LOCUS       sequence-197760-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of IKZF1 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..1217
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1054..1073
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1265..1289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2721..2745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2792..2881
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        2923..3422
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2958..2981)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3355..3374
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3443..4042
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3443..3462)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3939..3959
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4073..4106)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4162..4750
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4215..4235)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4633..4644)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4753..4769)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4754..4770)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4959..5192
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5264..5385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5301..5320)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5355..5374
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5425..5560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5580..5599)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5581..5599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5622..5980)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5662..5683)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5874..5893
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6007..6111
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6113..6470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6113..6304
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6136..6156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6321..6456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6505..7299
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6505..7299
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6559..6578)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7169..7188
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7487..7506)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7531..7578
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7907..8495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8396..8415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8670..8973
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8975..9173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9128..9148
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9191..9371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9418..9543
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10040..10273
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10458..10502
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10800..10917
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
       61 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
      121 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
      181 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
      241 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
      301 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
      361 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
      421 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
      481 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
      541 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
      601 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
      661 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
      721 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
      781 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
      841 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
      901 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
      961 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     1021 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     1081 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     1141 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     1201 tggctttttt gttagacgct agactgccgg atcgaattgt cgacgaattc ggatctggcc
     1261 aatcacaagt ttgtacaaaa aagttggcat ggatgctgat gagggtcaag acatgtccca
     1321 agtttcaggg aaggaaagcc cccctgtaag cgatactcca gatgagggcg atgagcccat
     1381 gccgatcccc gaggacctct ccaccacctc gggaggacag caaagctcca agagtgacag
     1441 agtcgtggcc agtaatgtta aagtagagac tcagagtgat gaagagaatg ggcgtgcctg
     1501 tgaaatgaat ggggaagaat gtgcggagga tttacgaatg cttgatgcct cgggagagaa
     1561 aatgaatggc tcccacaggg accaaggcag ctcggctttg tcgggagttg gaggcattcg
     1621 acttcctaac ggaaaactaa agtgtgatat ctgtgggatc atttgcatcg ggcccaatgt
     1681 gctcatggtt cacaaaagaa gccacactgg agaacggccc ttccagtgca atcagtgcgg
     1741 ggcctcattc acccagaagg gcaacctgct ccggcacatc aagctgcatt ccggggagaa
     1801 gcccttcaaa tgccacctct gcaactacgc ctgccgccgg agggacgccc tcactggcca
     1861 cctgaggacg cactccgtca ttaaagaaga aactaatcac agtgaaatgg cagaagacct
     1921 gtgcaagata ggatcagaga gatctctcgt gctggacaga ctagcaagta acgtcgccaa
     1981 acgtaagagc tctatgcctc agaaatttct tggggacaag ggcctgtccg acacgcccta
     2041 cgacagcagc gccagctacg agaaggagaa cgaaatgatg aagtcccacg tgatggacca
     2101 agccatcaac aacgccatca actacctggg ggccgagtcc ctgcgcccgc tggtgcagac
     2161 gcccccgggc ggttccgagg tggtcccggt catcagcccg atgtaccagc tgcacaagcc
     2221 gctcgcggag ggcaccccgc gctccaacca ctcggcccag gacagcgccg tggagaacct
     2281 gctgctgctc tccaaggcca agttggtgcc ctcggagcgc gaggcgtccc cgagcaacag
     2341 ctgccaagac tccacggaca ccgagagcaa caacgaggag cagcgcagcg gtctcatcta
     2401 cctgaccaac cacatcgccc cgcacgcgcg caacgggctg tcgctcaagg aggagcaccg
     2461 cgcctacgac ctgctgcgcg ccgcctccga gaactcgcag gacgcgctcc gcgtggtcag
     2521 caccagcggg gagcagatga aggtgtacaa gtgcgaacac tgccgggtgc tcttcctgga
     2581 tcacgtcatg tacaccatcc acatgggctg ccacggcttc cgtgatcctt ttgagtgcaa
     2641 catgtgcggc taccacagcc aggaccggta cgagttctcg tcgcacataa cgcgagggga
     2701 gcaccgcttc cacatgagct acccaacttt cttgtacaaa gtggtgggtg gaggcggttc
     2761 aggtggcgga ggaagcggag gcggtggaag ttacccctac gacgtgcccg actacgccgg
     2821 ctatccgtat gatgtcccgg actatgcagg atcctatcca tacgacgttc cagattacgc
     2881 ttagttaatt aaggccggcc ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     2941 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3001 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3061 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3121 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3181 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     3241 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     3301 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     3361 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     3421 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3481 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3541 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3601 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3661 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3721 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3781 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3841 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3901 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3961 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4021 gacccgcaag cccggtgcct gatgtacaag taggaattcg tcgagggacc taataacttc
     4081 gtatagcata cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa
     4141 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     4201 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4261 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4321 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4381 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4441 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4501 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     4561 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4621 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4681 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4741 gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac
     4801 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     4861 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     4921 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     4981 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5041 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5101 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5161 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5221 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5401 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5461 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5521 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5581 cctatagtga gtcgtattag catgtgagca aaaggccagc ataaatcaaa agaatagacc
     5641 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5701 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5761 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5821 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5881 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5941 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     6001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     6121 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     6481 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6541 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6601 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6661 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     6721 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6781 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6841 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6901 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6961 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     7021 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     7081 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7141 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7201 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7261 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     7321 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7381 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7441 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     7501 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     7561 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     7621 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     7681 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     7741 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8041 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8581 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
     8641 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8701 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8761 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8821 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     8881 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     8941 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     9001 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     9061 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     9121 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
     9181 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9241 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9301 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9361 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     9421 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9481 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9541 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9601 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9661 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9721 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9781 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9841 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
     9901 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9961 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    10021 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    10081 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    10141 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    10201 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    10261 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    10321 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    10381 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    10441 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    10501 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    10561 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    10621 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    10681 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    10741 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
    10801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    10921 gtttattaca gggacagcag agatccagtt tggttagtac cgggcccgct ctagccctag
    10981 atttaaccca acagaaggct cga
//
