LOCUS       sequence-19769-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Wu EW, Clemens KE, Heck DV, Munger K
  TITLE     The human papillomavirus E7 oncoprotein and the cellular 
            transcription factor E2F bind to separate sites on the 
            retinoblastoma tumor suppressor protein.
  JOURNAL   J Virol. 1993 Apr . 67(4):2402-7.
  PUBMED    8445736
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1993 Apr . 67(4):2402-7."
COMMENT     Sequence Label: pGEX2T E7 del DLYC
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(377..399)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(544..562)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctcnatcgg atctggttcc gcgtggatcc atgcatggag atacacctac attgcatgaa
       61 tatatgttag atttgcaacc agagacaaca tatgagcaat taaatgacag ctcagaggag
      121 gaggatgaaa tagatggtcc ggccggacaa gcagaaccgg acagagccca ttacaatatt
      181 gtaacctttt gttgcaagtg tgactctacg cttcggttgt gcgtacaaag cacacacgta
      241 gacattcgta ctttggaaga cctgttaatg ggcacactag gaattgtgtg ccccatctgt
      301 tctcagaaac cataagatga attcatcgtg actgactgac gatctgcctc gcgcgtttcg
      361 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
      421 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
      481 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa
      541 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      601 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      661 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt cantnnttgg
      721 gggggggggg gggnnnnnna tntatncaac anttnnncgg tgnccgccnn tnattcccct
      781 ttttttgccg gcatttttgc ccttccctgg
//
