat
     3061 gttaggttct gcttaaaggc agatggcaaa ggagtacttc ccaggaaact taatttccag
     3121 gtggaacttc ttttggataa actcaagcaa aagggagcaa ttcgacgagc actgtttctc
     3181 tacagcaggt ccccaagtca ctccaagaac atgactattt caaggggggg actgatgcag
     3241 tgtgaggaat tgatagcgta tctgcgggat gaatctgaat ttagagacaa actcactcca
     3301 attactattt ttatggaata tcggttggat tatagaacag ctgctgatac aacaggcttg
     3361 caacccattc ttaaccagtt cacgcctgct aacattagtc gacaggctca cattctactt
     3421 gactgtggtg aagacaatgt ctgtaaaccc aagctggaag tttctgtaga tagtgatcaa
     3481 aagaagatct atattgggga tgacaaccct ctgacattga ttgttaaggc tcagaatcaa
     3541 ggagaaggtg cctacgaagc tgagctcatc gtttccattc cactgcaggc tgatttcatc
     3601 ggggttgtcc gaaacaatga agccttagca agactttcct gtgcatttaa gacagaaaac
     3661 caaactcgcc aggtggtatg tgaccttgga aacccaatga aggctggaac tcaactctta
     3721 gctggtcttc gtttcagtgt gcaccagcag tcagagatgg atacttctgt gaaatttgac
     3781 ttacaaatcc aaagctcaaa tctatttgac aaagtaagcc cagttgtatc tcacaaagtt
     3841 gatcttgctg ttttagctgc agttgagata agaggagtct cgagtcctga tcatatcttt
     3901 cttccgattc caaactggga gcacaaggag aaccctgaga ctgaagaaga tgttgggcca
     3961 gttgttcagc acatctatga gctgagaaac aatggtccaa gttcattcag caaggcaatg
     4021 ctccatcttc agtggcctta caaatataat aataacactc tgttgtatat ccttcattat
     4081 gatattgatg gaccaatgaa ctgcacttca gatatggaga tcaacccttt gagaattaag
     4141 atctcatctt tgcaaacaac tgaaaagaat gacacggttg ccgggcaagg tgagcgggac
     4201 catctcatca ctaagcggga tcttgccctc agtgaaggag atattcacac tttgggttgt
     4261 ggagttgctc agtgcttgaa gattgtctgc caagttggga gattagacag aggaaagagt
     4321 gcaatcttgt acgtaaagtc attactgtgg actgagactt ttatgaataa agaaaatcag
     4381 aatcattcct attctctgaa gtcgtctgct tcatttaatg tcatagagtt tccttataag
     4441 aatcttccaa ttgaggatat caccaactcc acattggtta ccactaatgt cacctggggc
     4501 attcagccag cgcccatgcc tgtgcctgtg tgggtgatca ttttagcagt tctagcagga
     4561 ttgttgctac tggctgtttt ggtatttgta atgtacagga tgggcttttt taaacgggtc
     4621 cggccacctc aagaagaaca agaaagggag cagcttcaac ctcatgaaaa tggtgaagga
     4681 aactcagaaa cttaactgca gtttttaagt tatgctacat cttgacccac tagaattagc
     4741 aactttatta tagatttaaa ctttcttcat gaggagtaaa aatccaaggc tttactgctg
     4801 atagtgctaa ttggcattaa ccacaaaatg agaattatat ttgtcaacct tctccttata
     4861 aataagttca gacatacatt taataacata gggtgacttg tgtttttagg tatttaaata
     4921 ataaaatttc aagggatagt ttttattcaa tgtatataag acaggtagtg cctgatttac
     4981 tactttatat aaaatagtac ctccttcagt tactgtttct gatttaatgt acggaacttt
     5041 atttgttgtt gttgttgttg ttgttgttgt tgttttaaag cagtccaaat ttggacctta
     5101 gcaatcatgt cttttgtata ggtacttaat gttaatacat attacactac agtttacttt
     5161 tcagaatact aaagacttta taactgcatg aacttggatt tttttaatca ctcatatggt
     5221 agaattttat aaacacatac atgataccat ccaaattctt gcttttaata acaaaggtac
     5281 aatatccagc acagtggcgg ccgctcgagt ctagagggcc cttcgaaggt aagcctatcc
     5341 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     5401 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5461 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5521 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5581 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5641 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6121 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6481 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6541 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6601 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6661 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6721 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6781 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6841 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6901 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6961 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     7021 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     7081 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7141 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7201 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7261 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7321 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat
     7381 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7441 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7501 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7561 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7621 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7741 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7801 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc caggaaccgt
     8041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8701 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8761 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8821 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8881 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8941 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9001 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9061 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9121 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9181 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9241 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9301 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9361 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9421 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9481 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9541 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9601 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9661 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9721 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9781 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9841 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9901 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     9961 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttt
//
