LOCUS       sequence-197704-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding Venus SX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-Venus SX
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(171..173,174..686)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(213..234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(474..493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     821..842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      925..1011
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1549..2499)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2547..2769
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3261..3355)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3386..4180)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELRDEAARLSWLATTGVPCASVLDVVTETGRDWLLLGEVPGHDLL*
                     FHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3497..3516)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4107..4126
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4359..4377
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atgagcaagg
      181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      301 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      361 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      601 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      661 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
      721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
      781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      841 tgaccgccgc cgggatcgac ctgcagttcg aagttcctat tctctaaatg tccagacctg
      901 caggcatgca agctctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      961 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
     1021 tagacctagg gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt
     1081 tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag
     1141 aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt
     1201 gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca
     1261 agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt
     1321 ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct
     1381 ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca
     1441 tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg
     1501 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc
     1561 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga
     1621 accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag
     1681 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga
     1741 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag
     1801 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg
     1861 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat
     1921 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa
     1981 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt
     2041 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac
     2101 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc
     2161 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat
     2221 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt
     2281 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg
     2341 gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag
     2401 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat
     2461 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg
     2521 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct
     2581 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc
     2641 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt
     2701 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt
     2761 gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt
     2821 tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc
     2881 gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
     2941 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
     3001 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
     3061 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     3121 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     3181 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
     3241 ttactgtccc tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3301 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3361 gagctctcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     3421 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     3481 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     3541 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     3601 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     3661 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     3721 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3781 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3841 atactttctc ggcaggagca aggtgaaatc acaggagatc atgccccggc acttcgccca
     3901 atagcagcca gtcccttccc gtttcagtga caacgtcgag cacagatgcg caaggaacgc
     3961 ccgtcgtggc cagccacgat agccgcgctg cctcgtcccg cagttcattc agggcaccgg
     4021 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     4081 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     4141 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     4201 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     4261 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg
     4321 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4381 ttcgtcttca c
//
