LOCUS       sequence-197594-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter - constitutive expression of mOrange2 
            (549/565) localized in the nucleus, EGFP (488/507) untargeted and 
            visualized in the cytoplasm, and Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Calderon GA, Thai P, Hsu CW, Grigoryan B, Gibson SM, Dickinson ME, 
            Miller JS
  TITLE     Tubulogenesis of co-cultured human iPS-derived endothelial cells and
            human mesenchymal stem cells in fibrin and gelatin methacrylate 
            gels.
  JOURNAL   Biomater Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h.
  PUBMED    28661522
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biomater 
            Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h."
COMMENT     Sequence Label: hEF1-alpha-H2B-mOrange2-IRES-EGFP PGK-Puromycin
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    148..381
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             566..610
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    877..994
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    1039..1087
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1112..1131
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1189..1788)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1272..1292)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1769..1788
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1800..2299)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1848..1867)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2241..2264
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        2342..3511
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2580..3502
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3459..3479
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4707..5258
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4851..4868)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5078..5097
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5276..5278,5279..5281,5282..5992)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    6843..7431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6896..6916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7314..7325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7524..7757
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        7794..7885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7886..8695
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7953..7972)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8781..9369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9270..9289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9507..9733
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9734..9914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9961..10086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
       61 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
      121 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
      181 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
      241 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
      301 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
      361 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
      421 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
      481 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
      541 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
      601 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
      661 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
      721 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
      781 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
      841 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
      901 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      961 attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta gcctcgagat
     1021 cccgaacccc gcgtttatga acaaacgacc caacacccgt gcgttttatt ctgtcttttt
     1081 attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc
     1141 ccccatctcc cggtcgagaa gcttacgcgt gaatttcgac gcggccgctc aggcaccggg
     1201 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     1261 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gatgtctcca ggaaggcggg
     1321 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     1381 gccctggtgg tcgggcgaca cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     1441 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     1501 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     1561 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     1621 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     1681 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     1741 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcattg tggcggcgac
     1801 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
     1861 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
     1921 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
     1981 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
     2041 tgcacgagac tagtgagtcg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
     2101 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
     2161 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca
     2221 cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg
     2281 aaaagcgcct cccctacccg gtagagaaac ttgatctgtc gccgcaattc aagcttcgtg
     2341 aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2401 gggaggctct ggctgcaggt aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2461 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2521 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2581 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2641 cttgaattac ttccacctgg ctccagtacg tgattcttga tcccgagctg gagccagggg
     2701 cgggccttgc gctttaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     2761 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     2821 gtctctagcc atttaaaatt tttgatgacg tgctgcgacg ctttttttct ggcaagatag
     2881 tcttgtaaat gcgggccagg atctgcacac tggtatttcg gtttttgggc ccgcggccgg
     2941 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3001 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3061 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3121 agcggaaaga tggccgcttc ccggccctgc tccagggggc tcaaaatgga ggacgcggcg
     3181 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3241 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctg
     3301 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     3361 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     3421 ggaatttggc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     3481 aagttttttt cttccatttc aggtgtcgtg aacacctgcg aacggggtga gacgacaccc
     3541 aagctgtcta gagccgccac catgccagaa cccgccaagt ccgctcctgc tccgaagaag
     3601 ggctcgaaga aagcagtgac gaaggcccag aagaagggcg ggaagaagcg caagagatcc
     3661 cggaaggaat cgtactccat ctacgtgtac aaggtcctga aacaagtgca ccccgacacc
     3721 ggcatcagct cgaaggccat gggaatcatg aacagctttg tgaacgacat tttcgaacgg
     3781 atcgcggggg aggccagcag gctggcccac tacaacaagc ggtcaaccat cacgtctagg
     3841 gagattcaaa ccgccgtccg actgttgctg cccggagaac tggccaagca cgcggtgtca
     3901 gagggtacca aggccgtgac taagtacacc agcgccaaag accccgtgtg cgaacatcca
     3961 ggcatttgcg ggtcgactat ggtgtcaaag ggagaggaaa acaacatggc gatcattaag
     4021 gagttcatgc gcttcaaggt ccgcatggag ggctccgtga acggacacga gttcgaaatc
     4081 gagggagagg gagaaggacg gccgtacgaa ggattccaga ctgccaaact caaggtcacc
     4141 aaggggggtc ccctgccctt cgcatgggac attctgtccc ctcacttcac ctacggctcc
     4201 aaggcctacg tcaagcaccc agccgacatc cctgactact tcaagctgag cttcccggaa
     4261 ggcttcaaat gggaaagagt gatgaattac gaagatggtg gagtggtcac tgtgacccag
     4321 gactcatccc tgcaagatgg ggagttcatc tacaaagtca agctcagggg tacaaatttc
     4381 ccgtccgacg gacccgtgat gcagaagaaa accatgggat gggaggcttc gtccgaacgc
     4441 atgtatccgg aggacggcgc cctgaagggg aagatcaaga tgcgcctgaa gctcaaggat
     4501 ggaggccact acacctccga ggtcaagact acttacaagg ccaagaagcc ggtgcagctg
     4561 cctggagcgt acattgtgga tatcaagctt gacatcacct cccataacga agattacacc
     4621 atcgtggaac agtatgagcg ggcggaagga cgccactcga ccgggggcat ggacgagctc
     4681 tacaaatgag aattcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4741 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4801 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4861 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4921 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4981 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5041 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5101 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5161 gtacacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     5221 ggggacgtgg ttttcctttg aaaaacacga tgataatatg ctagcggcca caaccatggt
     5281 gtcgaaggga gaagaattgt ttactggagt cgtgcccatc ctggtggaat tggacggaga
     5341 tgtgaacggt cacaagttct ccgtgtcggg agaaggggag ggcgacgcga cctacggaaa
     5401 gcttacactc aagttcatct gcactactgg gaagctccca gtgccgtggc ccactctcgt
     5461 gaccaccctg acctacggcg tgcaatgctt ttcccggtac cctgatcaca tgaagcagca
     5521 cgacttcttc aagagcgcca tgccggaagg ttacgtgcag gagcggacca tctttttcaa
     5581 ggacgacggg aactacaaga ctcgggccga agtgaagttc gagggggaca ccctcgtgaa
     5641 cagaattgag ctgaagggaa tcgacttcaa agaagatgga aacatcctgg gccataaact
     5701 ggagtacaac tacaattcac acaacgtcta cattatggcg gataagcaga agaatgggat
     5761 caaggtcaac ttcaagatta gacataacat cgaggacggt tccgtgcagc tggccgacca
     5821 ctaccagcaa aacaccccta tcggcgacgg cccggtgctc ctgcctgata accactacct
     5881 gagcacccag tccgctctgt ccaaggaccc gaacgagaag cgcgaccata tggtgctgct
     5941 tgagttcgtc accgccgccg gaattaccct cggcatggat gaactgtaca aatagggatc
     6001 ccgtctctaa aactgttctc atcacatcat atcaaggtta tataccatca atattgccac
     6061 agatgttact tagcctttta atatttctct aatttagtgt atatgcaatg atagttctct
     6121 gatttctgag attgagtttc tcatgtgtaa tgattattta gagtttctct ttcatctgtt
     6181 caaatttttg tctagtttta ttttttactg atttgtaaga cttcttttta taatctgcat
     6241 attacaattc tctttactgg ggtgttgcaa atattttctg tcattctatg gcctgacttt
     6301 tcttaatggt tttttaattt taaaaataag tcttaatatt catgcaatct aattaacaat
     6361 cttttctttg tggttaggac tttgagtcat aagaaatttt tctctacact gaagtcatga
     6421 tggcatgctt ctatattatt ttctaaaaga tttaaagttt tgccttctcc atttagactt
     6481 ataattcact ggaatttttt tgtgtgtatg gtatgacata tgggttccct tttatttttt
     6541 acatataaat atatttccct gtttttctaa aaaagaaaaa gatcatcatt ttcccattgt
     6601 aaaatgccat atttttttca taggtcactt acatatatca atgggtctgt ttctgagctc
     6661 tactctattt tatcagcctc actgtctatc cccacacatc tcatgctttg ctctaaatct
     6721 tgatatttag tggaacattc tttcccattt tgttctacaa gaatattttt gttattgtct
     6781 ttgggctttc tatatacatt ttgaaatgag gttgacaagt tagcgtgacg tcaattcccg
     6841 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7201 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7261 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcctc
     7381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggaat
     7441 tgcgcacgca gtggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7501 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7561 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7621 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7681 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7741 gtggaaaatc tctagcatca gaattggtta attggttgta acactgaccc ctatttgttt
     7801 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7861 tcaataatat tgaaaaagga agaatatgag ccatattcaa cgggaaacgt cgaggccgcg
     7921 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     7981 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     8041 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     8101 gctgacggaa tttatgccac ttccgaccat caagcatttt atccgtactc ctgatgatgc
     8161 atggttactc accactgcga tccccggaaa aacagcgttc caggtattag aagaatatcc
     8221 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcactcgat
     8281 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc aggcgcaatc
     8341 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     8401 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     8461 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     8521 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     8581 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     8641 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaactcat
     8701 gaccaaaatc ccttaacgtg agttacgcgc gcgtcgttcc actgagcgtc agaccccgta
     8761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8941 ccgtagttag cccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9421 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9481 gagtgagctg ataccgctca gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9541 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9601 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9661 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9721 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9781 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9901 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9961 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10021 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10081 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10141 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10201 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10261 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10321 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10381 atagaggaac agcaaaacaa aagtaagacc accgcacagc aagcggccgc t
//
