LOCUS       sequence-197632-       12786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-intron-BaLgp160
FEATURES             Location/Qualifiers
     source          1..12786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        248..393
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             436..1473
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1471..1490)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1515..1563
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2217..2596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2597..2800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2751..2771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2797..2821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2898..3030
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3013..3032
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3075..3093
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5046..5090
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    5680..5814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5704..5723)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5758..5777
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5957..5979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6480..8269
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(8571..10496)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGXGGXGGAGXGGXGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10931..10949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11017..11121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11122..11190,11191..11982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11340..11359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12153..12741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12642..12661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
       61 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      121 cgcgaacgcc agcaagacgt agccctccgt tagcgaggtg ccgccctgct tcatccccgt
      181 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
      241 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat
      301 gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
      361 gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatccc
      421 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
      481 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
      541 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
      601 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
      661 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
      721 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
      781 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
      841 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
      901 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
      961 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     1021 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     1081 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     1141 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     1201 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     1261 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     1321 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     1381 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     1441 cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     1501 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     1561 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     1621 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     1681 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg
     1741 tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt
     1801 gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga
     1861 tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc
     1921 aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc
     1981 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
     2041 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
     2101 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     2161 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
     2221 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2401 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2461 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2521 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
     2581 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
     2641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2701 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
     2761 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     2821 ctctagaagc tgggtaccct tctgacacaa cagtctcgaa cttaagctgc agaagttggt
     2881 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
     2941 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
     3001 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt
     3061 aaggctagag tacttaatac gactcactat aggctagcgc caccatgccc atggggagct
     3121 tgcaaccgct cgccactctc tatctcctcg gcatgttggt agcatctgtg ctggctgagg
     3181 aaaagctctg ggtgactgtg tattacgggg tcccagtttg gaaggaggct actaccacgc
     3241 tcttctgcgc ctccgaccgg aaggcatacg acaccgaggt gcataatgtg tgggccacac
     3301 acgcctgtgt gcctaccgac cccaacccac aggaagtgga actgaagaac gtgactgaga
     3361 atttcaatat gtggaaaaat aatatggtgg agcagatgca cgaggacatt attagcctct
     3421 gggatcagtc ccttaaaccc tgcgtgaaac tgacaccgct gtgcgtaacg ctgaattgta
     3481 ctgacctccg gaatgccacg aacggaaacg acacaaatac cacgtcaagc tcccgaggga
     3541 tggtgggagg gggagaaatg aagaactgct cattcaatat aacgacgaat atccgaggga
     3601 aggttcagaa agagtatgcc ctgttttaca aactggacat cgctcctatc gacaacaaca
     3661 gcaataacag gtaccgcctg attagctgca acacttctgt gattacccag gcctgcccca
     3721 aggtgagctt cgagcctatc ccaatccact actgcgcccc agcaggcttt gccatcctga
     3781 aatgcaagga caagaagttc aacggcaaag gcccatgtac aaatgtcagt acagtacagt
     3841 gcacacacgg tatccgccca gttgtctcta cacaactgct gctgaatgga tccttggcag
     3901 aggaagaggt ggtgatcaga tctgctaact tcgccgataa tgctaaagtc atcattgttc
     3961 agctgaacga gtctgtcgaa atcaattgta ctcgcccaaa caacaacacg cgcaagagca
     4021 ttcatatcgg accgggcagg gccttttaca caacagggga gattattggt gatattagac
     4081 aagcccactg caacctcagt cgagcaaagt ggaatgatac tctcaacaaa attgttatca
     4141 aattgcggga acagtttggg aacaagacaa tcgtatttaa gcactcatcc ggaggcgatc
     4201 cagaaatcgt gacacactct tttaactgcg gtggagaatt tttctactgc aactccaccc
     4261 agctgttcaa cagtacgtgg aacgtgacag aggagtctaa caacaccgtg gagaataata
     4321 cgatcaccct tccatgtcgg attaagcaga tcatcaatat gtggcaggag gtgggaagag
     4381 caatgtacgc gccgcccatt aggggccaga tcagatgcag ttcaaatatc acaggcctgc
     4441 tcctgacacg cgacggaggc ccagaggaca acaagacaga ggtgtttagg cccggcggcg
     4501 gcgacatgcg agataattgg aggtctgaat tgtacaagta caaggtggtg aaaatcgaac
     4561 ccctgggagt ggcgcctact aaggcaaagc gccgcgttgt gcagagagag aagagggcag
     4621 tgggaattgg ggcggttttt cttgggttcc tgggggctgc aggtagcacc atgggagcgg
     4681 ccgctatgac actgacagtg caagccagac tcctgctgtc tggtattgtc caacagcaaa
     4741 acaatttgct ccgcgcaata gaagcccagc agcacctgct ccaactgact gtgtggggca
     4801 ttaaacaatt gcaggctcgc gtgctggcag tcgaacggta cctgagggat caacagctcc
     4861 tgggcatctg gggctgctcc gggaaactca tctgtacgac agcggtccct tggaatgcca
     4921 gctggtccaa caaaagcctc aacaagattt gggacaatat gacgtggatt gagtgggaca
     4981 gggaaattaa caattatacc tcaatcatat attctctgat tgaagagtcc cagaatcagc
     5041 aagagaagaa tgagcaggaa ttgttggaac ttgataaatg ggcaagcctg tggaactggt
     5101 ttgacataac caaatggttg tggtatatca aaatcttcat aatgatcgtc ggcggactca
     5161 tcgggctccg aattgtgttc tcagtcctca gcattgtgaa tagggtgcgc cagggctatt
     5221 ctcccctgag cttccagacc catctgccct ctagccgagg accagatagg cctggtggga
     5281 tcgaggaaga aggcggggaa cgggacaggg ataggagtgg tcctcttgtt aacgggttcc
     5341 tggcacttat atgggtggac ttgagaagcc tgttcctgtt ctcctatcat aggctccggg
     5401 atcttctcct gatagtcatg cggatcgtcg agctgttggg gcttgctggc ggctgggaag
     5461 tactgaaata ttggtggaat ctgttgcagt attggagcca ggaacttaag aactctgctg
     5521 tgagtctcct taatgccaca gctgttgctg tggccgaagg aaccgatcga gttattgaag
     5581 tactgcaacg agcagttcgc gctattctgc atatcccacg acgcattcgg caggggctgg
     5641 agagagctct cctgtagtaa ctcgaggccg gcaaggccgg atccagacat gataagatac
     5701 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5761 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     5821 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     5881 aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc gcgcgtttcg
     5941 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6001 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6061 ggggcgcagc catgaggtcg actctagacg gggcagtgca tgtaatccct tcagttggtt
     6121 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     6181 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     6241 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     6301 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     6361 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     6421 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     6481 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     6541 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     6601 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     6661 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     6721 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     6781 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     6841 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     6901 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     6961 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     7021 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     7081 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     7141 acaaggggaa agagagtgga cgccgacagc agcgcactcc actggttgtc tctaacaccc
     7201 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     7261 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     7321 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     7381 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     7441 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     7501 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     7561 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     7621 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     7681 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     7741 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     7801 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     7861 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     7921 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     7981 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8041 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8101 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     8161 gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg
     8221 ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc
     8281 cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca
     8341 ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt
     8401 gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga
     8461 tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg
     8521 gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc
     8581 ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc
     8641 gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag
     8701 ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta
     8761 atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag
     8821 acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg
     8881 agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg
     8941 ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg
     9001 tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc
     9061 tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg
     9121 cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct
     9181 gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct
     9241 tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga
     9301 gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc
     9361 ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt
     9421 tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct
     9481 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
     9541 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     9601 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct
     9661 cctgcccctc ctgcccctcc tcctgctcct gcccctccts cccctcctsc tcctgcccct
     9721 cctscccctc ctsctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc
     9781 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     9841 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     9901 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     9961 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
    10021 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
    10081 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
    10141 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
    10201 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
    10261 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
    10321 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
    10381 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
    10441 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
    10501 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
    10561 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
    10621 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
    10681 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
    10741 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
    10801 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
    10861 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
    10921 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    10981 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    11041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    11101 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    11161 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    11221 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    11281 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    11341 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
    11401 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    11461 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    11521 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    11581 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    11641 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
    11701 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    11761 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    11821 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    11881 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11941 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    12001 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    12061 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    12121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    12181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    12241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    12301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    12361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    12421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    12481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    12541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    12601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    12661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    12721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    12781 tgctgc
//
