LOCUS       sequence-197659-       12920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160
FEATURES             Location/Qualifiers
     source          1..12920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        543..922
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        923..1126
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1077..1097
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1123..1147
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3122..3166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    3756..3890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3780..3799)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3834..3853
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4033..4055)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4615..6404
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6706..8631)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(9066..9084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9152..9256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9257..9325,9326..10117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9475..9494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10288..10876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10777..10796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11494..11639
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11682..12719
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(12717..12736)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12761..12809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
       61 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
      121 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
      181 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
      241 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
      301 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
      361 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
      421 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
      481 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
      541 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      601 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      661 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      721 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      781 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
      841 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
      901 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
      961 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1021 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
     1081 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     1141 tcagatctct agaagctggg taccagctgc tagcgccacc atgcccatgg ggagcttgca
     1201 accgctcgcc actctctatc tcctcggcat gttggtagca tctgtgctgg ctgaggaaaa
     1261 gctctgggtg actgtgtatt acggggtccc agtttggaag gaggctacta ccacgctctt
     1321 ctgcgcctcc gaccggaagg catacgacac cgaggtgcat aatgtgtggg ccacacacgc
     1381 ctgtgtgcct accgacccca acccacagga agtggaactg aagaacgtga ctgagaattt
     1441 caatatgtgg aaaaataata tggtggagca gatgcacgag gacattatta gcctctggga
     1501 tcagtccctt aaaccctgcg tgaaactgac accgctgtgc gtaacgctga attgtactga
     1561 cctccggaat gccacgaacg gaaacgacac aaataccacg tcaagctccc gagggatggt
     1621 gggaggggga gaaatgaaga actgctcatt caatataacg acgaatatcc gagggaaggt
     1681 tcagaaagag tatgccctgt tttacaaact ggacatcgct cctatcgaca acaacagcaa
     1741 taacaggtac cgcctgatta gctgcaacac ttctgtgatt acccaggcct gccccaaggt
     1801 gagcttcgag cctatcccaa tccactactg cgccccagca ggctttgcca tcctgaaatg
     1861 caaggacaag aagttcaacg gcaaaggccc atgtacaaat gtcagtacag tacagtgcac
     1921 acacggtatc cgcccagttg tctctacaca actgctgctg aatggatcct tggcagagga
     1981 agaggtggtg atcagatctg ctaacttcgc cgataatgct aaagtcatca ttgttcagct
     2041 gaacgagtct gtcgaaatca attgtactcg cccaaacaac aacacgcgca agagcattca
     2101 tatcggaccg ggcagggcct tttacacaac aggggagatt attggtgata ttagacaagc
     2161 ccactgcaac ctcagtcgag caaagtggaa tgatactctc aacaaaattg ttatcaaatt
     2221 gcgggaacag tttgggaaca agacaatcgt atttaagcac tcatccggag gcgatccaga
     2281 aatcgtgaca cactctttta actgcggtgg agaatttttc tactgcaact ccacccagct
     2341 gttcaacagt acgtggaacg tgacagagga gtctaacaac accgtggaga ataatacgat
     2401 cacccttcca tgtcggatta agcagatcat caatatgtgg caggaggtgg gaagagcaat
     2461 gtacgcgccg cccattaggg gccagatcag atgcagttca aatatcacag gcctgctcct
     2521 gacacgcgac ggaggcccag aggacaacaa gacagaggtg tttaggcccg gcggcggcga
     2581 catgcgagat aattggaggt ctgaattgta caagtacaag gtggtgaaaa tcgaacccct
     2641 gggagtggcg cctactaagg caaagcgccg cgttgtgcag agagagaaga gggcagtggg
     2701 aattggggcg gtttttcttg ggttcctggg ggctgcaggt agcaccatgg gagcggccgc
     2761 tatgacactg acagtgcaag ccagactcct gctgtctggt attgtccaac agcaaaacaa
     2821 tttgctccgc gcaatagaag cccagcagca cctgctccaa ctgactgtgt ggggcattaa
     2881 acaattgcag gctcgcgtgc tggcagtcga acggtacctg agggatcaac agctcctggg
     2941 catctggggc tgctccggga aactcatctg tacgacagcg gtcccttgga atgccagctg
     3001 gtccaacaaa agcctcaaca agatttggga caatatgacg tggattgagt gggacaggga
     3061 aattaacaat tatacctcaa tcatatattc tctgattgaa gagtcccaga atcagcaaga
     3121 gaagaatgag caggaattgt tggaacttga taaatgggca agcctgtgga actggtttga
     3181 cataaccaaa tggttgtggt atatcaaaat cttcataatg atcgtcggcg gactcatcgg
     3241 gctccgaatt gtgttctcag tcctcagcat tgtgaatagg gtgcgccagg gctattctcc
     3301 cctgagcttc cagacccatc tgccctctag ccgaggacca gataggcctg gtgggatcga
     3361 ggaagaaggc ggggaacggg acagggatag gagtggtcct cttgttaacg ggttcctggc
     3421 acttatatgg gtggacttga gaagcctgtt cctgttctcc tatcataggc tccgggatct
     3481 tctcctgata gtcatgcgga tcgtcgagct gttggggctt gctggcggct gggaagtact
     3541 gaaatattgg tggaatctgt tgcagtattg gagccaggaa cttaagaact ctgctgtgag
     3601 tctccttaat gccacagctg ttgctgtggc cgaaggaacc gatcgagtta ttgaagtact
     3661 gcaacgagca gttcgcgcta ttctgcatat cccacgacgc attcggcagg ggctggagag
     3721 agctctcctg tagtaactcg aggccggcaa ggccggatcc agacatgata agatacattg
     3781 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3841 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     3901 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     3961 aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc gtttcggtga
     4021 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4081 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     4141 cgcagccatg aggtcgactc tagaggatcg atccccgccc cggacgaact aaacctgact
     4201 acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac
     4261 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt
     4321 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc
     4381 tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt
     4441 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg
     4501 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg
     4561 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg
     4621 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat
     4681 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa
     4741 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca
     4801 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt
     4861 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact
     4921 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc
     4981 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata
     5041 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct
     5101 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac
     5161 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga
     5221 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag
     5281 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa
     5341 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt
     5401 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac
     5461 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac
     5521 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg
     5581 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga
     5641 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct
     5701 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5761 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5821 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     5881 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     5941 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt
     6001 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     6061 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     6121 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     6181 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     6241 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata
     6301 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc
     6361 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt gctcccattc
     6421 ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct caccaggtaa
     6481 atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa
     6541 catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag acagatggcc
     6601 agaaatacac caacagcacg catgatgtct actggggatt tattctttag tgcgggggaa
     6661 tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact cctgcccttc
     6721 ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac cctccgcggc
     6781 agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt caaagctgca
     6841 cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt ccttaatcgc
     6901 atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat aacagacaat
     6961 ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa aggggagacg
     7021 actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc ttaggttgta
     7081 aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc cttcgtcggt
     7141 agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa tgttctcaaa
     7201 tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt ttttgcgcct
     7261 gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt catctgcggg
     7321 gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac cttcttggtg
     7381 gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac ctggaggggg
     7441 cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg gcctcttttc
     7501 tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg ctctttcacg
     7561 tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac ctcctcgacc
     7621 ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc ctcctgctcc
     7681 tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     7741 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     7801 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     7861 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
     7921 tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     7981 ccctcctgct cctgcccctc ctgcccctcc tgcccctcct gctcctgccc ctcctcctgc
     8041 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc
     8101 tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
     8161 tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
     8221 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     8281 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc
     8341 ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact
     8401 tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc
     8461 cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt
     8521 atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc
     8581 cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac
     8641 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
     8701 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
     8761 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
     8821 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
     8881 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
     8941 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
     9001 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
     9061 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     9121 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9481 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9781 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     9841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10921 gccttgaagc tgtccctgat ggtcgycgtc kmcctggmta tgttctgcca agggttggtt
    10981 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
    11041 ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
    11101 ccccggaaga aatatatttg catgtcttta gttctatgat gcctctgact tgagcgtcga
    11161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11221 ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc atctacctgc
    11281 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
    11341 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc ctccgttagc
    11401 gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
    11461 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
    11521 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg
    11581 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
    11641 agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
    11701 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    11761 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    11821 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    11881 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
    11941 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    12001 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    12061 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    12121 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    12181 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
    12241 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
    12301 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
    12361 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
    12421 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
    12481 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    12541 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    12601 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    12661 atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa
    12721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    12781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    12841 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    12901 cccaccccac cccccaagtt
//
