LOCUS       sequence-197641-       12362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; QES mutant with enhanced binding to antibody PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842gp160-QES.04
FEATURES             Location/Qualifiers
     source          1..12362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..229
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             272..1309
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1307..1326)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1351..1399
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2053..2432
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2433..2636
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2587..2607
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2633..2657
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5257..5391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5281..5300)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5335..5354
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5534..5556)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6057..7846
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(8148..10073)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGGGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10508..10526)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10594..10698
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10699..10767,10768..11559)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10917..10936)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11730..12318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12219..12238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
       61 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
      121 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
      181 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
      241 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
      301 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
      361 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
      421 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
      481 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
      541 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
      601 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
      661 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
      721 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
      781 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
      841 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
      901 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
      961 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1021 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     1081 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1141 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1201 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     1261 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
     1321 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     1381 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     1441 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     1501 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     1561 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
     1621 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
     1681 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
     1741 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
     1801 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     1861 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     1921 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     1981 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     2041 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2101 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2161 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2221 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2281 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
     2341 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
     2401 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
     2461 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2521 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
     2581 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2641 tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc atgccaatgg
     2701 gatcactgca gcccttggca acgctgtact tgcttggtat gcttgtggca tctgttcttg
     2761 ctgtcgataa tttgtgggtc acagtgtact acggagtacc tgtatggaaa gaggcggaaa
     2821 ctactctttt ttgcgcgtca gacgcaaagg catatgagac tgaaaagcat aatgtatggg
     2881 ctacccatgc gtgcgtgccc acagacccca acccccaaga aatccatctg gagaacgtta
     2941 ctgaagaatt taatatgtgg aagaataata tggtggaaca gatgcatacg gacattatca
     3001 gtctgtggga tcagtctctg aaaccatgtg ttaaactcac cgatctctgc gtaaccttgg
     3061 actgtaataa cgtgacgaat aatggaacta gtgacatgag ggaagagata aagaattgct
     3121 ccttcaatat gacaacagaa ttgcgagaca agcgacaaaa agtatattct ctgttctaca
     3181 aattggacat agtgcagata aatgaggatc aaggtaacag ctccaataac aagtatcgcc
     3241 tgataacttg caacacgtct gcgattaccc aagcatgtcc taaggttaca tttgaaccca
     3301 ttccgattca ttattgcgca cctgcaggct atgctatcct gaagtgcaaa gatgaagagt
     3361 ttaacgggat cggcccgtgc aaaaacgtat ccactgtcca atgcacccac ggtattaagc
     3421 ctgttgtgtc cacacagctc cttctcaatg ggtctctggc agagaaggag gtcaaaataa
     3481 gatgtgaaaa tataacgaac aacgcaaaaa ctatcattgt ccaactggtg aacccagtta
     3541 aaatcaattg cacccgacct aataataata cgagaaagag tatccacatc ggcccagggc
     3601 aggccttcta cgctacaggc gatataatcg gagacataag acaggcgcac tgcaatgtaa
     3661 atcgtacgga atggaataat actctccacc aggtagttga gcagttgcga aagcatttta
     3721 ataaaacgat taactttgct aactcaaccg gaggggactt ggaaataaca acacactcat
     3781 tcaattgcgg gggcgaattt ttttattgta acacgactaa cctgtttaat agcacttgga
     3841 accatacggc tagtatgaac tcaaccgaat ccaatgacac aatcatcctg ccctgtcgaa
     3901 taaaacaaat catcaatatg tggcaaagag tgggccaagc aatgtatgca cccccaataa
     3961 gaggtgtcat aaggtgtgaa tccaatataa ctgggttgat tcttactagg gatggaggaa
     4021 acacgaacag cacccgagaa actttccgcc caggcggggg tgatatgagg gataattggc
     4081 gatcagaact ttacaagtat aaggtagtta agatagaacc cctcggcgtt gctcccacaa
     4141 aagccaagag aagggttgta gaacgcgaaa agcgggccgt agggattggc gctgtattca
     4201 tcggatttct cggagcagct ggatctacta tgggtgcagc ttcaataaca ttgacagttc
     4261 aagcccggca actcctttca ggtattgtac agcagcaaag taacctcctg caagcgattg
     4321 aggctcagca gcatctcctg aagcttacgg tgtggggtat caaacagctt caagcgcggg
     4381 tactcgcggt tgagcgatac cttaaggacc aacaattgct tggaatgtgg ggctgcagcg
     4441 gtaaacttat atgtacaaca tctgtgccgt ggaacagttc ctggagtaac aaatcacaaa
     4501 atgagatttg ggacaatatg acctggttgc agtgggataa agaaatatca aactataccc
     4561 agatcattta cgatctcttg gaggagtctc aaaaccagca ggagaaaaac gaacaggacc
     4621 tcctcgcatt ggataaatgg gccaatttgt ggaactggtt tgacatatct aactggcttt
     4681 ggtatataaa gatattcatc atgattgtag ggggtcttat tggtctgagg atagttttcg
     4741 cagtcttgtc agtcattaac cgagtgcggc agggttattc tcccttgtca tttcaaactc
     4801 acactcctaa tccgagggga ctggataggc ctgagcgaat agaggaagag ggcggtgagc
     4861 aagacaagaa ccggtcaatc aggctcgtgt ctggtttcct ggcactggcg tgggatgatc
     4921 tgaggagtct ttgtcttttc tcataccatc ggttgcgcga cttcatactc atcgtcgcca
     4981 ggaccgtaga acttttggga cattcctcct tgaagggact tcggctcgga tgggagggat
     5041 tgaagtactt ggggaatctg ctgagttatt gggggcggga attgcgaatc tccgccacaa
     5101 atctcttgga tacgattgct atagtaatag ctggatggac ggatagagta atcgaaatag
     5161 ggcagcgcct ctgtcgagca tttctcaata tccccagaag aataaggcaa ggatttgagc
     5221 gcgctctgct ctagtaactc gaggccggca aggccggatc cagacatgat aagatacatt
     5281 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     5341 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     5401 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     5461 taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg
     5521 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5581 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5641 gcgcagccat gaggtcgact ctagacgggg cagtgcatgt aatcccttca gttggttggt
     5701 acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg
     5761 gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg
     5821 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     5881 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     5941 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     6001 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg
     6061 ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc
     6121 atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg
     6181 aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc
     6241 cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca
     6301 gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa
     6361 ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac
     6421 gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata
     6481 taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat
     6541 ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac
     6601 acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa
     6661 gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca
     6721 aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg
     6781 aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt
     6841 tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg
     6901 actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa
     6961 accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc
     7021 gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct
     7081 gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg
     7141 ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     7201 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     7261 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     7321 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     7381 tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa
     7441 tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     7501 tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     7561 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     7621 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     7681 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca
     7741 tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg
     7801 ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat
     7861 tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt
     7921 aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac
     7981 aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg
     8041 ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg
     8101 aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct
     8161 tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg
     8221 gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg
     8281 cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc
     8341 gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca
     8401 atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga
     8461 cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg
     8521 taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg
     8581 gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca
     8641 aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc
     8701 ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg
     8761 gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg
     8821 tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg
     8881 ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt
     8941 tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca
     9001 cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga
     9061 ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct
     9121 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     9181 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tcctgctcct
     9241 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     9301 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     9361 cctgctcctg cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     9421 gcccctcctc ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc ccctcctcct
     9481 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     9541 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     9601 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     9661 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     9721 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     9781 gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa
     9841 cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg
     9901 cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg
     9961 ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct
    10021 cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac
    10081 actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag
    10141 actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag
    10201 atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg
    10261 cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc
    10321 ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc
    10381 ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg
    10441 tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga
    10501 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
    10561 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    10621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    10681 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    10741 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    10801 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    10861 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10921 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
    10981 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    11041 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    11101 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    11161 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    11221 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
    11281 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    11341 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    11401 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    11461 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    11521 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    11581 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    11641 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    11701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    11761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    11821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
    11881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    11941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    12001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    12061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    12121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    12181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    12241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    12301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    12361 tg
//
