LOCUS       sequence-19766-e         426 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Saitoh H, Sparrow DB, Shiomi T, Pu RT, Nishimoto T, Mohun TJ, Dasso 
            M
  TITLE     Ubc9p and the conjugation of SUMO-1 to RanGAP1 and RanBP2.
  JOURNAL   Curr Biol. 1998 Jan 15. 8(2):121-4.
  PUBMED    9427648
REFERENCE   2  (bases 1 to 426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1998 Jan 15. 8(2):121-4."
COMMENT     Sequence Label: pET30 SUMO-1 G97
FEATURES             Location/Qualifiers
     source          1..426
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..108
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             124..138
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gatatacata tgcaccatca tcatcatcat tcttctggtc tggtgccacg cggttctggt
       61 atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt
      121 accgacgacg acgacaaggc catggctgat atcggatccg accaggaggc aaaaccttca
      181 actgaggact tgggggataa gaaggaaggt gaatatatta aactcaaagt cattggacag
      241 gatagcagtg agattcactt caaagtgaaa atgacaacac atctcaagaa actcaaagaa
      301 tcatactgtc aaagacaggg tgtcccaatg aattcactca ggtttctctt tgagggtcag
      361 agaattgctg ataatcatac tccaaaagaa ctgggaatgg aggaagaaga tgtgattgaa
      421 gtttat
//
